Integration of the SMXL/D53 strigolactone signalling repressors in the model of shoot branching regulation in Pisum sativum

2021 ◽  
Author(s):  
Stephanie C. Kerr ◽  
Suyash Patil ◽  
Alexandre de Saint Germain ◽  
Jean‐Paul Pillot ◽  
Julie Saffar ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Shoot Branching ◽  
Strigolactone Signalling

scholarly journals Dynamics of Strigolactone Function and Shoot Branching Responses in Pisum sativum

2013 ◽  
Vol 6 (1) ◽  
pp. 128-140 ◽  
Author(s):  
Elizabeth A. Dun ◽  
Alexandre de Saint Germain ◽  
Catherine Rameau ◽  
Christine A. Beveridge
Keyword(s):  
Pisum Sativum ◽  
Shoot Branching

scholarly journals Sucrose represses the expression of the strigolactone signalling gene D3/RMS4/MAX2 to promote tillering

2020 ◽  
Author(s):  
Suyash B. Patil ◽  
Francois F. Barbier ◽  
Jinfeng Zhao ◽  
Syed Adeel Zafar ◽  
Muhammad Uzair ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Inhibitory Effect ◽  
Shoot Branching ◽  
Over Expression ◽  
Feeding Experiments ◽  
Signalling Network ◽  
Shoot Architecture ◽  
Dicotyledonous Plants ◽  
Strigolactone Signalling ◽  
High Sucrose

AbstractShoot branching, which is regulated by a complex signalling network, is a major component of plant architecture and therefore of crop yield. Sugars, acting in a network with hormones, have recently emerged as key players in the control of shoot branching. Previous studies in dicotyledonous plants have shown that sucrose suppresses the inhibitory effect of the plant hormone strigolactone (SL) during this process. The molecular mechanisms underlying this effect are unknown. Here we show that sucrose could antagonise the suppressive action of SL on tillering in rice. At the mechanistic level, we revealed that sucrose alleviates SL-mediated degradation of D53. Increase in sucrose availability inhibits the expression of D3, which encodes the orthologue of the arabidopsis F-box MAX2 required for SL signalling. Over-expression of D3 prevented sucrose from inhibiting D53 degradation and enabled the SL inhibition of tillering under high sucrose. The enhanced bud elongation of the d3 mutant to sucrose treatment indicates that suppressed SL perception reduces the minimum amount of sucrose required for sustained bud outgrowth. Decapitation and sugar feeding experiments in pea indicate that RMS4, the D3/MAX2 orthologue in pea, is also involved in the interactions between sucrose and SL. This work shows that D3/MAX2/RMS4 is a key component in the integrating both SL and sugar pathways during the regulation of shoot architecture.

scholarly journals HEXOKINASE1 interferes with cytokinin synthesis and strigolactone perception during sugar-induced shoot branching

2020 ◽  
Author(s):  
Francois F. Barbier ◽  
Da Cao ◽  
Franziska Fichtner ◽  
Christoph Weiste ◽  
Maria-Dolores Perez-Garcia ◽  
...  
Keyword(s):  
Plant Architecture ◽  
Negative Impact ◽  
List Type ◽  
Shoot Branching ◽  
Shoot Architecture ◽  
Sugar Signalling ◽  
Cytokinin Treatment ◽  
Cytokinin Synthesis ◽  
Strigolactone Signalling ◽  
Dependent Pathway

ABSTRACT-Plant architecture is controlled by several endogenous signals including hormones and sugars. However, only little is known about the nature and roles of the sugar signalling pathways in this process. Here we test whether the sugar pathway mediated by HEXOKINASE1 (HXK1) is involved in the control of shoot branching.-To test the involvement of HXK1 in the control of shoot architecture we modulated the HXK1 pathway using physiological and genetic approaches in diverse plants, rose, arabidopsis and pea and evaluated impacts of hormonal pathways.-We show that triggering a hexokinase-dependent pathway was able to promote bud outgrowth in pea and rose. In arabidopsis, both HXK1 deficiency and defoliation led to decreased shoot branching and conferred hypersensitivity to auxin. HXK1 expression was positively correlated with sugar availability. HXK1-deficient plants displayed decreased cytokinin levels and increased expression of MAX2 which is required for strigolactone signalling. The branching phenotype of HXK1-deficient plants could be partly restored by cytokinin treatment and strigolactone deficiency could override the negative impact of HXK1 deficiency on shoot branching.-Our observations demonstrate that a HXK1-dependent pathway contributes to the regulation of shoot branching and interact with hormones to modulate plant architecture.

scholarly journals Strigolactone regulates shoot development through a core signalling pathway

2016 ◽  
Author(s):  
Tom Bennett ◽  
Yueyang Liang ◽  
Madeleine Seale ◽  
Sally Ward ◽  
Dörte Müller ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Ubiquitin Ligase ◽  
Dominant Mode ◽  
Shoot Branching ◽  
Genetic Approach ◽  
Downstream Targets ◽  
Developmental Responses ◽  
Related Proteins ◽  
Strigolactone Signalling ◽  
Auxin Efflux Carrier

ABSTRACTStrigolactones are a recently identified class of hormone that regulate multiple aspects of plant development. The DWARF14 (D14) α/β fold protein has been identified as a strigolactone receptor, which can act through the SCFMAX2 ubiquitin ligase, but the universality of this mechanism is not clear. Multiple proteins have been suggested as targets for strigolactone signalling, including both direct proteolytic targets of SCFMAX2, and downstream targets. However, the relevance and importance of these proteins to strigolactone signalling in many cases has not been fully established. Here we assess the contribution of these targets to strigolactone signalling in adult shoot developmental responses. We find that all examined strigolactone responses are regulated by SCFMAX2 and D14, and not by other D14-like proteins. We further show that all examined strigolactone responses likely depend on degradation of SMXL proteins in the SMXL6 clade, and not on other proposed proteolytic targets. Taken together, our results suggest that in the adult shoot, the dominant mode of strigolactone signalling is D14-initiated, MAX2-mediated degradation of SMXL6-related proteins. We confirm that the BRANCHED1 transcription factor and the PIN-FORMED1 auxin efflux carrier are plausible downstream targets of this pathway in the regulation of shoot branching, and show that BRC1 likely acts in parallel to PIN1.AUTHOR SUMMARYStrigolactones are a recently discovered family plant hormones with diverse roles in development, most strikingly in the regulation shoot branching. Our understanding of the mechanism(s) by which plants perceive and respond to strigolactones is growing rapidly. It is likely that the strigolactone signaling pathway has evolved by duplication and diversification of specific components of a pre-existing pathway, involved in perception and response to an as yet unknown hormone. Several of these components have been identified and several new candidate components have been implicated in the pathway. We have adopted a genetic approach to assess systematically the contributions of all these players to strigolactone signaling in the shoot. We exclude some of the candidate proteins from involvement in strigolactone-mediated shoot branching control and define a core pathway for strigolactone action in the shoot. We provide evidence that downstream of this core, the strigolactone signaling pathway branches, with different effectors mediating different shoot responses.

Gene expression during two alternative pathways of ovary development in Pisum sativum: fruit development and ovary senescence

1992 ◽  
Vol 85 (1) ◽  
pp. 69-76 ◽  
Author(s):  
Maria-Jose Sanchez-Beltran ◽  
Juan Carbonell ◽  
Jose L. Garcia-Martinez ◽  
Isabel Lopez-Diaz
Keyword(s):  
Gene Expression ◽  
Pisum Sativum ◽  
Fruit Development ◽  
Ovary Development ◽  
Alternative Pathways ◽  
Ovary Senescence
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