scholarly journals Dual‐color 3D‐dSTORM colocalization and quantification of ROXY1 and RNAPII variants throughout the transcription cycle in root meristem nuclei

2020 ◽  
Vol 104 (5) ◽  
pp. 1423-1436
Author(s):  
Lucia Maß ◽  
Michael Holtmannspötter ◽  
Sabine Zachgo
Keyword(s):  
Root Meristem ◽  
Dual Color ◽  
Transcription Cycle

Ultrastructural Changes in the Root Meristem Cells of Rubus Chamaemorus L. (Cloudberry)

1975 ◽  
Vol 33 ◽  
pp. 562-563
Author(s):  
Arya K. Bal
Keyword(s):  
Root Meristem ◽  
Uranyl Acetate ◽  
Particulate Material ◽  
Ultrastructural Changes ◽  
Cell Organelles ◽  
Cytoplasmic Matrix ◽  
Golgi Membranes ◽  
Rubus Chamaemorus ◽  
Dense Substance ◽  
Ultrathin Sections

In the course of studies in the root meristem tissue of Rubus chamaemorus L. some important changes in the ultrastructural morphology were observed during the initiation of senescence at the end of the growing season.Root meristems were collected from naturally growing healthy populations of Cloudberry plants, and fixed in Karnovsky's mixture or in 2.5% glutaraldehyde in phosphate buffer. The samples were osmicated, dehydrated following usual methods and embedded in Epon. Ultrathin sections were stained in uranyl acetate and lead citrate.Figure 1 shows part of a dense cell in the meristem. The electron density of these cells is due to large amounts of a particulate material in the cytoplasmic matrix. The smallest particle seen in electron micrographs is about 40 A, although larger aggregates are also found, which remain randomly distributed in association with various cell organelles. Dense substance has been found associated with golgi membranes, proplastids, vacuoles and microtubules (Fig. 2).

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