OsMTD2‐Mediated Reactive Oxygen Species (ROS) Balance is Essential for Intact Pollen Tube Elongation in Rice

2021 ◽  
Author(s):  
Yu‐Jin Kim ◽  
Myung‐Hee Kim ◽  
Woo‐Jong Hong ◽  
Sunok Moon ◽  
Sun Tae Kim ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Pollen Tube ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Pollen Tube Elongation

Reactive oxygen species are involved in pollen tube initiation in kiwifruit

Plant Biology ◽  
2011 ◽  
pp. no-no ◽  
Author(s):  
A. Speranza ◽  
R. Crinelli ◽  
V. Scoccianti ◽  
A. Geitmann
Keyword(s):  
Reactive Oxygen Species ◽  
Pollen Tube ◽  
Reactive Oxygen ◽  
Oxygen Species

scholarly journals Ca2+-Activated Reactive Oxygen Species Production by Arabidopsis RbohH and RbohJ Is Essential for Proper Pollen Tube Tip Growth

2014 ◽  
Vol 26 (3) ◽  
pp. 1069-1080 ◽  
Author(s):  
Hidetaka Kaya ◽  
Ryo Nakajima ◽  
Megumi Iwano ◽  
Masahiro M. Kanaoka ◽  
Sachie Kimura ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Pollen Tube ◽  
Reactive Oxygen Species Production ◽  
Tip Growth ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Species Production

scholarly journals Arabidopsis ABCG28 is required for the apical accumulation of reactive oxygen species in growing pollen tubes

2019 ◽  
Vol 116 (25) ◽  
pp. 12540-12549 ◽  
Author(s):  
Thanh Ha Thi Do ◽  
Hyunju Choi ◽  
Michael Palmgren ◽  
Enrico Martinoia ◽  
Jae-Ung Hwang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Tip Growth ◽  
Ectopic Expression ◽  
Reactive Oxygen ◽  
Pollen Tubes ◽  
Tube Growth ◽  
Oxygen Species ◽  
Ros Accumulation

Tip-focused accumulation of reactive oxygen species (ROS) is tightly associated with pollen tube growth and is thus critical for fertilization. However, it is unclear how tip-growing cells establish such specific ROS localization. Polyamines have been proposed to function in tip growth as precursors of the ROS, hydrogen peroxide. The ABC transporter AtABCG28 may regulate ROS status, as it contains multiple cysteine residues, a characteristic of proteins involved in ROS homeostasis. In this study, we found that AtABCG28 was specifically expressed in the mature pollen grains and pollen tubes. AtABCG28 was localized to secretory vesicles inside the pollen tube that moved toward and fused with the plasma membrane of the pollen tube tip. Knocking out AtABCG28 resulted in defective pollen tube growth, failure to localize polyamine and ROS to the growing pollen tube tip, and complete male sterility, whereas ectopic expression of this gene in root hair could recover ROS accumulation at the tip and improved the growth under high-pH conditions, which normally prevent ROS accumulation and tip growth. Together, these data suggest that AtABCG28 is critical for localizing polyamine and ROS at the growing tip. In addition, this function of AtABCG28 is likely to protect the pollen tube from the cytotoxicity of polyamine and contribute to the delivery of polyamine to the growing tip for incorporation into the expanding cell wall.

scholarly journals Reactive oxygen species mediate pollen tube rupture to release sperm for fertilization in Arabidopsis

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Qiaohong Duan ◽  
Daniel Kita ◽  
Eric A. Johnson ◽  
Mini Aggarwal ◽  
Laura Gates ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Pollen Tube ◽  
Reactive Oxygen ◽  
Oxygen Species

scholarly journals PbrRALF2-elicited reactive oxygen species signaling is mediated by the PbrCrRLK1L13-PbrMPK18 module in pear pollen tubes

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Xiaobing Kou ◽  
Jiangmei Sun ◽  
Peng Wang ◽  
Danqi Wang ◽  
Peng Cao ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Reactive Oxygen ◽  
Pollen Tubes ◽  
Tube Growth ◽  
General Mechanism ◽  
Dependent Manner ◽  
Ros Production ◽  
Oxygen Species

AbstractRapid alkalinization factors (RALFs) are cysteine-rich peptides that play important roles in a variety of biological processes, such as cell elongation and immune signaling. Recent studies in Arabidopsis have shown that RALFs regulate pollen tube growth via plasma membrane receptor-like kinases (RLKs). However, the downstream signal transduction mechanisms of RLKs in pollen tubes are unknown. Here, we identified PbrRALF2, a pear (Pyrus bretschneideri) pollen RALF peptide that inhibits pollen tube growth. We found that PbrRALF2 interacts with a malectin-like domain-containing RLK, PbrCrRLK1L13. The relative affinity between PbrRALF2 and PbrCrRLK1L13 was at the submicromolar level, which is consistent with the values of ligand–receptor kinase pairs and the physiological concentration for PbrRALF2-mediated inhibition of pollen tube growth. After binding to its extracellular domain, PbrRALF2 activated the phosphorylation of PbrCrRLK1L13 in a dose-dependent manner. We further showed that the MAP kinase PbrMPK18 is a downstream target of PbrCrRLK1L13 that mediates PbrRALF2-elicited reactive oxygen species (ROS) production. The excessive accumulation of ROS inhibits pollen tube growth. We show that MPK acts as a mediator for CrRLK1L to stimulate ROS production, which might represent a general mechanism by which RALF and CrRLK1L function in signaling pathways.

scholarly journals The Peptide PbrPSK2 From Phytosulfokine Family Induces Reactive Oxygen Species (ROS) Production to Regulate Pear Pollen Tube Growth

2020 ◽  
Vol 11 ◽  
Author(s):  
Xiaobing Kou ◽  
Qian Liu ◽  
Yangyang Sun ◽  
Peng Wang ◽  
Shaoling Zhang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Pollen Tube ◽  
Gene Family ◽  
Pollen Tube Growth ◽  
Prunus Persica ◽  
Reactive Oxygen ◽  
Tube Growth ◽  
Evolutionary Analysis ◽  
Dependent Manner ◽  
Oxygen Species

Phytosulfokines (PSKs) are plant peptide growth factors that participate in multiple biological processes, including cell elongation and immune signaling. However, little is known about PSKs in Rosaceae species. Here, we identified 10 PSK genes in pear (Pyrus bretschneideri), 11 in apple (Malus × domestica), four in peach (Prunus persica), six in strawberry (Fragaria vesca), and five in Chinese plum (Prunus mume). In addition, we undertook comparative analysis of the PSK gene family in pear and the four other species. Evolutionary analysis indicated that whole genome duplication events (WGD) may have contributed to the expansion of the PSK gene family in Rosaceae. Transcriptomes, reverse transcription-PCR and quantitative real-time-PCR analyses were undertaken to demonstrate that PbrPSK2 is highly expressed in pear pollen. In addition, by adding purified E. coli-expressed PbrPSK2 to pollen and using an antisense oligonucleotide approach, we showed that PbrPSK2 can promote pear pollen tube elongation in a dose-dependent manner. Furthermore, PbrPSK2 was found to mediate the production of reactive oxygen species to regulate pear pollen tube growth.

Reactive oxygen species produced by NADPH oxidase are involved in pollen tube growth

2007 ◽  
Vol 174 (4) ◽  
pp. 742-751 ◽  
Author(s):  
Martin Potocký ◽  
Mark A. Jones ◽  
Radek Bezvoda ◽  
Nicholas Smirnoff ◽  
Viktor Žárský
Keyword(s):  
Reactive Oxygen Species ◽  
Pollen Tube ◽  
Nadph Oxidase ◽  
Pollen Tube Growth ◽  
Reactive Oxygen ◽  
Tube Growth ◽  
Oxygen Species
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