Red blood cell depletion from bone marrow and peripheral blood buffy coat: a comparison of two new and three established technologies

Transfusion ◽  
2015 ◽  
Vol 55 (6) ◽  
pp. 1275-1282 ◽  
Author(s):  
Nadine Sorg ◽  
Carolin Poppe ◽  
Milica Bunos ◽  
Eva Wingenfeld ◽  
Christiane Hümmer ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Peripheral Blood ◽  
Buffy Coat ◽  
Cell Depletion

Chromosome Chimerism and Skin Grafts in Bovine Twins and a Shift in the Sex Ratio of Their Progeny

1970 ◽  
Vol 19 (1-2) ◽  
pp. 231-231
Author(s):  
L. Lojda ◽  
L. Černý
Keyword(s):  
Bone Marrow ◽  
Sex Ratio ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Peripheral Blood ◽  
Bone Marrow Cells ◽  
Chromosome Analysis ◽  
Peripheral Blood Lymphocytes ◽  
Skin Grafts ◽  
Cytotoxic Antibodies

Chromosome analysis was made on peripheral blood and bone marrow cells of bovine twins, triplets and quadruplets of different sex. Both sexes showed 60,XY/60,XX chimerism. The percentage of cells with XX and XY chromosomes varied from animal to animal, ranging from 10% to 76%. The differences between the peripheral blood and bone marrow were relatively small. Some males had a majority of cells with female heterochromosomes.This chimerism presumably also affects other tissues, because the progeny of a bull with a 60,XX/60,XY pattern and 68% of cells with XX heterochromosomes showed a marked shift in the sex ratio, namely, 68 ♂: 100 ♀. This evidence suggests that the chimerism affects spermatogonia, which, nevertheless, are able to produce fertile spermatozoa.Immunological studies of the quadruplets showed identity of red blood cell antigens in the A, B, C, FV, L, SU, Z and Ŕ/Ś systems. Cross transplantation of skin grafts among the quadruplets produced not even traces of antibodies to these red blood cell antigens, and no cytotoxic antibodies to peripheral blood lymphocytes in these animals.

scholarly journals Infusion hemolysis after pediatric major ABO-mismatched bone marrow transplant: Comparison of two red blood cell depletion techniques

2017 ◽  
Vol 65 (3) ◽  
pp. e26883
Author(s):  
Robert Sheppard Nickel ◽  
Muna Qayed ◽  
Diana Worthington-White ◽  
Sean R. Stowell ◽  
Kuang-Yueh Chiang
Keyword(s):  
Bone Marrow ◽  
Blood Cell ◽  
Bone Marrow Transplant ◽  
Red Blood Cell ◽  
Marrow Transplant ◽  
Cell Depletion

Equine bone marrow volume reduction, red blood cell depletion, and mononuclear cell recovery using the PrepaCyte-CB processing system

2015 ◽  
Vol 44 (2) ◽  
pp. 188-193 ◽  
Author(s):  
Andrew G. Burton ◽  
Kaitlin C. Clark ◽  
Dori L. Borjesson ◽  
Danielle D. Carrade ◽  
Julie Burges ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Blood Cell ◽  
Mononuclear Cell ◽  
Red Blood Cell ◽  
Processing System ◽  
Volume Reduction ◽  
Cell Depletion ◽  
Cell Recovery ◽  
Equine Bone

scholarly journals Evaluation of qPCR on blood and skin microbiopsies, peripheral blood buffy coat smear, and urine antigen ELISA for diagnosis and test of cure for visceral leishmaniasis in HIV-coinfected patients in India: a prospective cohort study

BMJ Open ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. e042519
Author(s):  
Sophie I Owen ◽  
Sakib Burza ◽  
Shiril Kumar ◽  
Neena Verma ◽  
Raman Mahajan ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Visceral Leishmaniasis ◽  
Hiv Infection ◽  
Peripheral Blood ◽  
Medical Science ◽  
Bone Marrow Aspiration ◽  
Buffy Coat ◽  
Tropical Medicine ◽  
Urine Antigen ◽  
Antigen Elisa

IntroductionHIV coinfection presents a challenge for diagnosis of visceral leishmaniasis (VL). Invasive splenic or bone marrow aspiration with microscopic visualisation of Leishmania parasites remains the gold standard for diagnosis of VL in HIV-coinfected patients. Furthermore, a test of cure by splenic or bone marrow aspiration is required as patients with VL-HIV infection are at a high risk of treatment failure. However, there remain financial, implementation and safety costs to these invasive techniques which severely limit their use under field conditions.Methods and analysisWe aim to evaluate blood and skin qPCR, peripheral blood buffy coat smear microscopy and urine antigen ELISA as non-invasive or minimally invasive alternatives for diagnosis and post-treatment test of cure for VL in HIV-coinfected patients in India, using a sample of 91 patients with parasitologically confirmed symptomatic VL-HIV infection.Ethics and disseminationEthical approval for this study has been granted by The Liverpool School of Tropical Medicine, The Institute of Tropical Medicine in Antwerp, the University of Antwerp and the Rajendra Memorial Research Institute of Medical Science in Patna. Any future publications will be published in open access journals.Trial registration numberCTRI/2019/03/017908.

scholarly journals Optimization of the Aldehyde Dehydrogenase-Based Cord Blood Potency Assay Using Red Blood Cell Depletion

2016 ◽  
Vol 22 (3) ◽  
pp. S424-S425
Author(s):  
Kevin Shoulars ◽  
Tracy Gentry ◽  
Ben Lam ◽  
Bert Wognum ◽  
Steve Szilvassy ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cord Blood ◽  
Aldehyde Dehydrogenase ◽  
Red Blood Cell ◽  
Cell Depletion ◽  
Potency Assay
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