Monoclonal anti-A activity against the FORS1 (Forssman) antigen

Transfusion ◽  
2014 ◽  
Vol 55 (1) ◽  
pp. 129-136 ◽  
Author(s):  
Katie Barr ◽  
Elena Korchagina ◽  
Inna Popova ◽  
Nicolai Bovin ◽  
Stephen Henry
Keyword(s):  
Forssman Antigen

Crystal structure of galabiose: an internal part of the Forssman antigen

1984 ◽  
Vol 40 (a1) ◽  
pp. C65-C65
Author(s):  
G. Svensson ◽  
J. Albertsson ◽  
C. Svensson ◽  
G. Magnusson
Keyword(s):  
Crystal Structure ◽  
Internal Part ◽  
Forssman Antigen

scholarly journals Subcellular localization of Forssman glycolipid in epithelial MDCK cells by immuno-electronmicroscopy after freeze-substitution.

1991 ◽  
Vol 115 (4) ◽  
pp. 1009-1019 ◽  
Author(s):  
I L van Genderen ◽  
G van Meer ◽  
J W Slot ◽  
H J Geuze ◽  
W F Voorhout
Keyword(s):  
Plasma Membrane ◽  
Mdck Cells ◽  
Protein A ◽  
Freeze Substitution ◽  
Apical Plasma Membrane ◽  
Double Labeling ◽  
Transport Vesicles ◽  
Forssman Antigen ◽  
I Cells ◽  
Mdck I

Forssman antigen, a neutral glycosphingolipid carrying five monosaccharides, was localized in epithelial MDCK cells by the immunogold technique. Labeling with a well defined mAb and protein A-gold after freeze-substitution and low temperature embedding in Lowicryl HM20 of aldehyde-fixed and cryoprotected cells, resulted in high levels of specific labeling and excellent retention of cellular ultrastructure compared to ultra-thin cryosections. No Forssman glycolipid was lost from the cells during freeze-substitution as measured by radio-immunostaining of lipid extracts. Redistribution of the glycolipid between membranes did not occur. Forssman glycolipid, abundantly expressed on the surface of MDCK II cells, did not move to neighboring cell surfaces in cocultures with Forssman negative MDCK I cells, even though they were connected by tight junctions. The labeling density on the apical plasma membrane was 1.4-1.6 times higher than basolateral. Roughly two-thirds of the gold particles were found intracellularly. The Golgi complex was labeled for Forssman as were endosomes, identified by endocytosed albumin-gold, and lysosomes, defined by double labeling for cathepsin D. In most cases, the nuclear envelope was Forssman positive, but the labeling density was 10-fold less than on the plasma membrane. Mitochondria and peroxisomes, the latter identified by catalase, remained free of label, consistent with the notion that they do not receive transport vesicles carrying glycosphingolipids. The present method of lipid immunolabeling holds great potential for the localization of other antigenic lipids.

Forssman expression on human erythrocytes: biochemical and genetic evidence of a new histo-blood group system

Blood ◽  
2013 ◽  
Vol 121 (8) ◽  
pp. 1459-1468 ◽  
Author(s):  
Lola Svensson ◽  
Annika K. Hult ◽  
Robert Stamps ◽  
Jonas Ångström ◽  
Susann Teneberg ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Group ◽  
Blood Cells ◽  
Human Erythrocytes ◽  
Blood Group System ◽  
Group System ◽  
Glycolipid Antigen ◽  
Human Red Blood Cells ◽  
Key Points ◽  
Forssman Antigen

Key Points A new histo-blood group system was discovered, based on the identification of Forssman glycolipid antigen on human red blood cells. A newly described polymorphism in the GBGT1 gene activates the encoded enzyme to synthesize Forssman antigen.

scholarly journals PRIMARY SERUM TOXICITY AS DEMONSTRATED BY THE CHICKEN EMBRYO

1935 ◽  
Vol 61 (4) ◽  
pp. 489-499 ◽  
Author(s):  
Ernst Witebsky ◽  
Erwin Neter
Keyword(s):  
Guinea Pig ◽  
Human Serum ◽  
Test Object ◽  
Chicken Embryo ◽  
Normal Serum ◽  
Vascular Network ◽  
Inhibitory Influence ◽  
Suitable Test ◽  
Forssman Antigen ◽  
Normal Human

1. The 3 day old chicken embryo removed from its shell is a suitable test object for the demonstration of primary serum toxicity. Addition of normal rabbit type sera as well as Forssman antiserum causes the vascular network to contract and the embryo sinks in the yolk and dies. 2. Only sera of animals of the so called rabbit type produce this phenomenon. Sera of the guinea pig type are ineffective. 3. Heating to 51°C. destroys the complement content of normal human serum as also its effectiveness to produce the vascular phenomenon. 4. Up to the present it has not been possible to reactivate heat-inactivated normal serum by the addition of complement, while inactivated Forssman antiserum can be easily reactivated. 5. The vascular phenomenon of the chicken embryo is produced not only by the addition of a mixture of Forssman antiserum and complement but also by separate addition of both components. 6. Guinea pig type sera, containing dissolved Forssman antigen, are not only ineffective but actually exert an inhibitory influence on effective rabbit type sera as well as on Forssman antiserum.

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