scholarly journals Identification of transcription factors controlling cell wall invertase gene expression for reproductive development via bioinformatic and transgenic analyses

2021 ◽  
Author(s):  
Jun Li ◽  
Ryan Foster ◽  
Si Ma ◽  
Sheng‐Jin Liao ◽  
Samuel Bliss ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Wall ◽  
Transcription Factors ◽  
Reproductive Development ◽  
Cell Wall Invertase ◽  
Invertase Gene

scholarly journals Induction of a Pea Cell-Wall Invertase Gene by Wounding and Its Localized Expression in Phloem

1996 ◽  
Vol 112 (3) ◽  
pp. 1111-1117 ◽  
Author(s):  
L. Zhang ◽  
N. S. Cohn ◽  
J. P. Mitchell
Keyword(s):  
Cell Wall ◽  
Cell Wall Invertase ◽  
Invertase Gene

Roles played by invertase and gene expression in the development of the horn-shaped gall on leaves of Rhus chinensis

2017 ◽  
Vol 44 (12) ◽  
pp. 1160 ◽  
Author(s):  
Zhen-Yuan Ruan ◽  
Xiao-Ming Chen ◽  
Pu Yang ◽  
Bing-Yi Wang
Keyword(s):  
Cell Wall ◽  
Invertase Activity ◽  
Activity Level ◽  
Rapid Expansion ◽  
Sink Strength ◽  
Cell Wall Invertase ◽  
Vacuolar Invertase ◽  
Growing Period ◽  
Rhus Chinensis ◽  
Invertase Gene

The present study deals with the growth and development of the horn-shaped gall, which is induced by Schlechtendalia chinensis Bell. on leaves of Rhus chinensis Mill. The relationship between gall formers and their host plants was investigated by means of the activities of various invertases, the expressions of the cell wall invertase gene (INV2), and vacuolar invertase gene (INV3) during gall development. Our results show that the increase in the sink strength of the galls required cell wall invertase and vacuolar invertase, and that vacuolar invertase had a particular impact during the early development. In addition, vacuolar invertase activity was always significantly higher in galls than in leaves. However, ionically bound cell wall invertase showed a slightly significant increased activity level when compared with the leaves after galls had entered the fast growing period. This result indicates that vacuolar invertase is related to the rapid expansion of the galls, but ionically bound cell wall invertase is involved in the rapid growth of tissues. The enhanced activity of cell wall invertase and the expression of INV2 may be a plant response to a gall-induced stress. Cytoplasmic invertase that acts as a maintenance enzyme, or takes part in the production of secondary metabolites, was elevated when intracellular acid invertase activity decreased.

scholarly journals Effects of Cutting, Pruning, and Grafting on the Expression of Age-Related Genes in Larix kaempferi

Forests ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 218
Author(s):  
Yao Zhang ◽  
Qiao-Lu Zang ◽  
Li-Wang Qi ◽  
Su-Ying Han ◽  
Wan-Feng Li
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Regular Expression ◽  
Molecular Mechanisms ◽  
Expression Patterns ◽  
Reproductive Development ◽  
Larix Kaempferi ◽  
Clonal Forestry ◽  
Cdna Sequences ◽  
Age Related

Grafting, cutting, and pruning are important horticultural techniques widely used in the establishment of clonal forestry. After the application of these techniques, some properties of the plants change, however, the underlying molecular mechanisms are still unclear. In our previous study, 27 age-related transcripts were found to be expressed differentially between the juvenile vegetative (1- and 2-year-old) and adult reproductive (25- and 50-year-old) phases of Larix kaempferi. Here, we re-analyzed the 27 age-related transcripts, cloned their full-length cDNA sequences, and measured their responses to grafting, cutting, and pruning. After sequence analysis and cloning, 20 transcription factors were obtained and annotated, most of which were associated with reproductive development, and six (LaAGL2-1, LaAGL2-2, LaAGL2-3, LaSOC1-1, LaAGL11, and LaAP2-2) showed regular expression patterns with L. kaempferi aging. Based on the expression patterns of these transcription factors in L. kaempferi trees subjected to grafting, cutting, and pruning, we concluded that (1) cutting and pruning rejuvenate the plants and change their expression, and the effects of cutting on gene expression are detectable within 14 years, although the cutting seedlings are still maturing during these years; (2) within three months after grafting, the rootstock is more sensitive to grafting than the scion and readily becomes mature with the effect of the scion, while the scion is not readily rejuvenated by the effect of the rootstock; and (3) LaAGL2-2 and LaAGL2-3 are more sensitive to grafting, while LaAP2-2 is impervious to it. These findings not only provide potential molecular markers to assess the state of plants but also aid in studies of the molecular mechanisms of rejuvenation.

Isolation, Characterization and Promoter Analysis of Cell Wall Invertase Gene SoCIN1 from Sugarcane (Saccharum spp.)

Sugar Tech ◽  
2014 ◽  
Vol 17 (1) ◽  
pp. 65-76 ◽  
Author(s):  
Jun-Qi Niu ◽  
Ai-Qin Wang ◽  
Jing-Li Huang ◽  
Li-Tao Yang ◽  
Yang-Rui Li
Keyword(s):  
Cell Wall ◽  
Promoter Analysis ◽  
Cell Wall Invertase ◽  
Saccharum Spp ◽  
Invertase Gene

scholarly journals Direct Target Network of the Neurospora crassa Plant Cell Wall Deconstruction Regulators CLR-1, CLR-2, and XLR-1

mBio ◽  
2015 ◽  
Vol 6 (5) ◽  
Author(s):  
James P. Craig ◽  
Samuel T. Coradetti ◽  
Trevor L. Starr ◽  
N. Louise Glass
Keyword(s):  
Gene Expression ◽  
Cell Wall ◽  
Transcription Factors ◽  
Neurospora Crassa ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Nutrient Sensing ◽  
Wall Material ◽  
Content Type ◽  
Genes Encoding

ABSTRACTFungal deconstruction of the plant cell requires a complex orchestration of a wide array of intracellular and extracellular enzymes. InNeurospora crassa, CLR-1, CLR-2, and XLR-1 have been identified as key transcription factors regulating plant cell wall degradation in response to soluble sugars. The XLR-1 regulon was defined using a constitutively active mutant allele, resulting in hemicellulase gene expression and secretion under noninducing conditions. To define genes directly regulated by CLR-1, CLR-2, and XLR-1, we performed chromatin immunoprecipitation and next-generation sequencing (ChIPseq) on epitope-tagged constructs of these three transcription factors. WhenN. crassais exposed to plant cell wall material, CLR-1, CLR-2, and XLR-1 individually bind to the promoters of the most strongly induced genes in their respective regulons. These include promoters of genes encoding cellulases for CLR-1 and CLR-2 (CLR-1/CLR-2) and promoters of genes encoding hemicellulases for XLR-1. CLR-1 bound to its regulon under noninducing conditions; however, this binding alone did not translate into gene expression and enzyme secretion. Motif analysis of the bound genes revealed conserved DNA binding motifs, with the CLR-2 motif matching that of its closest paralog inSaccharomyces cerevisiae, Gal4p. Coimmunoprecipitation studies showed that CLR-1 and CLR-2 act in a homocomplex but not as a CLR-1/CLR-2 heterocomplex.IMPORTANCEUnderstanding fungal regulation of complex plant cell wall deconstruction pathways in response to multiple environmental signals via interconnected transcriptional circuits provides insight into fungus/plant interactions and eukaryotic nutrient sensing. Coordinated optimization of these regulatory networks is likely required for optimal microbial enzyme production.

Cloning and Analysis of MeCWINV6 Promoter from Biofuel Plant Cassava (Manihot esculenta Crantz)

2014 ◽  
Vol 986-987 ◽  
pp. 25-29
Author(s):  
Jiao Liu ◽  
Wen Rui Xia ◽  
Yan Ping Hu ◽  
Yuan Yao ◽  
Shao Ping Fu ◽  
...  
Keyword(s):  
Cell Wall ◽  
Pcr Amplification ◽  
Promoter Sequence ◽  
Starch Accumulation ◽  
Cell Wall Invertase ◽  
Potential Promoter ◽  
Invertase Gene ◽  
Responsive Element ◽  
Source Sink ◽  
Insight Into

In order to gain insight into the specific function of the cassava cell wall invertase 6 (MeCWINV6), the promoter sequence of MeCWINV6 gene was cloned using the PCR amplification approach. 118 bp CDS sequence and 1042 bp potential promoter sequence of MeCWINV6 gene were obtained. PlantCARE analyzed the putative cis-elements in silico revealed that these elements can be grouped into five classes: basic transcription elements (CAAT box and TATA box), light responsive elements (ACE, AE-box, ATCT-motif, AT1-motif, Box 4, GAG-motif, GT1-motif and Sp1), phytohormone responsive motifs (GARE-motif, TATC-box, TGACG-motif and TCA-element), defense and stress responsive element (TC-rich repeats and HSE), wounding and pathogen responsive elements (W-box and WUN-motif). This data demonstrate that it might be associated to regulate the cell wall invertase gene function in source-sink relations of cassava starch accumulation and response to internal and environmental stimuli.

Cold-induced repression of the rice anther-specific cell wall invertase gene OSINV4 is correlated with sucrose accumulation and pollen sterility

2005 ◽  
Vol 28 (12) ◽  
pp. 1534-1551 ◽  
Author(s):  
SANDRA N. OLIVER ◽  
JOOST T. VAN DONGEN ◽  
SANJEEV C. ALFRED ◽  
EZAZ A. MAMUN ◽  
XIAOCHUN ZHAO ◽  
...  
Keyword(s):  
Cell Wall ◽  
Pollen Sterility ◽  
Specific Cell ◽  
Sucrose Accumulation ◽  
Cell Wall Invertase ◽  
Invertase Gene

Molecular cloning and expression analysis of the cell-wall invertase gene family in rice (Oryza sativa L.)

2005 ◽  
Vol 24 (4) ◽  
pp. 225-236 ◽  
Author(s):  
Jung-Il Cho ◽  
Sang-Kyu Lee ◽  
Seho Ko ◽  
He-Kyung Kim ◽  
Sung-Hoon Jun ◽  
...  
Keyword(s):  
Cell Wall ◽  
Oryza Sativa ◽  
Gene Family ◽  
Molecular Cloning ◽  
Expression Analysis ◽  
Oryza Sativa L ◽  
Cloning And Expression ◽  
Cell Wall Invertase ◽  
Invertase Gene

Manipulating the expression of a cell wall invertase gene increases grain yield in maize

2017 ◽  
Vol 84 (1) ◽  
pp. 37-43 ◽  
Author(s):  
Yu-Jing Bi ◽  
Zhen-Cang Sun ◽  
Jessie Zhang ◽  
En-Qi Liu ◽  
Han-Mei Shen ◽  
...  
Keyword(s):  
Cell Wall ◽  
Grain Yield ◽  
Cell Wall Invertase ◽  
A Cell ◽  
Invertase Gene
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