scholarly journals A repertoire of cationic and anionic conductances at the plasma membrane of Medicago truncatula root hairs

2019 ◽  
Vol 98 (3) ◽  
pp. 418-433 ◽  
Author(s):  
Limin Wang ◽  
Man‐Yuan Guo ◽  
Jean‐Baptiste Thibaud ◽  
Anne‐Aliénor Véry ◽  
Hervé Sentenac
Keyword(s):  
Plasma Membrane ◽  
Medicago Truncatula ◽  
Root Hairs

scholarly journals Formin-mediated bridging of cell wall, plasma membrane, and cytoskeleton in symbiotic infections of Medicago truncatula

2021 ◽  
Author(s):  
Pengbo Liang ◽  
Clara Schmitz ◽  
Beatrice Lace ◽  
Franck Anicet Ditengou ◽  
Chao Su ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plasma Membrane ◽  
Medicago Truncatula

scholarly journals Heterologous Expression of Rhizobial CelC2 Cellulase Impairs Symbiotic Signaling and Nodulation in Medicago truncatula

2018 ◽  
Vol 31 (5) ◽  
pp. 568-575 ◽  
Author(s):  
Marta Robledo ◽  
Esther Menéndez ◽  
Jose Ignacio Jiménez-Zurdo ◽  
Raúl Rivas ◽  
Encarna Velázquez ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Cell Walls ◽  
Rhizobium Leguminosarum ◽  
Root Nodules ◽  
Root Hairs ◽  
Nod Factors ◽  
Model Legume ◽  
Ensifer Meliloti ◽  
Early Nodulin ◽  
The Impact

The infection of legume plants by rhizobia is tightly regulated to ensure accurate bacterial penetration, infection, and development of functionally efficient nitrogen-fixing root nodules. Rhizobial Nod factors (NF) have key roles in the elicitation of nodulation signaling. Infection of white clover roots also involves the tightly regulated specific breakdown of the noncrystalline apex of cell walls in growing root hairs, which is mediated by Rhizobium leguminosarum bv. trifolii cellulase CelC2. Here, we have analyzed the impact of this endoglucanase on symbiotic signaling in the model legume Medicago truncatula. Ensifer meliloti constitutively expressing celC gene exhibited delayed nodulation and elicited aberrant ineffective nodules, hampering plant growth in the absence of nitrogen. Cotreatment of roots with NF and CelC2 altered Ca2+ spiking in root hairs and induction of the early nodulin gene ENOD11. Our data suggest that CelC2 alters early signaling between partners in the rhizobia-legume interaction.

Recycling domains in plant cell morphogenesis: small GTPase effectors, plasma membrane signalling and the exocyst

2010 ◽  
Vol 38 (2) ◽  
pp. 723-728 ◽  
Author(s):  
Viktor Žárský ◽  
Martin Potocký
Keyword(s):  
Plasma Membrane ◽  
Plant Cell ◽  
Secretory Pathway ◽  
Root Hairs ◽  
Small Gtpases ◽  
Small Gtpase ◽  
Cell Cortex ◽  
Membrane Phospholipids ◽  
Recycling Endosome ◽  
Regulatory Module

The Rho/Rop small GTPase regulatory module is central for initiating exocytotically ACDs (active cortical domains) in plant cell cortex, and a growing array of Rop regulators and effectors are being discovered in plants. Structural membrane phospholipids are important constituents of cells as well as signals, and phospholipid-modifying enzymes are well known effectors of small GTPases. We have shown that PLDs (phospholipases D) and their product, PA (phosphatidic acid), belong to the regulators of the secretory pathway in plants. We have also shown that specific NOXs (NADPH oxidases) producing ROS (reactive oxygen species) are involved in cell growth as exemplified by pollen tubes and root hairs. Most plant cells exhibit several distinct plasma membrane domains (ACDs), established and maintained by endocytosis/exocytosis-driven membrane protein recycling. We proposed recently the concept of a ‘recycling domain’ (RD), uniting the ACD and the connected endosomal recycling compartment (endosome), as a dynamic spatiotemporal entity. We have described a putative GTPase–effector complex exocyst involved in exocytic vesicle tethering in plants. Owing to the multiplicity of its Exo70 subunits, this complex, along with many RabA GTPases (putative recycling endosome organizers), may belong to core regulators of RD organization in plants.

scholarly journals The Small GTPase ROP10 of Medicago truncatula Is Required for Both Tip Growth of Root Hairs and Nod Factor-Induced Root Hair Deformation

2015 ◽  
Vol 27 (3) ◽  
pp. 806-822 ◽  
Author(s):  
Ming-Juan Lei ◽  
Qi Wang ◽  
Xiaolin Li ◽  
Aimin Chen ◽  
Li Luo ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Root Hair ◽  
Tip Growth ◽  
Root Hairs ◽  
Small Gtpase ◽  
Nod Factor ◽  
Root Hair Deformation

scholarly journals AtRBOHC/RHD2 vesicular delivery to the apical plasma membrane domain during root hair development

2021 ◽  
Author(s):  
Lenka Kuběnová ◽  
Michaela Tichá ◽  
Jozef Šamaj ◽  
Miroslav Ovečka
Keyword(s):  
Plasma Membrane ◽  
Quantitative Analysis ◽  
Root Hair ◽  
Tip Growth ◽  
Root Hairs ◽  
Apical Plasma Membrane ◽  
Loss Of Function ◽  
Short Root ◽  
Early Endosomes ◽  
Root Hair Initiation

AbstractArabidopsis root hairs develop as long tubular extensions from the rootward pole of trichoblasts and exert polarized tip growth. The establishment and maintenance of root hair polarity is a complex process involving the local apical production of reactive oxygen species (ROS) generated by NADPH oxidase RESPIRATORY BURST OXIDASE HOMOLOG PROTEIN C/ROOT HAIR DEFECTIVE 2 (AtRBOHC/RHD2). It has been shown that loss-of-function rhd2 mutants have short root hairs that are unable to elongate by tip growth, and this phenotype was fully complemented by GFP-RHD2 expressed under the RHD2 promoter. However, the spatiotemporal mechanism of AtRBOHC/RHD2 subcellular redistribution and delivery to the plasma membrane (PM) during root hair initiation and tip growth are still unclear. Here, we used advanced microscopy for detailed qualitative and quantitative analysis of vesicular compartments containing GFP-RHD2 and characterization of their movements in developing bulges and growing root hairs. These compartments, identified by an independent marker such as the trans-Golgi network (TGN), deliver GFP-RHD2 to the apical PM domain, the extent of which correlates with the stage of root hair formation. Movements of TGN/early endosomes, but not late endosomes, were affected in the bulging domains of the rhd2-1 mutant. Finally, we reveal that accumulation in the growing tip, docking, and incorporation of TGN compartments containing GFP-RHD2 to the apical PM of root hairs requires structural sterols. These results help clarify the mechanism of polarized AtRBOHC/RHD2 targeting, maintenance, and recycling at the apical PM domain, coordinated with different developmental stages of root hair initiation and growth.One-sentence summaryAdvanced microscopy and quantitative analysis of vesicular TGN compartments revealed that delivering GFP-RHD2 to the apical plasma membrane domains of developing bulges and growing root hairs requires structural sterols.

scholarly journals Roles of plasma membrane proton ATPases AHA2 and AHA7 in normal growth of roots and root hairs inArabidopsis thaliana

2018 ◽  
Vol 166 (3) ◽  
pp. 848-861 ◽  
Author(s):  
Robert D. Hoffmann ◽  
Lene I. Olsen ◽  
Chukwuebuka V. Ezike ◽  
Jesper T. Pedersen ◽  
Raffaele Manstretta ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Root Hairs ◽  
Normal Growth

scholarly journals Mechanism of Infection Thread Elongation in Root Hairs of Medicago truncatula and Dynamic Interplay with Associated Rhizobial Colonization

2008 ◽  
Vol 148 (4) ◽  
pp. 1985-1995 ◽  
Author(s):  
Joëlle Fournier ◽  
Antonius C.J. Timmers ◽  
Björn J. Sieberer ◽  
Alain Jauneau ◽  
Mireille Chabaud ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Root Hairs ◽  
Infection Thread ◽  
Dynamic Interplay
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