scholarly journals Identification of an active miniature inverted‐repeat transposable element mJ ing in rice

2019 ◽  
Vol 98 (4) ◽  
pp. 639-653 ◽  
Author(s):  
Yanyan Tang ◽  
Xin Ma ◽  
Shuangshuang Zhao ◽  
Wei Xue ◽  
Xu Zheng ◽  
...  
Keyword(s):  
Transposable Element ◽  
Inverted Repeat

Miniature Inverted-Repeat Transposable Element Identification and Genetic Marker Development in Agrostis

Crop Science ◽  
2011 ◽  
Vol 51 (2) ◽  
pp. 854-861 ◽  
Author(s):  
Keenan Amundsen ◽  
David Rotter ◽  
Huaijun Michael Li ◽  
Joachim Messing ◽  
Geunhwa Jung ◽  
...  
Keyword(s):  
Transposable Element ◽  
Genetic Marker ◽  
Inverted Repeat ◽  
Marker Development

scholarly journals Genome-Wide Comparative Analysis of 20 Miniature Inverted-Repeat Transposable Element Families in Brassica rapa and B. oleracea

PLoS ONE ◽  
2014 ◽  
Vol 9 (4) ◽  
pp. e94499 ◽  
Author(s):  
Perumal Sampath ◽  
Jayakodi Murukarthick ◽  
Nur Kholilatul Izzah ◽  
Jonghoon Lee ◽  
Hong-Il Choi ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Transposable Element ◽  
Brassica Rapa ◽  
Inverted Repeat ◽  
Genome Wide

scholarly journals Translational repression by a miniature inverted-repeat transposable element in the 3′ untranslated region

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Jianqiang Shen ◽  
Juhong Liu ◽  
Kabin Xie ◽  
Feng Xing ◽  
Fang Xiong ◽  
...  
Keyword(s):  
Transposable Element ◽  
Inverted Repeat ◽  
Untranslated Region ◽  
Translational Repression

scholarly journals Unique Features of Aeromonas Plasmid pAC3 and Expression of the Plasmid-Mediated Quinolone Resistance Genes

mSphere ◽  
2017 ◽  
Vol 2 (3) ◽  
Author(s):  
Dae-Wi Kim ◽  
Cung Nawl Thawng ◽  
Sang Hee Lee ◽  
Chang-Jun Cha
Keyword(s):  
Transposable Element ◽  
Resistance Genes ◽  
Inverted Repeat ◽  
Mobile Element ◽  
Quinolone Resistance ◽  
Resistant Isolate ◽  
Fluoroquinolone Resistance ◽  
Content Type ◽  
E Coli ◽  
Resistance Determinants

ABSTRACT In the present study, plasmid pAC3 isolated from a highly fluoroquinolone-resistant isolate of Aeromonas species was sequenced and found to contain two fluoroquinolone resistance genes, aac(6′)-Ib-cr and qnrS2. Comparative analyses of plasmid pAC3 and other Aeromonas sp. IncU-type plasmids revealed a mobile insertion cassette element with a unique structure containing a qnrS2 gene and a typical miniature inverted-repeat transposable element (MITE) structure. This study also revealed that this MITE sequence appears in other Aeromonas species plasmids and chromosomes. Our results also demonstrate that the fluoroquinolone-dependent expression of qnrS2 is associated with rsd in E. coli DH5α harboring plasmid pAC3. Our findings suggest that the mobile element may play an important role in qnrS2 dissemination and that Aeromonas species constitute an important reservoir of fluoroquinolone resistance determinants in the environment. A highly fluoroquinolone-resistant isolate of Aeromonas species was isolated from a wastewater treatment plant and found to possess multiple resistance mechanisms, including mutations in gyrA and parC, efflux pumps, and plasmid-mediated quinolone resistance (PMQR) genes. Complete sequencing of the IncU-type plasmid, pAC3, present in the strain revealed a circular plasmid DNA 15,872 bp long containing two PMQR genes [qnrS2 and aac(6′)-Ib-cr]. A mobile insertion cassette element containing the qnrS2 gene and a typical miniature inverted-repeat transposable element (MITE) structure was identified in the plasmid. The present study revealed that this MITE sequence appears in other Aeromonas species plasmids and chromosomes. Plasmid pAC3 was introduced into Escherichia coli, and its PMQR genes were expressed, resulting in the acquisition of resistance. Proteome analysis of the recipient E. coli strain harboring the plasmid revealed that aac(6′)-Ib-cr expression was constitutive and that qnrS2 expression was dependent upon fluoroquinolone stress through regulation by regulator of sigma D (Rsd). To the best of our knowledge, this is the first report to characterize a novel MITE sequence upstream of the PMQR gene within a mobile insertion cassette, as well as the regulation of qnrS2 expression. Our results suggest that this mobile element may play an important role in qnrS2 dissemination. IMPORTANCE In the present study, plasmid pAC3 isolated from a highly fluoroquinolone-resistant isolate of Aeromonas species was sequenced and found to contain two fluoroquinolone resistance genes, aac(6′)-Ib-cr and qnrS2. Comparative analyses of plasmid pAC3 and other Aeromonas sp. IncU-type plasmids revealed a mobile insertion cassette element with a unique structure containing a qnrS2 gene and a typical miniature inverted-repeat transposable element (MITE) structure. This study also revealed that this MITE sequence appears in other Aeromonas species plasmids and chromosomes. Our results also demonstrate that the fluoroquinolone-dependent expression of qnrS2 is associated with rsd in E. coli DH5α harboring plasmid pAC3. Our findings suggest that the mobile element may play an important role in qnrS2 dissemination and that Aeromonas species constitute an important reservoir of fluoroquinolone resistance determinants in the environment.

scholarly journals A miniature inverted-repeat transposable element, AddIn-MITE, located inside a WD40 gene is conserved in Andropogoneae grasses

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6080
Author(s):  
Clicia Grativol ◽  
Flavia Thiebaut ◽  
Sara Sangi ◽  
Patricia Montessoro ◽  
Walaci da Silva Santos ◽  
...  
Keyword(s):  
Transposable Elements ◽  
Transposable Element ◽  
Small Rna ◽  
Genome Assembly ◽  
Inverted Repeat ◽  
Small Rnas ◽  
Distribution Patterns ◽  
Plant Genomes ◽  
Sugarcane Genome

Miniature inverted-repeat transposable elements (MITEs) have been associated with genic regions in plant genomes and may play important roles in the regulation of nearby genes via recruitment of small RNAs (sRNA) to the MITEs loci. We identified eight families of MITEs in the sugarcane genome assembly with MITE-Hunter pipeline. These sequences were found to be upstream, downstream or inserted into 67 genic regions in the genome. The position of the most abundant MITE (Stowaway-like) in genic regions, which we call AddIn-MITE, was confirmed in a WD40 gene. The analysis of four monocot species showed conservation of the AddIn-MITE sequence, with a large number of copies in their genomes. We also investigated the conservation of the AddIn-MITE’ position in the WD40 genes from sorghum, maize and, in sugarcane cultivars and wild Saccharum species. In all analyzed plants, AddIn-MITE has located in WD40 intronic region. Furthermore, the role of AddIn-MITE-related sRNA in WD40 genic region was investigated. We found sRNAs preferentially mapped to the AddIn-MITE than to other regions in the WD40 gene in sugarcane. In addition, the analysis of the small RNA distribution patterns in the WD40 gene and the structure of AddIn-MITE, suggests that the MITE region is a proto-miRNA locus in sugarcane. Together, these data provide insights into the AddIn-MITE role in Andropogoneae grasses.

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