scholarly journals Retrograde trafficking and plasma membrane recycling pathways of the budding yeast Saccharomyces cerevisiae

Traffic ◽  
2019 ◽  
Vol 21 (1) ◽  
pp. 45-59 ◽  
Author(s):  
Mengxiao Ma ◽  
Christopher G. Burd
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Plasma Membrane ◽  
Budding Yeast ◽  
Membrane Recycling ◽  
Yeast Saccharomyces Cerevisiae ◽  
Retrograde Trafficking

Localization of a 210-kDa microtubule-interacting protein in the yeast Saccharomyces cerevisiae

1992 ◽  
Vol 38 (2) ◽  
pp. 149-152 ◽  
Author(s):  
J. Hašek ◽  
J. Jochová ◽  
P. Dráber ◽  
V. Viklický ◽  
E. Streiblová
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Cycle ◽  
Monoclonal Antibody ◽  
Plasma Membrane ◽  
Key Words ◽  
Budding Yeast ◽  
Yeast Saccharomyces Cerevisiae ◽  
Double Labeling ◽  
Interacting Protein ◽  
Cytoplasmic Microtubules

Using the monoclonal antibody MA-01, which recognizes a 210-kDa protein in cell-free extracts, spindle and cytoplasmic microtubules were visualized in budding yeast, Saccharomyces cerevisiae. In additional, a spot-like staining was found beneath the plasma membrane, revealing in part correlation with F-actin distribution. This pattern was common for cells of all cell-cycle stages. The interaction of the protein recognized by MA-01 with microtubules was confirmed in the double labeling with a polyclonal antitubulin antibody and by the sensitivity of intranuclear structures stained by MA-01 to the microtubule disrupting drug nocodazole. Key words: immunoblotting, immunofluorescence, microtubule-interacting protein, Saccharomyces cerevisiae.

scholarly journals Seg1 controls eisosome assembly and shape

2012 ◽  
Vol 198 (3) ◽  
pp. 405-420 ◽  
Author(s):  
Karen E. Moreira ◽  
Sebastian Schuck ◽  
Bianca Schrul ◽  
Florian Fröhlich ◽  
James B. Moseley ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Plasma Membrane ◽  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Budding Yeast ◽  
Yeast Saccharomyces Cerevisiae ◽  
Normal Plasma ◽  
Cytoplasmic Proteins ◽  
Stable Domains

Eisosomes are stable domains at the plasma membrane of the budding yeast Saccharomyces cerevisiae and have been proposed to function in endocytosis. Eisosomes are composed of two main cytoplasmic proteins, Pil1 and Lsp1, that form a scaffold around furrow-like plasma membrane invaginations. We show here that the poorly characterized eisosome protein Seg1/Ymr086w is important for eisosome biogenesis and architecture. Seg1 was required for efficient incorporation of Pil1 into eisosomes and the generation of normal plasma membrane furrows. Seg1 preceded Pil1 during eisosome formation and established a platform for the assembly of other eisosome components. This platform was further shaped and stabilized upon the arrival of Pil1 and Lsp1. Moreover, Seg1 abundance controlled the shape of eisosomes by determining their length. Similarly, the Schizosaccharomyces pombe Seg1-like protein Sle1 was necessary to generate the filamentous eisosomes present in fission yeast. The function of Seg1 in the stepwise biogenesis of eisosomes reveals striking architectural similarities between eisosomes in yeast and caveolae in mammals.

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