scholarly journals Mapping-by-sequencing in complex polyploid genomes using genic sequence capture: a case study to map yellow rust resistance in hexaploid wheat

2016 ◽  
Vol 87 (4) ◽  
pp. 403-419 ◽  
Author(s):  
Laura-Jayne Gardiner ◽  
Pauline Bansept-Basler ◽  
Lisa Olohan ◽  
Ryan Joynson ◽  
Rachel Brenchley ◽  
...  
Keyword(s):  
Hexaploid Wheat ◽  
Rust Resistance ◽  
Yellow Rust ◽  
Sequence Capture ◽  
Yellow Rust Resistance ◽  
Mapping By Sequencing

scholarly journals A framework for gene mapping in wheat demonstrated using the Yr7 yellow rust resistance gene

2019 ◽  
Author(s):  
Laura-Jayne Gardiner ◽  
Pauline Bansept-Basler ◽  
Mohamed El-Soda ◽  
Anthony Hall ◽  
Donal M. O’Sullivan
Keyword(s):  
Association Mapping ◽  
Candidate Gene ◽  
Resistance Gene ◽  
Rust Resistance ◽  
Yellow Rust ◽  
Snp Array ◽  
Rust Resistance Gene ◽  
Mapping Panel ◽  
Yellow Rust Resistance ◽  
Mapping By Sequencing

AbstractWe used three approaches to map the yellow rust resistance gene Yr7 and identify associated SNPs in wheat. First, we used a traditional QTL mapping approach using a double haploid (DH) population and mapped Yr7 to a low-recombination region of chromosome 2B. To fine map the QTL, we then used an association mapping panel. Both populations were SNP array genotyped allowing alignment of QTL and genome-wide association scans based on common segregating SNPs. Analysis of the association panel spanning the QTL interval, narrowed the interval down to a single haplotype block. Finally, we used mapping-by-sequencing of resistant and susceptible DH bulks to identify a candidate gene in the interval showing high homology to a previously suggested Yr7 candidate and to populate the Yr7 interval with a higher density of polymorphisms. We highlight the power of combining mapping-by-sequencing, delivering a complete list of gene-based segregating polymorphisms in the interval with the high recombination, low LD precision of the association mapping panel. Our mapping-by-sequencing methodology is applicable to any trait and our results validate the approach in wheat, where with a near complete reference genome sequence, we are able to define a small interval containing the causative gene.HighlightWe show progression from genetic mapping to mapping-by-sequencing and the overlap of defined intervals by each approach culminating with interval refinement and identification of a candidate gene for disease resistance.

Clustering of SSR markers located on 2AL for yellow rust resistance in hexaploid wheat (Triticum aestivum L.)

2012 ◽  
Vol 29 ◽  
pp. S179
Author(s):  
Funda Senturk Akfirat ◽  
Fahriye Ertugrul ◽  
Mehmet Cakir ◽  
Semra Hasancebi ◽  
Yildiz Aydin ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Ssr Markers ◽  
Hexaploid Wheat ◽  
Rust Resistance ◽  
Yellow Rust ◽  
Triticum Aestivum L ◽  
Yellow Rust Resistance

An EST-SSR marker linked with yellow rust resistance in wheat

2010 ◽  
Vol 54 (4) ◽  
pp. 691-696 ◽  
Author(s):  
S. Ercan ◽  
F. Ertugrul ◽  
Y. Aydin ◽  
F. S. Akfirat ◽  
S. Hasancebi ◽  
...  
Keyword(s):  
Rust Resistance ◽  
Ssr Marker ◽  
Yellow Rust ◽  
Yellow Rust Resistance

scholarly journals Identification of an AFLP marker linked with yellow rust resistance in wheat (Triticum aestivum L.)

2014 ◽  
Vol 38 ◽  
pp. 371-379 ◽  
Author(s):  
Handan BALTA ◽  
Özge KARAKAŞ METİN ◽  
Funda ŞENTÜRK AKFIRAT ◽  
Fahriye ERTUĞRUL ◽  
Semra HASANÇEBİ ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Rust Resistance ◽  
Aflp Marker ◽  
Yellow Rust ◽  
Triticum Aestivum L ◽  
Yellow Rust Resistance
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