scholarly journals Robotic Assay for Drought (RoAD): An Automated Phenotyping System for Brassinosteroid and Drought Response

2021 ◽  
Author(s):  
Lirong Xiang ◽  
Trevor M. Nolan ◽  
Yin Bao ◽  
Mitch Elmore ◽  
Taylor Tuel ◽  
...  
Keyword(s):  
Drought Response ◽  
Automated Phenotyping

scholarly journals EMS Derived Wheat Mutant BIG8-1 (Triticum aestivum L.)—A New Drought Tolerant Mutant Wheat Line

2021 ◽  
Vol 22 (10) ◽  
pp. 5314
Author(s):  
Marlon-Schylor L. le Roux ◽  
Nicolas Francois V. Burger ◽  
Maré Vlok ◽  
Karl J. Kunert ◽  
Christopher A. Cullis ◽  
...  
Keyword(s):  
Amino Acids ◽  
Water Deficit ◽  
Triticum Aestivum L ◽  
Water Deficit Stress ◽  
Drought Response ◽  
Fibrous Root ◽  
Wheat Varieties ◽  
Breeding Programs ◽  
Response Characteristics ◽  
Drought Tolerant

Drought response in wheat is considered a highly complex process, since it is a multigenic trait; nevertheless, breeding programs are continuously searching for new wheat varieties with characteristics for drought tolerance. In a previous study, we demonstrated the effectiveness of a mutant known as RYNO3936 that could survive 14 days without water. In this study, we reveal another mutant known as BIG8-1 that can endure severe water deficit stress (21 days without water) with superior drought response characteristics. Phenotypically, the mutant plants had broader leaves, including a densely packed fibrous root architecture that was not visible in the WT parent plants. During mild (day 7) drought stress, the mutant could maintain its relative water content, chlorophyll content, maximum quantum yield of PSII (Fv/Fm) and stomatal conductance, with no phenotypic symptoms such as wilting or senescence despite a decrease in soil moisture content. It was only during moderate (day 14) and severe (day 21) water deficit stress that a decline in those variables was evident. Furthermore, the mutant plants also displayed a unique preservation of metabolic activity, which was confirmed by assessing the accumulation of free amino acids and increase of antioxidative enzymes (peroxidases and glutathione S-transferase). Proteome reshuffling was also observed, allowing slow degradation of essential proteins such as RuBisCO during water deficit stress. The LC-MS/MS data revealed a high abundance of proteins involved in energy and photosynthesis under well-watered conditions, particularly Serpin-Z2A and Z2B, SGT1 and Calnexin-like protein. However, after 21 days of water stress, the mutants expressed ABC transporter permeases and xylanase inhibitor protein, which are involved in the transport of amino acids and protecting cells, respectively. This study characterizes a new mutant BIG8-1 with drought-tolerant characteristics suited for breeding programs.

Variability in drought response among the plus tree accessions of Tectona grandis (Linn f.) from the provenances of Kerala, South India

2021 ◽  
Vol 43 (3) ◽  
Author(s):  
Mohammed Ali Eltoum Hassan ◽  
A. V. Santhoshkumar ◽  
T. K. Hrideek ◽  
C. M. Jijeesh ◽  
Jiji Joseph
Keyword(s):  
South India ◽  
Tectona Grandis ◽  
Drought Response ◽  
Plus Tree

Drought response in winter wheat: protection from oxidative stress and mutagenesis effect

2021 ◽  
Vol 43 (1) ◽  
Author(s):  
Elisaveta Kirova ◽  
Dobrina Pecheva ◽  
Lyudmila Simova-Stoilova
Keyword(s):  
Oxidative Stress ◽  
Winter Wheat ◽  
Drought Response

Nitrogen addition amplifies the nonlinear drought response of grassland productivity to extended growing‐season droughts

Ecology ◽  
2021 ◽  
Author(s):  
Bo Meng ◽  
Junqin Li ◽  
Gregory E. Maurer ◽  
Shangzhi Zhong ◽  
Yuan Yao ◽  
...  
Keyword(s):  
Growing Season ◽  
Nitrogen Addition ◽  
Drought Response ◽  
Grassland Productivity

scholarly journals Ectopic Overexpression of Histone H3K4 Methyltransferase CsSDG36 from Tea Plant Decreases Hyperosmotic Stress Tolerance in Arabidopsis thaliana

2021 ◽  
Vol 22 (10) ◽  
pp. 5064
Author(s):  
Qinghua Chen ◽  
Linghui Guo ◽  
Yanwen Yuan ◽  
Shuangling Hu ◽  
Fei Guo ◽  
...  
Keyword(s):  
Drought Stress ◽  
Transmembrane Domain ◽  
Tea Plant ◽  
Microtubule Assembly ◽  
Pcr Analysis ◽  
Drought Response ◽  
Stomatal Development ◽  
Wild Type ◽  
Over Expression ◽  
Histone H3k4

Histone methylation plays an important regulatory role in the drought response of many plants, but its regulatory mechanism in the drought response of the tea plant remains poorly understood. Here, drought stress was shown to induce lower relative water content and significantly downregulate the methylations of histone H3K4 in the tea plant. Based on our previous analysis of the SET Domain Group (SDG) gene family, the full-length coding sequence (CDS) of CsSDG36 was cloned from the tea cultivar ‘Fuding Dabaicha’. Bioinformatics analysis showed that the open reading frame (ORF) of the CsSDG36 gene was 3138 bp, encoding 1045 amino acids and containing the conserved structural domains of PWWP, PHD, SET and PostSET. The CsSDG36 protein showed a close relationship to AtATX4 of the TRX subfamily, with a molecular weight of 118,249.89 Da, and a theoretical isoelectric point of 8.87, belonging to a hydrophilic protein without a transmembrane domain, probably located on the nucleus. The expression of CsSDG36 was not detected in the wild type, while it was clearly detected in the over-expression lines of Arabidopsis. Compared with the wild type, the over-expression lines exhibited lower hyperosmotic resistance by accelerating plant water loss, increasing reactive oxygen species (ROS) pressure, and increasing leaf stomatal density. RNA-seq analysis suggested that the CsSDG36 overexpression caused the differential expression of genes related to chromatin assembly, microtubule assembly, and leaf stomatal development pathways. qRT-PCR analysis revealed the significant down-regulation of stomatal development-related genes (BASL, SBT1.2(SDD1), EPF2, TCX3, CHAL, TMM, SPCH, ERL1, and EPFL9) in the overexpression lines. This study provides a novel sight on the function of histone methyltransferase CsSDG36 under drought stress.

Fungal Symbionts Alter Plant Drought Response

2012 ◽  
Vol 65 (3) ◽  
pp. 671-678 ◽  
Author(s):  
Elise R. Worchel ◽  
Hannah E. Giauque ◽  
Stephanie N. Kivlin
Keyword(s):  
Drought Response

Drought response QTLs in a Super Basmati × Azucena population by high‐density GBS‐based SNP linkage mapping

2021 ◽  
Author(s):  
Raheela Waheed ◽  
John Carlos Ignacio ◽  
Juan David Arbelaez ◽  
Venice Margarette Juanillas ◽  
Muhammad Asif ◽  
...  
Keyword(s):  
Linkage Mapping ◽  
High Density ◽  
Drought Response

scholarly journals Water uptake dynamics under progressive drought stress in diverse accessions of the OryzaSNP panel of rice (Oryza sativa)

2012 ◽  
Vol 39 (5) ◽  
pp. 402 ◽  
Author(s):  
Veeresh R. P. Gowda ◽  
Amelia Henry ◽  
Vincent Vadez ◽  
H. E. Shashidhar ◽  
Rachid Serraj
Keyword(s):  
Oryza Sativa ◽  
Drought Stress ◽  
Root Growth ◽  
Water Uptake ◽  
Root Length Density ◽  
Oryza Sativa L ◽  
Root Water Uptake ◽  
Snp Markers ◽  
Drought Response ◽  
Genotypic Differences

In addition to characterising root architecture, evaluating root water uptake ability is important for understanding drought response. A series of three lysimeter studies were conducted using the OryzaSNP panel, which consists of 20 diverse rice (Oryza sativa L.) genotypes. Large genotypic differences in drought response were observed in this genotype panel in terms of plant growth and water uptake. Total water uptake and daily water uptake rates in the drought-stress treatment were correlated with root length density, especially at depths below 30 cm. Patterns of water uptake among genotypes remained consistent throughout the stress treatments: genotypes that initially extracted more water were the same genotypes that extracted more water at the end of the study. These results suggest that response to drought by deep root growth, rather than a conservative soil water pattern, seems to be important for lowland rice. Genotypes in the O. sativa type aus group showed some of the greatest water uptake and root growth values. Since the OryzaSNP panel has been genotyped in detail with SNP markers, we expect that these results will be useful for understanding the genetics of rice root growth and function for water uptake in response to drought.

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