Riboflavin and ultraviolet light treatment of platelets triggers p38MAPK signaling: inhibition significantly improves in vitro platelet quality after pathogen reduction treatment

Transfusion ◽  
2013 ◽  
Vol 53 (12) ◽  
pp. 3164-3173 ◽  
Author(s):  
Peter Schubert ◽  
Danielle Coupland ◽  
Brankica Culibrk ◽  
Raymond P. Goodrich ◽  
Dana V. Devine
Keyword(s):  
Ultraviolet Light ◽  
Light Treatment ◽  
Reduction Treatment ◽  
P38mapk Signaling ◽  
Pathogen Reduction

Pathogen reduction treatment using riboflavin and ultraviolet light impairs platelet reactivity toward specific agonists in vitro

Transfusion ◽  
2014 ◽  
Vol 54 (9) ◽  
pp. 2292-2300 ◽  
Author(s):  
Sabrina Zeddies ◽  
Iris M. De Cuyper ◽  
Pieter F. van der Meer ◽  
Brunette B. Daal ◽  
Dirk de Korte ◽  
...  
Keyword(s):  
Ultraviolet Light ◽  
Platelet Reactivity ◽  
Reduction Treatment ◽  
Pathogen Reduction

scholarly journals Complete Inactivation of Blood Borne Pathogen Trypanosoma cruzi in Stored Human Platelet Concentrates and Plasma Treated With 405 nm Violet-Blue Light

2020 ◽  
Vol 7 ◽  
Author(s):  
Katarzyna I. Jankowska ◽  
Rana Nagarkatti ◽  
Nirmallya Acharyya ◽  
Neetu Dahiya ◽  
Caitlin F. Stewart ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Human Plasma ◽  
Blue Light ◽  
Chagas Disease ◽  
Light Treatment ◽  
Platelet Concentrates ◽  
Complete Inactivation ◽  
Pathogen Reduction ◽  
Biochemical Indices

The introduction of pathogen reduction technologies (PRTs) to inactivate bacteria, viruses and parasites in donated blood components stored for transfusion adds to the existing arsenal toward reducing the risk of transfusion-transmitted infectious diseases (TTIDs). We have previously demonstrated that 405 nm violet-blue light effectively reduces blood-borne bacteria in stored human plasma and platelet concentrates. In this report, we investigated the microbicidal effect of 405 nm light on one important bloodborne parasite Trypanosoma cruzi that causes Chagas disease in humans. Our results demonstrated that a light irradiance at 15 mWcm−2 for 5 h, equivalent to 270 Jcm−2, effectively inactivated T. cruzi by over 9.0 Log10, in plasma and platelets that were evaluated by a MK2 cell infectivity assay. Giemsa stained T. cruzi infected MK2 cells showed that the light-treated parasites in plasma and platelets were deficient in infecting MK2 cells and did not differentiate further into intracellular amastigotes unlike the untreated parasites. The light-treated and untreated parasite samples were then evaluated for any residual infectivity by injecting the treated parasites into Swiss Webster mice, which did not develop infection even after the animals were immunosuppressed, further demonstrating that the light treatment was completely effective for inactivation of the parasite; the light-treated platelets had similar in vitro metabolic and biochemical indices to that of untreated platelets. Overall, these results provide a proof of concept toward developing 405 nm light treatment as a pathogen reduction technology (PRT) to enhance the safety of stored human plasma and platelet concentrates from bloodborne T. cruzi, which causes Chagas disease.

Effects of riboflavin and ultraviolet light treatment on pathogen reduction and platelets

Transfusion ◽  
2020 ◽  
Vol 60 (11) ◽  
pp. 2647-2654
Author(s):  
Yundi Yin ◽  
Ling Li ◽  
Li Gong ◽  
Haixia Xu ◽  
Zhong Liu
Keyword(s):  
Ultraviolet Light ◽  
Light Treatment ◽  
Pathogen Reduction

Riboflavin-ultraviolet light pathogen reduction treatment does not impact the immunogenicity of murine red blood cells

Transfusion ◽  
2015 ◽  
Vol 56 (4) ◽  
pp. 863-872 ◽  
Author(s):  
Christopher A. Tormey ◽  
Manjula Santhanakrishnan ◽  
Nicole H. Smith ◽  
Jingchun Liu ◽  
Susanne Marschner ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Ultraviolet Light ◽  
Blood Cells ◽  
Reduction Treatment ◽  
Pathogen Reduction

scholarly journals Treatment of blood with a pathogen reduction technology using ultraviolet light and riboflavin inactivates Ebola virus in vitro

Transfusion ◽  
2016 ◽  
Vol 56 ◽  
pp. S6-S15 ◽  
Author(s):  
Andrew P. Cap ◽  
Heather F. Pidcoke ◽  
Shawn D. Keil ◽  
Hilary M. Staples ◽  
Manu Anantpadma ◽  
...  
Keyword(s):  
Ultraviolet Light ◽  
Ebola Virus ◽  
Pathogen Reduction

Ultrastructural Changes in Three Different Mammalian Cells Following Ultraviolet Laser Irradiation

1972 ◽  
Vol 30 ◽  
pp. 350-351
Author(s):  
K. Shankar Narayan ◽  
Kailash C. Gupta ◽  
Tohru Okigaki
Keyword(s):  
Ultraviolet Light ◽  
Mammalian Cells ◽  
Biological Effects ◽  
Survival Rates ◽  
Chinese Hamster ◽  
Cell Types ◽  
Differential Sensitivity ◽  
Ultrastructural Changes ◽  
Ultraviolet Laser

The biological effects of short-wave ultraviolet light has generally been described in terms of changes in cell growth or survival rates and production of chromosomal aberrations. Ultrastructural changes following exposure of cells to ultraviolet light, particularly at 265 nm, have not been reported.We have developed a means of irradiating populations of cells grown in vitro to a monochromatic ultraviolet laser beam at a wavelength of 265 nm based on the method of Johnson. The cell types studies were: i) WI-38, a human diploid fibroblast; ii) CMP, a human adenocarcinoma cell line; and iii) Don C-II, a Chinese hamster fibroblast cell strain. The cells were exposed either in situ or in suspension to the ultraviolet laser (UVL) beam. Irradiated cell populations were studied either "immediately" or following growth for 1-8 days after irradiation.Differential sensitivity, as measured by survival rates were observed in the three cell types studied. Pattern of ultrastructural changes were also different in the three cell types.

Corneal endothelial safety after ultraviolet light treatment of the light-adjustable intraocular lens

2011 ◽  
Vol 37 (2) ◽  
pp. 324-327 ◽  
Author(s):  
Alejandro Lichtinger ◽  
Christopher A. Sandstedt ◽  
Karina Padilla ◽  
Daniel M. Schwartz ◽  
Arturo S. Chayet
Keyword(s):  
Ultraviolet Light ◽  
Intraocular Lens ◽  
Light Treatment

Inhibition of X-Ray- and Ultraviolet Light-Induced Transformation in Vitro by Modifiers of Poly(ADP-Ribose) Synthesis

1984 ◽  
Vol 99 (2) ◽  
pp. 219 ◽  
Author(s):  
Carmia Borek ◽  
Augustinus Ong ◽  
William F. Morgan ◽  
James E. Cleaver
Keyword(s):  
Ultraviolet Light ◽  
X Ray
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