Precise estimation of chlorophyll a , b and carotenoid content by deconvolution of the absorption spectrum and new simultaneous equations for Chl determination

2021 ◽  
Author(s):  
Marie Chazaux ◽  
Christo Schiphorst ◽  
Gioele Lazzari ◽  
Stefano Caffarri
Keyword(s):  
Absorption Spectrum ◽  
Chlorophyll A ◽  
Simultaneous Equations ◽  
Carotenoid Content ◽  
Precise Estimation

scholarly journals Impact of Moroccan Crocus sativus L. tepals, corms, and stigmas extract on growth and photosynthetic pigments in tomato seedling

2021 ◽  
Vol 35 (2) ◽  
Author(s):  
Amine Khoulati ◽  
E. Saalaoui
Keyword(s):  
Chlorophyll A ◽  
Foliar Application ◽  
Positive Impact ◽  
Crocus Sativus ◽  
Carotenoid Content ◽  
Chlorophyll B ◽  
Tomato Seedling ◽  
Tomato Plants ◽  
By Products ◽  
Crocus Sativus L

An experiment was carried out in a greenhouse to study the effect of aqueous extracts of Crocus sativus L. by-products on tomato plants. Three concentrations of tepals and corms were used by fertigation: 1 g/L, 2 g/L, and 3 g/L. The aqueous extract of the stigmas was used as a foliar application at 0.6 g/L. The experiment was carried out in a completely randomized block with three repetitions for each concentration. The concentration of tepal extract at 3 g/L significantly (p≤0.05) increased the plants' height, the chlorophyll a, b content. The same results were observed for the foliar treatment with stigmas; however, there was no effect of tepal extract on the carotenoid content. On the other hand, the concentration 2 g/L of the corms extract had a positive impact (p≤0.05) in the chlorophyll b content while the concentration of 3 g/L increased the plant's height, the chlorophyll a (p≤0.05). Current results indicate that Crocus sativus by-products could improve certain physiological aspects of the recipient plants and new and natural biostimulants.

The Absorption Spectrum of Chlorophyll a Crystals

1954 ◽  
Vol 22 (1) ◽  
pp. 142-142
Author(s):  
Earl E. Jacobs ◽  
A. Stanley Holt
Keyword(s):  
Absorption Spectrum ◽  
Chlorophyll A

scholarly journals ВМІСТ ФОТОСИНТЕТИЧНИХ ПІГМЕНТІВ У ЛИСТКАХ ГРЕЧКИ ЗА ДІЇ БІОЛОГІЧНИХ ПРЕПАРАТІВ

2021 ◽  
Vol 81 (1-2) ◽  
pp. 83-89
Author(s):  
V. P. Karpenko ◽  
I. I. Mostoviak ◽  
A. A. Datsenko ◽  
R. M. Prytuliak ◽  
O. I. Zabolotnyi
Keyword(s):  
Plant Growth ◽  
Chlorophyll A ◽  
Plant Development ◽  
Seed Treatment ◽  
Carotenoid Content ◽  
Chlorophyll B ◽  
Biochemical Processes ◽  
Chlorophyll A And B ◽  
Microbial Preparation ◽  
Physiological And Biochemical

The article presents the results of a field experiment to study the effects of different rates of the microbial preparation Diazobacterin under different methods of using the plant growth regulator Radostim (seed treatment before sowing and spraying crops) on the content of chlorophyll a and b in buckwheat leaves of Yelena variety, their sum and carotenoids. Determination of chlorophyll a and b, their sum and carotenoids was performed in selected samples of buckwheat leaves in the field conditions, in the phases of stem branching and the beginning of flowering according to the method described by V.F. Gavrylenko and T.V. Zhyhalova using a spectrophotometer. The pigment concentration was calculated from Holm-Wettstein's equations for 100 % acetone. It was found that the content of photosynthetic pigments in buckwheat leaves is conditioned by the weather, rates and methods of application of the studied microbial preparations and phases of plant development. In particular, the analysis of chlorophyll and carotenoid content in the early flowering phase of buckwheat plants showed a significant increase compared to the phase of stem branching, which may be due to increased physiological and biochemical processes in plants, against the background of improving mineral nutrition, growth and plant development. Thus, with the multiple use of the microbial preparation Diazobacterin in the rates of 150, 175 and 200 ml and the stimulator of plant growth Radostim in the rate of 250 ml / t for seed treatment before sowing, the content of chlorophylls a and b, their sum and carotenoids with increasing application of Diazobacterin increased and the excess relative to control ranged within: 29–31 % for chlorophyll a; 10–11 % for chlorophyll b; 24–26 % for their sum and 23–24 % for carotenoids. At the same time, with a complex application for pre-sowing seed treatment of a mixture of the microbial preparation Diazobacterin in the rate of 200 ml per hectare of seeds with the stimulator of plant growth Radostym in the rate of 250 ml / t followed by spraying on this background crops the stimulator of plant growth Radostim in the rate of 50 ml / ha buckwheat leaves form the highest content of chlorophyll a in comparison with the control (40 %); chlorophyll b (15 %); the sum of chlorophyll a + b (33 %) and carotenoids (29 %). These data indicate the creation of more favorable conditions for physiological and biochemical processes in plants, including photosynthesis, with the direct positive effect of which, functionally active pigment complex of the leaf apparatus of buckwheat is formed.

scholarly journals AKTIVITAS TERMOPROTEKSI DAN FOTOPROTEKSI EKSTRAK KASAR KAROTENOID MESOKARP KELAPA SAWIT (Elaeis guineensis Jacq.) TERHADAP KESTABILAN KLOROFIL-A)

2019 ◽  
Vol 12 (1) ◽  
pp. 112-123
Author(s):  
Dece Elisabeth Sahertian ◽  
Indriatmoko Indriatmoko ◽  
Leenawaty Limantara ◽  
Tatas Hardo Panintingjati Brotosudarmo
Keyword(s):  
Absorption Spectrum ◽  
Light Intensity ◽  
Chlorophyll A ◽  
Elaeis Guineensis ◽  
Heating Time ◽  
Protective Function ◽  
Stability And Instability ◽  
In Series ◽  
Elaeis Guineensis Jacq ◽  
The Stability

AbstrakKelapa sawit (Elaeis guineensis Jacq.) merupakan tanaman yang mengandung karotenoid tinggi pada mesokarp yang merupakan bagian dari buah. Cahaya dan suhu dalam proses fotosintesis memberi pengaruh bagi kestabilan dan ketidakstabilan karotenoid dan klorofil. Penelitian ini bertujuan untuk mengetahui aktivitas fotostabilitas dan termostabilitas ekstrak karotenoid mesokarp terhadap klorofil-a yang diiradiasi dengan lampu volpi (intralux 4100) daylight pada intensitas cahaya 31960 lux, 47040 lux dan 76640 lux dalam seri waktu penyinaran 0, 5, 10, 15, 20, 25, dan 30 menit serta dipanaskan pada suhu 25, 50, 65, dan 90 °C dengan seri waktu pemanasan 0, 1, 2, 3, 6, 9, dan 24 jam. Pengukuran spektrum serapan tiap perlakuan diukur menggunakan Spektrofotometer UV-Tampak pada panjang gelombang 300–800 nm. Hasil penelitian menunjukkan bahwa karotenoid tidak melakukan fungsi proteksi dengan baik pada perlakuan pemanasan 65 °C dan 90 °C selama 24 jam, serta perlakuan iradiasi hingga 30 menit pada ketiga intensitas cahaya. Kesimpulan dari penelitian ini adalah klorofil-a murni lebih stabil pada perlakuan pemanasan dan iradiasi. Kemampuan proteksi karotenoid terhadap kestabilan klorofil-a yaitu pada suhu di bawah 50 °C dan pada intensitas cahaya di bawah 31960 lux.Abstract Oil palm (Elaeis guineensis Jacq.) is a plant that contains high carotenoids in its mesocarp which is part of the fruit. Light and temperature in photosynthesis may affect the stability and instability of carotenoid and chlorophyll. The aim of this research was to examine the thermostability and photostability activities of the carotenoids in mesocarp extract on chlorophyll which were irradiated by Volpi lamp (4100 intralux) daylight at light intensity of 31960 lux, 47040 lux and 76640 lux in series of time radiation 0, 5, 10, 15, 20, 25, and 30 minutes and heated at 25, 50, 65, and 90°C with series of heating time 0, 1, 2, 3, 6, 9, and 24 hours. Measurement of the absorption spectrum of each treatment was measured using a UV-Vis Spectrophotometer at a wavelength range of 300–800 nm. The results showed the protective function of carotenoids did not perform well at the heating treatments of 65°C and 90°C in 24 hours and at the irradiation for 30 minutes in all the light intensities. In conclusion, pure chlorophyll-a is more stable in heating and irradiation treatments. Carotenoid protection ability against chlorophyll-a is at temperatures below 50 °C and at a light intensity below 31960 lux.

scholarly journals CONTENT OF PHOTOSYNTHETIC PIGMENTS IN ONTOGENESIS OF WILD AND CULTURAL PEA

2021 ◽  
Vol 16 (4) ◽  
pp. 10-14
Author(s):  
Sergey Bobkov ◽  
Ivan Bychkov
Keyword(s):  
Chlorophyll A ◽  
Carotenoid Content ◽  
Wild Accession ◽  
Scientific Center ◽  
Bud Formation ◽  
Seed Filling ◽  
Chlorophylls A And B ◽  
New Varieties ◽  
Wild Accessions ◽  
High Level

The objective consisted in study of wild pea representatives on chlorophyll and carotenoid content for use in producing new varieties with high photosynthetic efficiency and optimal assimilate distribution. The research was conducted in 2016‑2017 in Federal Scientific Center of Legumes and Groat Crops (Orel). Plants were grown on experimental field with density 1.2 million plants/ha on triplicated plots 1 m2. Wild pea accessions k-5322 (asiaticum), k-3370 (elatius), k-4014 (elatius) from VIR collection and varieties Temp and Stabil were used. Wild accessions k-3370 and k-5322 had more chlorophyll a, chlorophyll b and carotenoids then leafy Temp variety on 24.5 %, 28.2 %, 41.5 % and 13.3 %, 2.7 %, 18.7 % respectively. Unlike the cultivated pea, accessions k-3370 and k-5322 retain high level of chlorophylls a and b in ontogenesis longer time. After transition from bud formation to the beginning of seed filling content of chlorophylls a and b in accession k-3370 was at the same level (11.71 mg/g и 11.1 mg/g respectively), in accession k-5322 it decreased insignificantly from 11.31 mg/g to 9.72 mg/g. Nevertheless, in variety Temp content of chlorophylls a and b significantly decreased on 34.1 % after transition from bud formation to the seed filling. Wild accession k-3370 like pea varieties had low chlorophyll a/b ratio (2.92), but accession k-5322 had the highest value (3.26) of the ratio. In wild accession k-3370 and k-5322 the chlorophyll a/b ratio was changed in ontogenesis that is no-typical for Temp variety. Pea wild accessions can be considered as sources of valuable alleles determining the high level of chlorophylls and carotenoids

scholarly journals The Effects of Serendipita indica and Guanidine-Modified Nanomaterial on Growth and Development of Cabbage Seedlings and Black Spot Infestation

Agriculture ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1295
Author(s):  
Sana Saleem ◽  
Zuzana Bytešníková ◽  
Lukáš Richtera ◽  
Robert Pokluda
Keyword(s):  
Plant Growth ◽  
Chlorophyll A ◽  
Seed Treatment ◽  
Vegetation Index ◽  
Normalized Difference Vegetation Index ◽  
Positive Impact ◽  
Disease Incidence ◽  
Black Spot ◽  
Carotenoid Content ◽  
Combined Application

To ensure sustainable agricultural production and protection of crops from various biotic and abiotic stresses, while keeping in view environmental protection, by minimal usage of chemicals, the exploitation of beneficial microorganisms and modern nanotechnologies in the field of agriculture is of paramount importance. This study aimed to investigate the effects of Serendipita indica and guanidine-modified nanomaterial on the growth, and other selected parameters, of cabbage, as well as incidence of black spot disease. S. indica was applied in substrate and by seed inoculation. S. indica had a positive impact on the development of plants, and resulted in reduced black spot severity. The maximum plant height (119 mm) and number of leaves (8.3) were observed in S. indica-treated plants. Pigments were enhanced, i.e., chlorophyll a (0.79 mg/g), chlorophyll b (0.22 mg/g), and carotenoid content (0.79 mg/g), by substrate treatment. The highest antioxidant capacity (9.5 mM/L), chlorophyll a and b (1.8 and 0.6 mg/g), and carotenoid content (1.8 mg/L) were reported in S. indica seed treatment. S. indica treatment resulted in 59% and 41% disease incidence decrease in substrate and seed treatment, respectively. Guanidine-modified nanomaterial was seen to be effective in improving plant growth and reducing disease incidence; however, it did not perform better than S. indica. Application of nanoparticles resulted in enhanced normalized difference vegetation index and fluorescence by increasing chlorophyll a, b, and carotenoid content. Nitrogen content was the highest in plants treated with nanoparticles. However, the effect of the combined application of fungus and nanoparticles was similar to that of S. indica alone in substrate treatment, although negative impacts were reported in the biochemical parameters of cabbage. S. indica has great potential to enhance plant growth and manage Alternaria incidence in cabbage crops.

scholarly journals Genetic Association between Foliage Yield and Contributing Traits in Vegetable Chenopods: Implications for Genetic Improvement

2018 ◽  
Vol 47 (1) ◽  
pp. 24-33
Author(s):  
Atul BHARGAVA ◽  
Francisco FUENTES ◽  
Sudhir SHUKLA ◽  
Shilpi SRIVASTAVA ◽  
Deepak OHRI
Keyword(s):  
Ascorbic Acid ◽  
Protein Content ◽  
Chlorophyll A ◽  
Positive Association ◽  
Leaf Size ◽  
Negative Association ◽  
Stem Diameter ◽  
Carotenoid Content ◽  
Chlorophyll B ◽  
Positive Direct

A two-year study was conducted to evaluate the foliage yield potential in 13 germplasm lines of Chenopodium album for 3 successive cuttings. Correlations among foliage yield and its contributing traits, along with path analysis was also worked out. Foliage yield was maximum for C. album IC 107297, followed by C. album H.P. and C. album amaranticolor. The genotype × year interaction was non-significant for all the traits except stem diameter and moisture content. Leaf size, plant height and stem diameter showed significant positive correlation with foliage yield both at phenotypic and genotypic levels in all the cuttings. Chlorophyll a and chlorophyll b showed positive association with carotenoid content and negative association with ascorbic acid in all the cuttings as well as on pooled basis. Significant negative association was observed between leaves/plant and foliage yield at genotypic level in all the cuttings (Ist cutting: -0.472*; IInd cutting: -0.414*; IIIrd cutting: -0.480*) as well as on pooled basis (-0.591**). Protein content negatively affected foliage yield in all the cuttings. Fibre content had high negative value of direct path for pooled data but positively influenced foliage yield indirectly via leaves/plant, stem diameter, chlorophyll a, chlorophyll b and protein content. Ascorbic acid positively affected yield in Ist cutting as well as on pooled basis. Leaf size had high positive direct effect and significant positive association with foliage yield that indicates a true relationship between these traits. Leaf size also indirectly affected foliage yield in a positive direction through majority of other traits. Thus, direct selection for leaf size should be exercised to bring about improvement in foliage yield in C. album.

Changes in photosynthetic pigments in relation to soils contaminated with industrial activities in Cassia occidentalis L.

2016 ◽  
Vol 39 (3) ◽  
pp. 231-233
Author(s):  
Manpreet Kaur ◽  
Meena Bakshi ◽  
Renu Bhardwaj
Keyword(s):  
Chlorophyll A ◽  
Photosynthetic Pigments ◽  
Contaminated Soils ◽  
Industrial Area ◽  
Cement Industry ◽  
Carotenoid Content ◽  
Small Scale ◽  
Control Soil ◽  
Cassia Occidentalis ◽  
Different Soils

Rapid industrialization in Bhagwanpur industrial area (Uttarakhand) severely affected soil. Various industries such as cement industry, electroplating industries, small scale steel industries are contaminating environment in several ways. Efforts were made to study the biochemical changes in response to three different soils in Cassia occidentalis L. CRD pot culture experiment was laid out via using three different soil types viz. industrial area soil, control soil and mixed soil (50% control soil: 50% industrial area soil). Biochemical constituents like proteins and photosynthetic pigments viz. chlorophyll ‘a’, chlorophyll ‘b’, total chlorophylls and carotenoids were analysed on 30th day of culture. Significant differences (0.05%) were observed among different soils used. Contaminated soils drastically reduced pigments as well as protein content. Maximum protein, chlorophyll content and chlorophyll a/b ratio were observed in plants raised in control soil. In contrast, the carotenoid content was maximum in plants grown in mixed soil.

scholarly journals The Qualitative Differences for Photosynthetic Content of Common Bean (Phaseolus vulgaris L.) Populations  in Kosovo

2014 ◽  
Vol 6 (3) ◽  
pp. 331-334
Author(s):  
Sali Ali ALIU ◽  
Imer RUSINOVCI ◽  
Shukri FETAHU ◽  
Bekim GASHI
Keyword(s):  
Common Bean ◽  
Chlorophyll A ◽  
Room Temperature ◽  
Genetic Material ◽  
Carotenoid Content ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Breeding Programs ◽  
Chl A ◽  
Acetone Water

Genetic diversity analysis of common bean populations is useful for breeding programs, as it helps to select genetic material to be used for further crossings. Twenty (20) common bean populations were analyzed using qualitative traits, chlorophyll “a” (Chl ‘a’), chlorophyll “b” (Chl ‘b’), total chlorophyll “a+b” (Total Chl) and carotenoides. The design of the experiment was conducted with leaves of common bean collected from different regions of Kosovo. The experiment was completely randomly with four repetitions. Pigments were extracted by grinding 80-100 mg freshly sampled leaves in 80% (v/v) acetone/water containing MgCO3, at room temperature, preserved in the dark for 24 hours. Concentration of chlorophyll and carotenoid content was measured by spectrophotometer using absorbance recorded at 663 nm, 644 nm and 452.3 nm for maximum absorption of Chl ‘a’, Chl ‘b’, and carotenoids respectively. According to our data the differences between populations for Chl ‘a’, and Chl ‘b’ was significantly higher at level of probability LSDp=0.01. The average values for Chl ‘a’, was 1.67 mg.g-1, while for Chl‘b’was 0.74 mg.g-1. In addition, the results for carotenoids content between populations were with high differences.

Sign in / Sign up

Export Citation Format

Share Document