scholarly journals Gene silencing of BnaA09.ZEP and BnaC09.ZEP confers orange color in Brassica napus flowers

2020 ◽  
Vol 104 (4) ◽  
pp. 932-949
Author(s):  
Yingjun Liu ◽  
Shenhua Ye ◽  
Gaigai Yuan ◽  
Xiaowei Ma ◽  
Shuangping Heng ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Gene Silencing ◽  
Orange Color

scholarly journals MAM gene silencing leads to the induction of C3 and reduction of C4 and C5 side-chain aliphatic glucosinolates in Brassica napus

2010 ◽  
Vol 27 (4) ◽  
pp. 467-478 ◽  
Author(s):  
Zheng Liu ◽  
Joe Hammerlindl ◽  
Wilf Keller ◽  
Peter B. E. McVetty ◽  
Fouad Daayf ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Gene Silencing ◽  
Side Chain ◽  
Aliphatic Glucosinolates

scholarly journals Assessing the Response of Small RNA Populations to Allopolyploidy Using Resynthesized Brassica napus Allotetraploids

2019 ◽  
Vol 36 (4) ◽  
pp. 709-726 ◽  
Author(s):  
Paulina Martinez Palacios ◽  
Marie-Pierre Jacquemot ◽  
Marion Tapie ◽  
Agnès Rousselet ◽  
Mamoudou Diop ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Gene Silencing ◽  
Genome Stability ◽  
Transcriptional Response ◽  
Early Response ◽  
Plant Evolution ◽  
Transcriptional Gene Silencing ◽  
Posttranscriptional Gene Silencing ◽  
Small Noncoding Rnas ◽  
Resynthesized Brassica Napus

Abstract Allopolyploidy, combining interspecific hybridization with whole genome duplication, has had significant impact on plant evolution. Its evolutionary success is related to the rapid and profound genome reorganizations that allow neoallopolyploids to form and adapt. Nevertheless, how neoallopolyploid genomes adapt to regulate their expression remains poorly understood. The hypothesis of a major role for small noncoding RNAs (sRNAs) in mediating the transcriptional response of neoallopolyploid genomes has progressively emerged. Generally, 21-nt sRNAs mediate posttranscriptional gene silencing by mRNA cleavage, whereas 24-nt sRNAs repress transcription (transcriptional gene silencing) through epigenetic modifications. Here, we characterize the global response of sRNAs to allopolyploidy in Brassica, using three independently resynthesized Brassica napus allotetraploids originating from crosses between diploid Brassica oleracea and Brassica rapa accessions, surveyed at two different generations in comparison with their diploid progenitors. Our results suggest an immediate but transient response of specific sRNA populations to allopolyploidy. These sRNA populations mainly target noncoding components of the genome but also target the transcriptional regulation of genes involved in response to stresses and in metabolism; this suggests a broad role in adapting to allopolyploidy. We finally identify the early accumulation of both 21- and 24-nt sRNAs involved in regulating the same targets, supporting a posttranscriptional gene silencing to transcriptional gene silencing shift at the first stages of the neoallopolyploid formation. We propose that reorganization of sRNA production is an early response to allopolyploidy in order to control the transcriptional reactivation of various noncoding elements and stress-related genes, thus ensuring genome stability during the first steps of neoallopolyploid formation.

scholarly journals Gene Silencing of BnTT10 Family Genes Causes Retarded Pigmentation and Lignin Reduction in the Seed Coat of Brassica napus

PLoS ONE ◽  
2013 ◽  
Vol 8 (4) ◽  
pp. e61247 ◽  
Author(s):  
Kai Zhang ◽  
Kun Lu ◽  
Cunmin Qu ◽  
Ying Liang ◽  
Rui Wang ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Gene Silencing ◽  
Seed Coat

scholarly journals Host Induced Gene Silencing of the Sclerotinia sclerotiorum ABHYDROLASE-3 gene reduces disease severity in Brassica napus

2021 ◽  
Author(s):  
Nick Wytinck ◽  
Dylan J Ziegler ◽  
Philip L Walker ◽  
Daniel S Sullivan ◽  
Kirsten T Biggar ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Gene Silencing ◽  
Sclerotinia Sclerotiorum ◽  
Fungal Pathogens ◽  
Pathogenic Fungus ◽  
Effective Means ◽  
Control Measures ◽  
Structural Barriers ◽  
Crop Species ◽  
Development Of Resistance

Sclerotinia sclerotiorum  is a pathogenic fungus that infects hundreds of crop species, causing extensive yield loss every year. Chemical fungicides are used to control this phytopathogen, but with concerns about increasing resistance and impacts on non-target species, there is a need to develop alternative control measures. In the present study, we engineered  Brassica napus  to constitutively express a hairpin (hp)RNA molecule to silence  ABHYRDOLASE-3  in  S. sclerotiorum . We demonstrate the potential for Host Induced Gene Silencing (HIGS) to protect  B. napus  from  S. sclerotiorum  using leaf, stem and whole plant infection assays. The interaction between the transgenic host plant and invading pathogen was further characterized at the molecular level using dual-RNA sequencing and at the anatomical level through microscopy to understand the processes and possible mechanisms leading to increased tolerance to this damaging necrotroph. We observed significant shifts in the expression of genes relating to plant defense as well as cellular differences in the form of structural barriers around the site of infection in the HIGS-protected plants. Our results provide proof-of-concept that HIGS is an effective means of limiting damage caused by  S. sclerotiorum  to the plant and demonstrates the utility of this biotechnology in the development of resistance against fungal pathogens.

968: Gelsolin Gene Silencing Involving Unusual Chromatin Structures and a Novel Stem Loop Structure of the Promoter Region in Human Bladder Cancer

2004 ◽  
Vol 171 (4S) ◽  
pp. 256-257
Author(s):  
Kazunori Haga ◽  
Ataru Sazawa ◽  
Toru Harabayashi ◽  
Nobuo Shinohara ◽  
Minoru Nomoto ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Gene Silencing ◽  
Promoter Region ◽  
Loop Structure ◽  
Human Bladder Cancer ◽  
Human Bladder ◽  
Stem Loop ◽  
Stem Loop Structure
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