scholarly journals Pex20p functions as the receptor for non‐PTS1/non‐PTS2 acyl‐CoA oxidase import into peroxisomes of the yeast Yarrowia lipolytica

Traffic ◽  
2019 ◽  
Vol 20 (7) ◽  
pp. 504-515 ◽  
Author(s):  
Jinlan Chang ◽  
Richard A. Rachubinski
Keyword(s):  
Yarrowia Lipolytica ◽  
Acyl Coa Oxidase ◽  
Yeast Yarrowia Lipolytica

scholarly journals Peroxisome division in the yeast Yarrowia lipolytica is regulated by a signal from inside the peroxisome

2003 ◽  
Vol 162 (7) ◽  
pp. 1255-1266 ◽  
Author(s):  
Tong Guo ◽  
Yuriy Y. Kit ◽  
Jean-Marc Nicaud ◽  
Marie-Therese Le Dall ◽  
S. Kelly Sears ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Matrix Proteins ◽  
Peroxisomal Protein ◽  
Organelle Division ◽  
The Matrix ◽  
Membrane Bound ◽  
Complete Set ◽  
Acyl Coa Oxidase ◽  
Peroxisome Division ◽  
Yeast Yarrowia Lipolytica

We describe an unusual mechanism for organelle division. In the yeast Yarrowia lipolytica, only mature peroxisomes contain the complete set of matrix proteins. These mature peroxisomes assemble from several immature peroxisomal vesicles in a multistep pathway. The stepwise import of distinct subsets of matrix proteins into different immature intermediates along the pathway causes the redistribution of a peroxisomal protein, acyl-CoA oxidase (Aox), from the matrix to the membrane. A significant redistribution of Aox occurs only in mature peroxisomes. Inside mature peroxisomes, the membrane-bound pool of Aox interacts with Pex16p, a membrane-associated protein that negatively regulates the division of early intermediates in the pathway. This interaction inhibits the negative action of Pex16p, thereby allowing mature peroxisomes to divide.

Purification and Characterization of the Recombinant Form of Acyl CoA Oxidase 3 from the Yeast Yarrowia lipolytica

2000 ◽  
Vol 384 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Yi-Shan Luo ◽  
Hui-Jie Wang ◽  
K.V Gopalan ◽  
D.K Srivastava ◽  
Jean-Marc Nicaud ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Recombinant Form ◽  
Purification And Characterization ◽  
Acyl Coa Oxidase ◽  
Yeast Yarrowia Lipolytica

scholarly journals Acyl-CoA oxidase is imported as a heteropentameric, cofactor-containing complex into peroxisomes of Yarrowia lipolytica

2002 ◽  
Vol 156 (3) ◽  
pp. 481-494 ◽  
Author(s):  
Vladimir I. Titorenko ◽  
Jean-Marc Nicaud ◽  
Huijie Wang ◽  
Honey Chan ◽  
Richard A. Rachubinski
Keyword(s):  
Yarrowia Lipolytica ◽  
Matrix Protein ◽  
Protein Targeting ◽  
Polypeptide Chain ◽  
Pivotal Role ◽  
The Matrix ◽  
Peroxisomal Targeting ◽  
Acyl Coa Oxidase ◽  
Yeast Yarrowia Lipolytica

Five isoforms of acyl-CoA oxidase (Aox), designated Aox1p to Aox5p, constitute a 443-kD heteropentameric complex containing one polypeptide chain of each isoform within the peroxisomal matrix of the yeast Yarrowia lipolytica. Assembly of the Aox complex occurs in the cytosol and precedes its import into peroxisomes. Peroxisomal targeting of the Aox complex is abolished in a mutant lacking the peroxin Pex5p, a component of the matrix protein targeting machinery. Import of the Aox complex into peroxisomes does not involve the cytosolic chaperone Pex20p, which mediates the oligomerization and import of peroxisomal thiolase. Aox2p and Aox3p play a pivotal role in the formation of the Aox complex in the cytosol and can substitute for one another in promoting assembly of the complex. In vitro, these subunits retard disassembly of the Aox complex and increase the efficiency of its reassembly. Neither Aox2p nor Aox3p is required for acquisition of the cofactor FAD by other components of the complex. We provide evidence that the Aox2p- and Aox3p-assisted assembly of the Aox complex in the cytosol is mandatory for its import into peroxisomes and that no component of the complex can penetrate the peroxisomal matrix as a monomer.

scholarly journals Psychrotrophic yeast Yarrowia lipolytica NCYC 789 mediates the synthesis of antimicrobial silver nanoparticles via cell-associated melanin

AMB Express ◽  
2013 ◽  
Vol 3 (1) ◽  
pp. 32 ◽  
Author(s):  
Mugdha Apte ◽  
Devashree Sambre ◽  
Shital Gaikawad ◽  
Swanand Joshi ◽  
Ashok Bankar ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Yarrowia Lipolytica ◽  
Yeast Yarrowia Lipolytica

scholarly journals Stable isotope and chemical inhibition analyses suggested the existence of a non-mevalonate-like pathway in the yeast Yarrowia lipolytica

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sivamoke Dissook ◽  
Tomohisa Kuzuyama ◽  
Yuri Nishimoto ◽  
Shigeru Kitani ◽  
Sastia Putri ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Oleaginous Yeast ◽  
Isoprenoid Biosynthesis ◽  
Mep Pathway ◽  
Authentic Standard ◽  
Chemical Inhibition ◽  
Chromatographic Peaks ◽  
Methyl Erythritol Phosphate ◽  
Limiting Condition ◽  
Yeast Yarrowia Lipolytica

AbstractMethyl erythritol phosphate (MEP) is the metabolite found in the MEP pathway for isoprenoid biosynthesis, which is known to be utilized by plants, algae, and bacteria. In this study, an unprecedented observation was found in the oleaginous yeast Yarrowia lipolytica, in which one of the chromatographic peaks was annotated as MEP when cultivated in the nitrogen limiting condition. This finding raised an interesting hypothesis of whether Y. lipolytica utilizes the MEP pathway for isoprenoid biosynthesis or not, because there is no report of yeast harboring the MEP pathway. Three independent approaches were used to investigate the existence of the MEP pathway in Y. lipolytica; the spiking of the authentic standard, the MEP pathway inhibitor, and the 13C labeling incorporation analysis. The study suggested that the mevalonate and MEP pathways co-exist in Y. lipolytica and the nitrogen limiting condition triggers the utilization of the MEP pathway in Y. lipolytica.

scholarly journals Droplet-based microfluidic high-throughput screening of heterologous enzymes secreted by the yeast Yarrowia lipolytica

2017 ◽  
Vol 16 (1) ◽  
Author(s):  
Thomas Beneyton ◽  
Stéphane Thomas ◽  
Andrew D. Griffiths ◽  
Jean-Marc Nicaud ◽  
Antoine Drevelle ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
High Throughput ◽  
High Throughput Screening ◽  
Yeast Yarrowia Lipolytica

scholarly journals pH level has a strong impact on population dynamics of the yeast Yarrowia lipolytica and oil micro-droplets in multiphasic bioreactor

2018 ◽  
Vol 365 (16) ◽  
Author(s):  
Doria Naila Bouchedja ◽  
Sabine Danthine ◽  
Tambi Kar ◽  
Patrick Fickers ◽  
Hosni Sassi ◽  
...  
Keyword(s):  
Population Dynamics ◽  
Yarrowia Lipolytica ◽  
Strong Impact ◽  
Ph Level ◽  
Yeast Yarrowia Lipolytica
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