scholarly journals Involvement of chalcone reductase in the soybean isoflavone metabolon: identification of GmCHR5, which interacts with 2-hydroxyisoflavanone synthase

2018 ◽  
Vol 96 (1) ◽  
pp. 56-74 ◽  
Author(s):  
Ryo Mameda ◽  
Toshiyuki Waki ◽  
Yosuke Kawai ◽  
Seiji Takahashi ◽  
Toru Nakayama
Keyword(s):  
Soybean Isoflavone ◽  
Chalcone Reductase

Chalcone reductase-homologous transcripts accumulate during development of stem-borne nodules on the tropical legume Sesbania rostrata

Planta ◽  
1999 ◽  
Vol 209 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Sofie Goormachtig ◽  
Sam Lievens ◽  
Sylvia Herman ◽  
Marc Van Montagu ◽  
Marcelle Holsters
Keyword(s):  
Sesbania Rostrata ◽  
Tropical Legume ◽  
Chalcone Reductase

scholarly journals A Novel Thermostable GH3β-Glucosidase fromTalaromyce leycettanuswith Broad Substrate Specificity and Significant Soybean Isoflavone Glycosides-Hydrolyzing Capability

2018 ◽  
Vol 2018 ◽  
pp. 1-9
Author(s):  
Xinxin Li ◽  
Wei Xia ◽  
Yingguo Bai ◽  
Rui Ma ◽  
Hong Yang ◽  
...  
Keyword(s):  
Amino Acid ◽  
Glycoside Hydrolase ◽  
Animal Feed ◽  
Amino Acid Residues ◽  
Calculated Molecular Mass ◽  
Substrate Specificities ◽  
Soybean Isoflavone ◽  
Food Animal ◽  
Isoflavone Glycoside ◽  
Hydrolysis Rates

A novelβ-glucosidase gene (Bgl3B) of glycoside hydrolase (GH) family 3 was cloned from the thermophilic fungusTalaromyce leycettanusJM12802 and successfully expressed inPichia pastoris. The deduced Bgl3B contains 860 amino acid residues with a calculated molecular mass of 91.2 kDa. The purified recombinant Bgl3B exhibited maximum activities at pH 4.5 and 65°C and remained stable at temperatures up to 60°C and pH 3.0−9.0, respectively. The enzyme exhibited broad substrate specificities, showingβ-glucosidase, glucanase, cellobiase, xylanase, and isoflavone glycoside hydrolase activities, and its activities were stimulated by short-chain alcohols. The catalytic efficiencies of Bgl3B were 693 and 104/mM/s towardspNPG and cellobiose, respectively. Moreover, Bgl3B was highly effective in converting isoflavone glycosides to aglycones at 37°C within 10 min, with the hydrolysis rates of 95.1%, 76.0%, and 75.3% for daidzin, genistin, and glycitin, respectively. These superior properties make Bgl3B potential for applications in the food, animal feed, and biofuel industries.

The effects of dietary soybean isoflavone on immunity in Chinese yellow-feathered broilers challenged with infectious bursal disease virus

2018 ◽  
Vol 156 (6) ◽  
pp. 832-838 ◽  
Author(s):  
S. Q. Jiang ◽  
Z. Y. Jiang ◽  
J. L. Chen ◽  
C. Zhu ◽  
P. Hong ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
T Lymphocyte ◽  
Lymphocyte Proliferation ◽  
Infectious Bursal Disease Virus ◽  
Infectious Bursal Disease ◽  
Soybean Isoflavone ◽  
T Lymphocyte Proliferation ◽  
Serum Iga ◽  
Bursal Disease ◽  
Antibody Titres

AbstractTo investigate the effects of soybean isoflavone (SI) on immunity in infectious bursal disease virus (IBDV)-infected broilers, chicks were fed the same basal diet supplemented with 0 (non-infected control), 0 (infected control), 10, 20 or 40 mg/kg SI for 44 days. At 21 days old, chickens were inoculated with bursal infectious dose causing 50% morbidity of the IBDV BC 6/85 strain by the eye-drop and nasal route (except for non-infected controls). Results showed that, over 1–23 days post-infection (dpi), there was a significant interaction between SI supplementation level and time: high-level SI supplementation increased peripheral T lymphocyte proliferation, percentages of CD3+, CD4+and CD8+T lymphocytes, CD4+to CD8+ratio, serum concentrations of IgA, IgM and IgG, and IBDV antibody titres. Except for serum IgA and IgM, these variables increased over time with far higher values at 23 dpi than earlier. Compared with non-infected controls, IBDV inoculation decreased peripheral T lymphocyte proliferation at 3 dpi, percentages of CD3+, CD4+and CD8+T lymphocytes, and serum IgG, IgM concentration at 23 dpi, and increased IBDV antibody titres at 7, 15 and 23 dpi. Supplemental SI quadratically increased peripheral T lymphocyte proliferation, CD4+to CD8+ratio and serum IgA concentration at 3 dpi, percentages of CD3+, CD4+and CD8+T lymphocytes at 3 and 23 dpi, and serum IgM concentration and IBDV antibody titres at 23 dpi. These results indicate that dietary SI improved cellular and humoral immunity of IBDV-infected birds and may enhance resistance of Yellow-feathered broilers to infectious diseases.

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