Validation of new real-time polymerase chain reaction assays for detection of hepatitis A virus RNA and parvovirus B19 DNA

Transfusion ◽  
2015 ◽  
Vol 56 (2) ◽  
pp. 440-448 ◽  
Author(s):  
Marijke W.A. Molenaar-de Backer ◽  
Mirjam de Waal ◽  
Margret C. Sjerps ◽  
Marco H.G.M. Koppelman
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Parvovirus B19 ◽  
Chain Reaction ◽  
Virus Rna ◽  
Polymerase Chain

Failure to Detect Hepatitis A Virus RNA in Factor VIII Concentrates Using the Polymerase Chain Reaction

Vox Sanguinis ◽  
1994 ◽  
Vol 67 (1) ◽  
pp. 47-50
Author(s):  
F. McOmish ◽  
P.L. Yap ◽  
H. Hart ◽  
K. Peerlinck ◽  
S.M. Burns ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Factor Viii ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Chain Reaction ◽  
Virus Rna ◽  
Factor Viii Concentrates ◽  
Polymerase Chain

scholarly journals Prolonged fecal excretion of hepatitis A virus in adult patients with hepatitis A as determined by polymerase chain reaction

Hepatology ◽  
1996 ◽  
Vol 24 (1) ◽  
pp. 10-13 ◽  
Author(s):  
H Yotsuyanagi ◽  
K Koike ◽  
K Yasuda ◽  
K Moriya ◽  
Y Shintani ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Adult Patients ◽  
Fecal Excretion ◽  
Chain Reaction ◽  
Polymerase Chain

Persistence of Hepatitis A Virus in Oysters†

2003 ◽  
Vol 66 (2) ◽  
pp. 331-334 ◽  
Author(s):  
DAVID H. KINGSLEY ◽  
GARY P. RICHARDS
Keyword(s):  
Polymerase Chain Reaction ◽  
Crassostrea Virginica ◽  
Reverse Transcription ◽  
Hepatitis A ◽  
Infectious Virus ◽  
Hepatitis A Virus ◽  
Complete Removal ◽  
Chain Reaction ◽  
Daily Feeding ◽  
Polymerase Chain

We investigated the ability of hepatitis A virus (HAV) to persist for up to 6 weeks in Eastern oysters (Crassostrea virginica). Viral RNA was detected by reverse transcription–polymerase chain reaction 6 weeks after 16 h of exposure to 90,000 PFU (180 PFU/ml of seawater) of HAV. Assaying for infectious virus in oysters that received a daily feeding of phytoplankton recovered 3,800, 650, and 500 PFU of HAV 1, 2, and 3 weeks after contamination with 90,000 PFU of HAV, respectively. However, no infectious HAV was isolated from oysters 4, 5, or 6 weeks after contamination. These results support the position that shellfish depuration is insufficient for the complete removal of infectious viruses. Extended relay times (in excess of 4 weeks) may be required to produce virologically safe shellfish.

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