scholarly journals Natural variation for gene expression responses to abiotic stress in maize

2017 ◽  
Vol 89 (4) ◽  
pp. 706-717 ◽  
Author(s):  
Amanda J. Waters ◽  
Irina Makarevitch ◽  
Jaclyn Noshay ◽  
Liana T. Burghardt ◽  
Candice N. Hirsch ◽  
...  
Keyword(s):  
Gene Expression ◽  
Abiotic Stress ◽  
Natural Variation

scholarly journals Natural Variation in Abiotic Stress Responsive Gene Expression and Local Adaptation to Climate in Arabidopsis thaliana

2014 ◽  
Vol 31 (9) ◽  
pp. 2283-2296 ◽  
Author(s):  
Jesse R. Lasky ◽  
David L. Des Marais ◽  
David B. Lowry ◽  
Inna Povolotskaya ◽  
John K. McKay ◽  
...  
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Abiotic Stress ◽  
Local Adaptation ◽  
Natural Variation ◽  
Responsive Gene

scholarly journals R2R3-MYB transcription factors, StmiR858 and sucrose mediate potato flavonol biosynthesis

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Sen Lin ◽  
Rajesh K. Singh ◽  
Moehninsi ◽  
Duroy A. Navarre
Keyword(s):  
Gene Expression ◽  
Abiotic Stress ◽  
Feedback Loop ◽  
Regulatory Mechanisms ◽  
Phenylpropanoid Metabolism ◽  
Biotic And Abiotic Stress ◽  
Health Promoting ◽  
Transient Overexpression ◽  
R2r3 Myb ◽  
R2r3 Myb Transcription Factors

AbstractFlavonols and other phenylpropanoids protect plants from biotic and abiotic stress and are dietarily desirable because of their health-promoting properties. The ability to develop new potatoes (Solanum tuberosum) with optimal types and amounts of phenylpropanoids is limited by lack of knowledge about the regulatory mechanisms. Exogenous sucrose increased flavonols, whereas overexpression of the MYB StAN1 induced sucrolytic gene expression. Heterologous StAN1 protein bound promoter fragments from sucrolytic genes (SUSY1 and INV1). Two additional MYBs and one microRNA were identified that regulated potato flavonols. Overexpression analysis showed MYB12A and C increased amounts of flavonols and other phenylpropanoids. Endogenous flavonol amounts in light-exposed organs were much higher those in the dark. Expression levels of StMYB12A and C were high in flowers but low in tubers. Transient overexpression of miR858 altered potato flavonol metabolism. Endogenous StmiR858 expression was much lower in flowers than leaves and correlated with flavonol amounts in these organs. Collectively, these findings support the hypothesis that sucrose, MYBs, and miRNA control potato phenylpropanoid metabolism in a finely tuned manner that includes a feedback loop between sucrose and StAN1. These findings will aid in the development of potatoes with phenylpropanoid profiles optimized for crop performance and human health.

scholarly journals Assessing the Genome-Wide Effect of Promoter Region Tandem Repeat Natural Variation on Gene Expression

2012 ◽  
Vol 2 (12) ◽  
pp. 1643-1649 ◽  
Author(s):  
Martha H. Elmore ◽  
John G. Gibbons ◽  
Antonis Rokas
Keyword(s):  
Gene Expression ◽  
Tandem Repeat ◽  
Promoter Region ◽  
Natural Variation ◽  
Genome Wide

scholarly journals Natural variation of DNA methylation and gene expression may determine local adaptations of Scots pine populations

2018 ◽  
Vol 69 (21) ◽  
pp. 5293-5305 ◽  
Author(s):  
Emmi Alakärppä ◽  
Heikki M Salo ◽  
Luis Valledor ◽  
Maria Jesús Cañal ◽  
Hely Häggman ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Scots Pine ◽  
Natural Variation ◽  
Local Adaptations

scholarly journals An ant–plant mutualism through the lens of cGMP-dependent kinase genes

2017 ◽  
Vol 284 (1862) ◽  
pp. 20170896 ◽  
Author(s):  
Pierre-Jean G. Malé ◽  
Kyle M. Turner ◽  
Manjima Doha ◽  
Ina Anreiter ◽  
Aaron M. Allen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Molecular Basis ◽  
Significant Interaction ◽  
Natural Variation ◽  
Foraging Behaviour ◽  
Dependent Protein Kinase ◽  
Ant Colonies ◽  
Cgmp Dependent Protein Kinase ◽  
Dependent Protein ◽  
Behaviour Changes

In plant–animal mutualisms, how an animal forages often determines how much benefit its plant partner receives. In many animals, foraging behaviour changes in response to foraging gene expression or activation of the cGMP-dependent protein kinase (PKG) that foraging encodes. Here, we show that this highly conserved molecular mechanism affects the outcome of a plant–animal mutualism. We studied the two PKG genes of Allomerus octoarticulatus, an Amazonian ant that defends the ant–plant Cordia nodosa against herbivores. Some ant colonies are better ‘bodyguards’ than others. Working in the field in Peru, we found that colonies fed with a PKG activator recruited more workers to attack herbivores than control colonies. This resulted in less herbivore damage. PKG gene expression in ant workers correlated with whether an ant colony discovered an herbivore and how much damage herbivores inflicted on leaves in a complex way; natural variation in expression levels of the two genes had significant interaction effects on ant behaviour and herbivory. Our results suggest a molecular basis for ant protection of plants in this mutualism.

scholarly journals Global gene expression patterns in response to white patch syndrome: Disentangling symbiont loss from the thermal stress response in reef-building coral

2019 ◽  
Author(s):  
Carly D. Kenkel ◽  
Veronique J.L. Mocellin ◽  
Line K. Bay
Keyword(s):  
Gene Expression ◽  
Abiotic Stress ◽  
Thermal Stress ◽  
Stress Response ◽  
Meta Analysis ◽  
Expression Patterns ◽  
Global Gene Expression ◽  
Host Protein ◽  
Gene Expression Patterns ◽  
White Patch

AbstractThe mechanisms resulting in the breakdown of the coral symbiosis once the process of bleaching has been initiated remain unclear. Distinguishing symbiont loss from the abiotic stress response may shed light on the cellular and molecular pathways involved in each process. This study examined physiological changes and global gene expression patterns associated with white patch syndrome (WPS) in P. lobata, which manifests in localized bleaching independent of thermal stress. In addition, a meta-analysis of global gene expression studies in other corals and anemones was used to contrast differential regulation as a result of abiotic stress from expression patterns correlated with symbiotic state. Symbiont density, chlorophyll a content, holobiont productivity, instant calcification rate, and total host protein content were uniformly reduced in WPS relative to healthy tissue. While expression patterns associated with WPS were secondary to fixed effects of source colony, specific functional enrichments suggest that the viral infection putatively giving rise to this condition affects symbiont rather than host cells. The meta-analysis revealed that expression patterns in WPS-affected tissues were significantly correlated with prior studies examining short-term thermal stress responses. This correlation was independent of symbiotic state, as the strongest correlations were found between WPS adults and both symbiotic adult and aposymbiotic coral larvae experiencing thermal stress, suggesting that the majority of expression changes reflect a non-specific stress response. Across studies, the magnitude and direction of expression change among particular functional enrichments suggests unique responses to stressor duration, and highlights unique responses to bleaching in an anemone model which engages in a non-obligate symbiosis.

scholarly journals RETRACTED ARTICLE: The specific MYB binding sites bound by TaMYB in the GAPCp2/3 promoters are involved in the drought stress response in wheat

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Lin Zhang ◽  
Zhiqiang Song ◽  
Fangfang Li ◽  
Xixi Li ◽  
Haikun Ji ◽  
...  
Keyword(s):  
Gene Expression ◽  
Abiotic Stress ◽  
Drought Stress ◽  
Reporter Gene ◽  
Binding Sites ◽  
Promoter Sequence ◽  
Regulatory Elements ◽  
Cis Elements ◽  
Gus Reporter ◽  
Almost All

Abstract Background Drought stress is one of the major abiotic stresses that affects plant growth and productivity. The GAPCp genes play important roles in drought stress tolerance in multiple species. The aim of this experiment was to identify the core cis-regulatory elements that may respond to drought stress in the GAPCp2 and GAPCp3 promoter sequences. Results In this study, the promoters of GAPCp2 and GAPCp3 were cloned. The promoter activities were significantly improved under abiotic stress via regulation of Rluc reporter gene expression, while promoter sequence analysis indicated that these fragments were not almost identical. In transgenic Arabidopsis with the expression of the GUS reporter gene under the control of one of these promoters, the activities of GUS were strong in almost all tissues except the seeds, and the activities were induced after abiotic stress. The yeast one-hybrid system and EMSA demonstrated that TaMYB bound TaGAPCp2P/3P. By analyzing different 5′ deletion mutants of these promoters, it was determined that TaGAPCp2P (− 1312~ − 528) and TaGAPCp3P (− 2049~ − 610), including the MYB binding site, contained enhancer elements that increased gene expression levels under drought stress. We used an effector and a reporter to co-transform tobacco and found that TaMYB interacted with the specific MYB binding sites of TaGAPCp2P (− 1197~ − 635) and TaGAPCp3P (− 1456~ − 1144 and − 718~ − 610) in plant cells. Then, the Y1H system and EMSA assay demonstrated that these MYB binding sites in TaGAPCp2P (− 1135 and − 985) and TaGAPCp3P (− 1414 and − 665) were the target cis-elements of TaMYB. The deletion of the specific MYB binding sites in the promoter fragments significantly restrained the drought response, and these results confirmed that these MYB binding sites (AACTAAA/C) play vital roles in improving the transcription levels under drought stress. The results of qRT-PCR in wheat protoplasts transiently overexpressing TaMYB indicated that the expression of TaGAPCp2/3 induced by abiotic stress was upregulated by TaMYB. Conclusion The MYB binding sites (AACTAAA/C) in TaGAPCp2P/3P were identified as the key cis-elements for responding to drought stress and were bound by the transcription factor TaMYB.

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