scholarly journals Precise marker excision system using an animal‐derived piggyBac transposon in plants

2013 ◽  
Vol 77 (3) ◽  
pp. 454-463 ◽  
Author(s):  
Ayako Nishizawa‐Yokoi ◽  
Masaki Endo ◽  
Keishi Osakabe ◽  
Hiroaki Saika ◽  
Seiichi Toki
Keyword(s):  
Piggybac Transposon ◽  
Marker Excision

piggyBac Transposon as a Tool in Genetic Engineering

2016 ◽  
Vol 32 (6) ◽  
pp. 35-44
Author(s):  
I. A. LAPTEV ◽  
N. M. RAEVSKAYA ◽  
N. A. FILIMONOVA ◽  
S. P. SINEOKY
Keyword(s):  
Genetic Engineering ◽  
Piggybac Transposon

scholarly journals Generation of tetracycline-controllable CYP3A4-expressing Caco-2 cells by the piggyBac transposon system

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Moe Ichikawa ◽  
Hiroki Akamine ◽  
Michika Murata ◽  
Sumito Ito ◽  
Kazuo Takayama ◽  
...  
Keyword(s):  
Small Intestine ◽  
Drug Absorption ◽  
Cell Model ◽  
Negative Effects ◽  
Piggybac Transposon ◽  
Drug Metabolizing Enzyme ◽  
Metabolizing Enzyme ◽  
Cyp3a4 Expression

AbstractCaco-2 cells are widely used as an in vitro intestinal epithelial cell model because they can form a monolayer and predict drug absorption with high accuracy. However, Caco-2 cells hardly express cytochrome P450 (CYP), a drug-metabolizing enzyme. It is known that CYP3A4 is the dominant drug-metabolizing enzyme in human small intestine. In this study, we generated CYP3A4-expressing Caco-2 (CYP3A4-Caco-2) cells and attempted to establish a model that can simultaneously evaluate drug absorption and metabolism. CYP3A4-Caco-2 cells were generated by piggyBac transposon vectors. A tetracycline-controllable CYP3A4 expression cassette (tet-on system) was stably transduced into Caco-2 cells, thus regulating the levels of CYP3A4 expression depending on the doxycycline concentration. The CYP3A4 expression levels in CYP3A4-Caco-2 cells cultured in the presence of doxycycline were similar to or higher than those of adult small intestine. The CYP3A4-Caco-2 cells had enough ability to metabolize midazolam, a substrate of CYP3A4. CYP3A4 overexpression had no negative effects on cell proliferation, barrier function, and P-glycoprotein activity in Caco-2 cells. Thus, we succeeded in establishing Caco-2 cells with CYP3A4 metabolizing activity comparable to in vivo human intestinal tissue. This cell line would be useful in pharmaceutical studies as a model that can simultaneously evaluate drug absorption and metabolism.

scholarly journals A piggyBac Transposon Gene Trap for the Analysis of Gene Expression and Function in Drosophila

Genetics ◽  
2004 ◽  
Vol 167 (4) ◽  
pp. 1801-1811 ◽  
Author(s):  
Christopher P. Bonin ◽  
Richard S. Mann
Keyword(s):  
Gene Expression ◽  
Gene Trap ◽  
Piggybac Transposon ◽  
And Function

The piggyBac transposon is an integrating non-viral gene transfer vector that enhances the efficiency of GDEPT

2009 ◽  
Vol 33 (4) ◽  
pp. 509-515 ◽  
Author(s):  
Yu Kang ◽  
Xiao-Yan Zhang ◽  
Wei Jiang ◽  
Chao-Qun Wu ◽  
Chun-Mei Chen ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Viral Gene ◽  
Piggybac Transposon ◽  
Transfer Vector ◽  
Gene Transfer Vector ◽  
Viral Gene Transfer

scholarly journals Improved gene targeting in C. elegans using counter-selection and Flp-mediated marker excision

Genomics ◽  
2010 ◽  
Vol 95 (1) ◽  
pp. 37-46 ◽  
Author(s):  
Rafael P. Vázquez-Manrique ◽  
James C. Legg ◽  
Birgitta Olofsson ◽  
Sung Ly ◽  
Howard A. Baylis
Keyword(s):  
Gene Targeting ◽  
C Elegans ◽  
Counter Selection ◽  
Marker Excision

Molecular evolutionary analysis of the widespread piggyBac transposon family and related "domesticated" sequences

2003 ◽  
Vol 270 (2) ◽  
pp. 173-180 ◽  
Author(s):  
A. Sarkar ◽  
C. Sim ◽  
Y. S. Hong ◽  
J. R. Hogan ◽  
M. J. Fraser ◽  
...  
Keyword(s):  
Evolutionary Analysis ◽  
Piggybac Transposon ◽  
Molecular Evolutionary Analysis

scholarly journals Bioengineering hemophilia A–specific microvascular grafts for delivery of full-length factor VIII into the bloodstream

2019 ◽  
Vol 3 (24) ◽  
pp. 4166-4176 ◽  
Author(s):  
Joseph Neumeyer ◽  
Ruei-Zeng Lin ◽  
Kai Wang ◽  
Xuechong Hong ◽  
Tien Hua ◽  
...  
Keyword(s):  
Genetic Engineering ◽  
Factor Viii ◽  
Hemophilia A ◽  
Scid Mice ◽  
Full Length ◽  
Piggybac Transposon ◽  
Key Points

Key Points HA-specific iPSC-derived ECs overexpress full-length F8 after genetic engineering via a piggyBac transposon system. Bioengineered microvascular grafts deliver full-length FVIII into the bloodstream and restore hemostasis in hemophilic SCID mice.

Sign in / Sign up

Export Citation Format

Share Document