Maize germinal cell initials accommodate hypoxia and precociously express meiotic genes

Timothy Kelliher; Virginia Walbot

doi:10.1111/tpj.12414

A QUANTITATIVE STUDY OF SPERMATOGENESIS IN RATS BEARING HYPOTHALAMIC LESIONS OF MAMMILLARY AND SUPRA-CHIASMATIC NUCLEI

Acta Endocrinologica ◽

10.1530/acta.0.0600276 ◽

1969 ◽

Vol 60 (2) ◽

pp. 276-280

Author(s):

Bernard A. Rüedi

Keyword(s):

Quantitative Analysis ◽

Quantitative Study ◽

Germinal Cell ◽

Suprachiasmatic Nuclei ◽

Hypothalamic Lesions ◽

Cell Counts ◽

Significant Change ◽

Bilateral Lesions ◽

Made In

ABSTRACT A quantitative analysis of spermatogenesis has been made in rats bearing bilateral lesions of the lateral mammillary nuclei or of the suprachiasmatic nuclei of the hypothalamus. There was no significant change in the germinal cell counts in lesioned rats as compared either with normal or with sham operated rats.

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Histologic observations in cryptorchidism: The congenital germinal-cell deficiency of the undescended testis

Journal of Pediatric Surgery ◽

10.1016/0022-3468(69)90487-4 ◽

1969 ◽

Vol 4 (6) ◽

pp. 606-613 ◽

Cited By ~ 47

Author(s):

Graham H. Farrington

Keyword(s):

Undescended Testis ◽

Germinal Cell

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Changes in mRNA length accompany translational regulation of the somatic and testis-specific cytochrome c genes during spermatogenesis in the mouse

Development ◽

10.1242/dev.110.1.249 ◽

1990 ◽

Vol 110 (1) ◽

pp. 249-257

Author(s):

L.E. Hake ◽

A.A. Alcivar ◽

N.B. Hecht

Keyword(s):

Cytochrome C ◽

Translational Regulation ◽

Northern Blot Analysis ◽

Size Class ◽

Mrna Levels ◽

Germinal Cell ◽

Somatic Tissues ◽

Mrna Length ◽

Pachytene Spermatocytes ◽

Germinal Cells

The mouse testis contains two isotypes of cytochrome c, which differ in 14 of 104 amino acids: cytochrome cs is present in all somatic tissues and cytochrome cT is testis specific. The regulation of cytochrome cS and cytochrome cT gene expression during spermatogenesis was examined by Northern blot analysis using specific cDNA probes. Total RNA was isolated from adult tissues, enriched germinal cell populations and polysomal gradients of total testis and isolated germinal cells. Three cytochrome cS mRNAs were detected averaging 1.3 kb, 1.1 kb and 0.7 kb in all tissues examined; an additional 1.7 kb mRNA was observed in testis. Isolated germinal cells through prepuberal pachytene spermatocytes contained only the three smaller mRNAs; the 1.7 kb mRNA was enriched in round spermatids. All three smaller cytochrome cS mRNAs were present on polysomes; the 1.7 kb mRNA was non-polysomal. Cytochrome cT mRNA of 0.6-0.9 kb was detected in testis; mRNA levels were low in early spermatogonia and peaked in prepuberal pachytene spermatocytes. In adult pachytene spermatocytes, a subset of the cytochrome cT mRNAs, 0.7-0.9 kb, was present on polysomes; a shortened size class, 0.6-0.75 kb, was non-polysomal. A distinct, primarily non-polysomal, cytochrome cT 0.7 kb mRNA was present in round spermatids. These results indicate that (1) both cytochrome cS and cytochrome cT mRNAs are present in early meiotic cells, (2) a 1.7 kb cytochrome cS mRNA is post-meiotically expressed and non-polysomal and (3) cytochrome cS and cytochrome cT mRNAs are each developmentally and translationally regulated during spermatogenesis.

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