scholarly journals Heterotrimeric G protein mediates ethylene-induced stomatal closure via hydrogen peroxide synthesis in Arabidopsis

2015 ◽  
Vol 82 (1) ◽  
pp. 138-150 ◽  
Author(s):  
Xiao-Min Ge ◽  
Hong-Li Cai ◽  
Xue Lei ◽  
Xue Zhou ◽  
Ming Yue ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
G Protein ◽  
Stomatal Closure ◽  
Heterotrimeric G Protein ◽  
Hydrogen Peroxide Synthesis

scholarly journals Ethylene-induced stomatal closure in Arabidopsis occurs via AtrbohF-mediated hydrogen peroxide synthesis

2006 ◽  
Vol 47 (6) ◽  
pp. 907-916 ◽  
Author(s):  
Radhika Desikan ◽  
Kathryn Last ◽  
Rhian Harrett-Williams ◽  
Cecilia Tagliavia ◽  
Klaus Harter ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Stomatal Closure ◽  
Hydrogen Peroxide Synthesis

The regulator of G-protein signalling protein mediates D-glucose-induced stomatal closure via triggering hydrogen peroxide and nitric oxide production in Arabidopsis

2018 ◽  
Vol 45 (5) ◽  
pp. 509 ◽  
Author(s):  
Shumei Hei ◽  
Zhifeng Liu ◽  
Aixia Huang ◽  
Xiaoping She
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
G Protein ◽  
Stomatal Closure ◽  
H2o2 Production ◽  
No Production ◽  
Α Subunit ◽  
G Protein Signalling ◽  
Guanine Nucleotide Binding ◽  
Nucleotide Binding Proteins

2-Deoxy-D-glucose, 3-O-methyl-D-glucose and D-mannose are all non-metabolisable D-glucose analogues. Among these, 2-deoxy-D-glucose and D-mannose are substrates for hexokinase (HXK). D-sorbitol and D-mannitol are reduced forms of D-glucose and are typically used as comparable osmotic solutes. Similar to 2-deoxy-D-glucose and D-mannose, D-glucose induced stomatal closure in Arabidopsis, whereas 3-O-methyl-D-glucose, D-sorbitol and D-mannitol did not. The data show that the effect of D-glucose on stomata is metabolism-independent, HXK-dependent and irrelevant to osmotic stress. Additionally, the D-glucose induced closure of stomata in wild-type Arabidopsis, but did not in rgs1-1 and rgs1-2 or gpa1-3 and gpa1-4 mutants, indicating that the regulator of G-protein signalling protein (RGS1) and heterotrimeric guanine nucleotide-binding proteins (G proteins)-α subunit (Gα) also mediate the stomatal closure triggered by D-glucose. Furthermore, the effects of D-glucose on hydrogen peroxide (H2O2) or nitric oxide (NO) production and stomatal closure were more significant in AtrbohD or Nia2-1 mutants than in AtrbohF and AtrbohD/F or Nia1-2 and Nia2-5/Nia1-2. The data indicate that H2O2 sourced from AtrbohF and NO generated by Nia1 are essential for D-glucose-mediated stomatal closure. D-glucose-induced H2O2 and NO production in guard cells were completely abolished in rgs1-1 and rgs1-2, which suggests that RGS1 stimulates H2O2 and NO production in D-glucose-induced stomatal closure. Collectively, our data reveal that both HXK and RGS1 are required for D-glucose-mediated stomatal closure. In this context, D-glucose can be sensed by its receptor RGS1, thereby inducing AtrbohF-dependent H2O2 production and Nia1-catalysed NO accumulation, which in turn stimulates stomatal closure.

Effect of G Protein on Stomatal Movement

2012 ◽  
Vol 550-553 ◽  
pp. 1196-1199
Author(s):  
Yuan Hua Zhang
Keyword(s):  
Ascorbic Acid ◽  
G Protein ◽  
Vicia Faba ◽  
Stomatal Closure ◽  
Stomatal Movement ◽  
Signal Molecule ◽  
Heterotrimeric G Protein ◽  
Α Subunit ◽  
Vicia Faba L

By means of pharmacological approach and stomatal analysis，the role of G protein in light-regulated stomatal movement in Vicia faba L. was studied. The result shows that CTX, the activator of heterotrimeric G protein α-subunit, induced stomatal closure under light, but this effect can be reversed by ascorbic acid(Vc) and catalase(CAT). It indicated that G protein was blunted by light, and H2O2 may be one downsteam signal molecule in G protein participated light-regulated stomatal movement.

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