scholarly journals OsNAC2encoding a NAC transcription factor that affects plant height through mediating the gibberellic acid pathway in rice

2015 ◽  
Vol 82 (2) ◽  
pp. 302-314 ◽  
Author(s):  
Xu Chen ◽  
Songchong Lu ◽  
Yaofeng Wang ◽  
Xuan Zhang ◽  
Bo Lv ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Gibberellic Acid ◽  
Plant Height ◽  
Nac Transcription Factor ◽  
Acid Pathway

GRAS transcription factor LOSS OF AXILLARY MERISTEMS is essential for stamen and runner formation in wild strawberry

2021 ◽  
Author(s):  
Jia Feng ◽  
Laichao Cheng ◽  
Zhenying Zhu ◽  
Feiqi Yu ◽  
Cheng Dai ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Gibberellic Acid ◽  
Complementation Test ◽  
Causative Mutation ◽  
Gras Transcription Factor ◽  
Axillary Meristems ◽  
Wild Strawberry ◽  
Acid Pathway ◽  
Bud Initiation ◽  
Branch Crown

Abstract Axillary bud development is a major factor that impacts plant architecture. A runner is an elongated shoot that develops from axillary buds and is frequently used for clonal propagation of strawberry. However, the genetic control underlying runner production is largely unknown. Here, we identified and characterized loss of axillary meristems (lam), an EMS-induced mutant of the diploid woodland strawberry (Fragaria vesca) that lacked stamens in flowers and had reduced numbers of branch crowns and runners. The reduced branch crown and runner phenotypes were caused by a failure of axillary meristem initiation. The causative mutation of lam was located in FvH4_3g41310, which encodes a GRAS transcription factor, and was validated by a complementation test. lamCR mutants generated by CRISPR/Cas9 produced flowers without stamens and had fewer runners than the wild type. LAM was broadly expressed in meristematic tissues. Gibberellic acid (GA) application induced runner outgrowth from the remaining buds in lam, but failed to do so at the empty axils of lam. In contrast, treatment with the GA biosynthesis inhibitor paclobutrazol (PBZ) converted the runners into branch crowns. Moreover, genetic studies indicated that lam is epistatic to suppressor of runnerless (srl), a mutant of FveRGA1 in the gibberellic acid pathway, during runner formation. Our results demonstrate that LAM is required for stamen and runner formation and acts sequentially with GA from bud initiation to runner outgrowth, providing insights into the molecular regulation of these economically important organs in strawberry.

scholarly journals NAC Transcription Factor PwNAC11 Activates ERD1 by Interaction with ABF3 and DREB2A to Enhance Drought Tolerance in Transgenic Arabidopsis

2021 ◽  
Vol 22 (13) ◽  
pp. 6952
Author(s):  
Mingxin Yu ◽  
Junling Liu ◽  
Bingshuai Du ◽  
Mengjuan Zhang ◽  
Aibin Wang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Stress Response ◽  
Transcriptional Activation ◽  
Dominant Role ◽  
State Level ◽  
Energy Conversion Efficiency ◽  
Nac Transcription Factor ◽  
Wild Plants ◽  
Transgenic Lines

NAC (NAM, ATAF1/2, and CUC2) transcription factors are ubiquitously distributed in eukaryotes and play significant roles in stress response. However, the functional verifications of NACs in Picea (P.) wilsonii remain largely uncharacterized. Here, we identified the NAC transcription factor PwNAC11 as a mediator of drought stress, which was significantly upregulated in P. wilsonii under drought and abscisic acid (ABA) treatments. Yeast two-hybrid assays showed that both the full length and C-terminal of PwNAC11 had transcriptional activation activity and PwNAC11 protein cannot form a homodimer by itself. Subcellular observation demonstrated that PwNAC11 protein was located in nucleus. The overexpression of PwNAC11 in Arabidopsis obviously improved the tolerance to drought stress but delayed flowering time under nonstress conditions. The steady-state level of antioxidant enzymes’ activities and light energy conversion efficiency were significantly increased in PwNAC11 transgenic lines under dehydration compared to wild plants. PwNAC11 transgenic lines showed hypersensitivity to ABA and PwNAC11 activated the expression of the downstream gene ERD1 by binding to ABA-responsive elements (ABREs) instead of drought-responsive elements (DREs). Genetic evidence demonstrated that PwNAC11 physically interacted with an ABA-induced protein—ABRE Binding Factor3 (ABF3)—and promoted the activation of ERD1 promoter, which implied an ABA-dependent signaling cascade controlled by PwNAC11. In addition, qRT-PCR and yeast assays showed that an ABA-independent gene—DREB2A—was also probably involved in PwNAC11-mediated drought stress response. Taken together, our results provide the evidence that PwNAC11 plays a dominant role in plants positively responding to early drought stress and ABF3 and DREB2A synergistically regulate the expression of ERD1.

scholarly journals The Legume miR1514a modulates a NAC transcription factor transcript to trigger phasiRNA formation in response to drought

2016 ◽  
pp. erw380 ◽  
Author(s):  
Guadalupe Sosa-Valencia ◽  
Miguel Palomar ◽  
Alejandra A. Covarrubias ◽  
José L. Reyes
Keyword(s):  
Transcription Factor ◽  
Nac Transcription Factor

scholarly journals Global Expressions Landscape of NAC Transcription Factor Family and Their Responses to Abiotic Stresses in Citrullus lanatus

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Xiaolong Lv ◽  
Shanrong Lan ◽  
Kateta Malangisha Guy ◽  
Jinghua Yang ◽  
Mingfang Zhang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abiotic Stresses ◽  
Citrullus Lanatus ◽  
Nac Transcription Factor ◽  
Transcription Factor Family
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