scholarly journals NaTrxh is an essential protein for pollen rejection in Nicotiana by increasing S‐RNase activity

2020 ◽  
Vol 103 (4) ◽  
pp. 1304-1317 ◽  
Author(s):  
Maria D. Torres‐Rodríguez ◽  
Yuridia Cruz‐Zamora ◽  
Javier A. Juárez‐Díaz ◽  
Brian Mooney ◽  
Bruce A. McClure ◽  
...  
Keyword(s):  
Rnase Activity ◽  
Essential Protein ◽  
Pollen Rejection

scholarly journals STUDIES ON THE FUNCTION OF INTRACELLULAR RIBONUCLEASES

1966 ◽  
Vol 29 (3) ◽  
pp. 395-403 ◽  
Author(s):  
Takeshi Utsunomiya ◽  
Jay S. Roth
Keyword(s):  
Natural Products ◽  
Sodium Chloride ◽  
Rat Liver ◽  
Messenger Rna ◽  
Rnase Activity ◽  
Optimum Ph ◽  
Normal Rat ◽  
Pancreatic Rnase ◽  
The Stability ◽  
Normal Constituent

The RNase activity and properties of ribosome and polysome preparations from normal rat liver and some hepatomas have been examined. Polysome and ribosome preparations from the Novikoff, McCoy MDAB, and Dunning hepatomas had considerably higher specific RNase activity than corresponding preparations from normal rat liver, Novikoff ascites, or Morris 5123 hepatomas. The optimum pH of the RNase was approximately 8.5 for all samples tested, and the samples showed no evidence of latent RNase activity when treated with 3 M sodium chloride, EDTA, urea, or p-chloromercuribenzenesulfonic acid. The RNase activity appeared to be associated principally with breakdown products and/or subunits smaller than 80S. In the presence of Mg++ ions, subunits could reaggregate to form monomer ribosomes indistinguishable from the natural products, but some of the reassociated ribosomes could contain RNase activity which had been bound to the smaller particles. Similar results were obtained with spermine. In the hepatomas, evidence was obtained for the preexistence of considerable amounts of the smaller, RNase-containing subunits in the cell. When a small amount of crystalline bovine pancreatic RNase was added to partly dissociated ribosomes, the RNase was found only in association with the smaller subunits, and little or no enzyme was taken up by ribosomes or polysomes. The results have led to the conclusion that RNase is not a normal constituent of the ribosome or polysome, but that RNase may become associated with these particulates if dissociation and reassociation take place. Some implications of these findings for the stability of messenger RNA and for the mechanism of its breakdown are discussed.

Nob1p, a new essential protein, associates with the 26S proteasome of growing Saccharomyces cerevisiae cells

Gene ◽  
2000 ◽  
Vol 243 (1-2) ◽  
pp. 37-45 ◽  
Author(s):  
Yoshiko Tone ◽  
Nobuyuki Tanahashi ◽  
Keiji Tanaka ◽  
Masahiro Fujimuro ◽  
Hideyoshi Yokosawa ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
26S Proteasome ◽  
Essential Protein

scholarly journals Two Loci Contribute Epistastically to Heterospecific Pollen Rejection, a Postmating Isolating Barrier Between Species

2017 ◽  
Vol 7 (7) ◽  
pp. 2151-2159 ◽  
Author(s):  
Jennafer A P Hamlin ◽  
Natasha A Sherman ◽  
Leonie C Moyle
Keyword(s):  
Female Choice ◽  
Genetic Basis ◽  
Reproductive Tract ◽  
Interspecific Cross ◽  
Solanum Species ◽  
Female Reproductive Tract ◽  
Solanum Pennellii ◽  
Heterospecific Pollen ◽  
Male Gametes ◽  
Pollen Rejection

Abstract Recognition and rejection of heterospecific male gametes occurs in a broad range of taxa, although the complexity of mechanisms underlying these components of postmating cryptic female choice is poorly understood. In plants, the arena for postmating interactions is the female reproductive tract (pistil), within which heterospecific pollen tube growth can be arrested via active molecular recognition and rejection. Unilateral incompatibility (UI) is one such postmating barrier in which pollen arrest occurs in only one direction of an interspecific cross. We investigated the genetic basis of pistil-side UI between Solanum species, with the specific goal of understanding the role and magnitude of epistasis between UI QTL. Using heterospecific introgression lines (ILs) between Solanum pennellii and S. lycopersicum, we assessed the individual and pairwise effects of three chromosomal regions (ui1.1, ui3.1, and ui12.1) previously associated with interspecific UI among Solanum species. Specifically, we generated double introgression (‘pyramided’) genotypes that combined ui12.1 with each of ui1.1 and ui3.1, and assessed the strength of UI pollen rejection in the pyramided lines, compared to single introgression genotypes. We found that none of the three QTL individually showed UI rejection phenotypes, but lines combining ui3.1 and ui12.1 showed significant pistil-side pollen rejection. Furthermore, double ILs (DILs) that combined different chromosomal regions overlapping ui3.1 differed significantly in their rate of UI, consistent with at least two genetic factors on chromosome three contributing quantitatively to interspecific pollen rejection. Together, our data indicate that loci on both chromosomes 3 and 12 are jointly required for the expression of UI between S. pennellii and S. lycopersicum, suggesting that coordinated molecular interactions among a relatively few loci underlie the expression of this postmating prezygotic barrier. In addition, in conjunction with previous data, at least one of these loci appears to also contribute to conspecific self-incompatibility (SI), consistent with a partially shared genetic basis between inter- and intraspecific mechanisms of postmating prezygotic female choice.

scholarly journals S-RNase complexes and pollen rejection

2003 ◽  
Vol 54 (380) ◽  
pp. 123-130 ◽  
Author(s):  
F. Cruz-Garcia ◽  
C. N. Hancock ◽  
B. McClure
Keyword(s):  
Pollen Rejection

scholarly journals RNase Activity Prevents the Growth of a Fungal Pathogen in Tobacco Leaves and Increases upon Induction of Systemic Acquired Resistance with Elicitin

1997 ◽  
Vol 115 (4) ◽  
pp. 1557-1567 ◽  
Author(s):  
E. Galiana ◽  
P. Bonnet ◽  
S. Conrod ◽  
H. Keller ◽  
F. Panabieres ◽  
...  
Keyword(s):  
Fungal Pathogen ◽  
Systemic Acquired Resistance ◽  
Acquired Resistance ◽  
Rnase Activity ◽  
Tobacco Leaves
Sign in / Sign up

Export Citation Format

Share Document