The role of tubal patency tests and tubal surgery in the era of assisted reproductive techniques

2014 ◽  
Vol 16 (1) ◽  
pp. 37-45 ◽  
Author(s):  
Yalanadu N Suresh ◽  
Nitish N Narvekar
Keyword(s):  
Assisted Reproductive Techniques ◽  
Tubal Patency ◽  
Reproductive Techniques ◽  
Tubal Surgery

scholarly journals Role of hysteroscopy prior to assisted reproductive techniques

2009 ◽  
Vol 1 (1) ◽  
pp. 27 ◽  
Author(s):  
Nandita Palshetkar ◽  
Hrishikesh Pai ◽  
Suchita Pisat
Keyword(s):  
Assisted Reproductive Techniques ◽  
Reproductive Techniques

scholarly journals The role of epigenetic factors in descendants from Assisted Reproductive Techniques (ART)

2021 ◽  
Vol 20 (1) ◽  
pp. 25-30
Author(s):  
Vasilios Pergialiotis ◽  
Anastasia Prodromidou ◽  
Evangelia Dimitroulia ◽  
Dimitrios Loutradis
Keyword(s):  
Potential Effect ◽  
Mechanisms Of Action ◽  
Assisted Reproductive Techniques ◽  
Epigenetic Changes ◽  
Epigenetic Factors ◽  
Vitro Fertilization ◽  
Reproductive Techniques ◽  
Art Research

Altogether, Assisted Reproductive Techniques (ART) are likely to cause some epigenetic changes in the offspring, which might consist the molecular basis of complex characteristics and diseases. The present review contributes a large biochemical dataset of a well-defined group of pre- pubertal in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) conceived children in order to detect the potential effect in the offspring’s health. Additionally, the relevant usefulness of metabolomics and proteomics are also investigated. The outcomes indicate early insulin resistance in ICSI-offspring which can set the basis for further research in the field so as to identify the respective pathophysiological pathways and mechanisms of action. The data support that metabolomics may unravel metabolic variances before they become clinically or biochemically evident, underlining its utility in the ART research.

The impact of endometriosis on the outcome of assisted reproductive techniques: role of fertility preservation

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Daniel Necula ◽  
Andrea Gmuer ◽  
Jérôme Mathis
Keyword(s):  
Fertility Preservation ◽  
Ovarian Tissue ◽  
Assisted Reproductive Techniques ◽  
Ovarian Tissue Cryopreservation ◽  
Individual Treatment ◽  
Multidisciplinary Teams ◽  
Reproductive Techniques ◽  
The Impact

Abstract Endometriosis is one of the most common diseases associated with infertility. It requires different therapeutic approaches, depending on the type, whether superficial, ovarian or deep endometriotic nodules, clinical presentation, age or origin of infertility. Patients with endometriosis should be considered a group of high-risk for infertility and any intervention should be planned taking this consideration into account. For many years, surgery was the only tool in improving fertility outcomes. Nowadays, it remains the gold standard for the treatment of endometriosis, but it should be carefully used because of its harmful potential, leading to premature ovarian insufficiency and further infertility. With the development of modern assisted reproductive techniques (ART), which can offer better results in terms of fertility, the role of surgery progressively decreased. Nowadays, therapeutic approach is complex and multidisciplinary teams should be guiding individual treatment for each woman. Options include medical treatment, surgery, in vitro fertilization (IVF) or oocytes/embryo/ovarian tissue cryopreservation. There is a constant effort in developing diagnostic scores, in order to predict risk of infertility and create standard of practice, offering a long-term approach in terms of fertility preservation and quality of life.

O-217 Bitter Taste Receptors expression in human follicular cells: new perspectives in female fertility

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
A Luddi ◽  
B Semplici ◽  
F P Luongo ◽  
L Governini ◽  
R Ponchia ◽  
...  
Keyword(s):  
Western Blot ◽  
Reproductive System ◽  
Female Reproductive Tract ◽  
Assisted Reproductive Techniques ◽  
Taste Receptors ◽  
Female Reproductive System ◽  
Female Reproduction ◽  
Follicular Cells ◽  
Reproductive Techniques

Abstract Study question Bitter TasteReceptors (TAS2Rs)role in female reproductive system cells: potential implications in mechanisms underlying oocyte maturation and sperm-oocytes interaction. Summary answer TAS2Rs and genes involved in their transduction cascade are differentially expressed in granulosa (GCs)and cumulus cells(CCs). What is known already TASRs expression can be found also in extraoral location wherein their function appears less obvious. TASRs are reported to be involved in signal transduction cascade induced by chemotactic activation in spermatozoa and the expression of TAS2Rs in ejaculated human sperm has been demonstrated. The presence of these receptors in male reproductive system and in sperm gives cues to investigate their possible role in sperm-oocyte interaction. Functional implications have been collected indicating that taste receptors are also important to increase the number of highly fertilization-competent sperm cells within the female genital tract hypothesizing a role in the field of female reproduction. Study design, size, duration We enrolled for this study 30patients undergoing IVF cycles because of couple infertilityfrom June 2019 to October 2020at the UOSA of Assisted Reproductive techniques, Siena University Hospital(Italy). Participants/materials, setting, methods Female patients referring to UOSA of Assisted Reproductive techniques(median age 35 years) underwent a personalised controlled ovarian hyperstimulation protocol. After oocyte pickup, GCs were isolated from the follicular fluid through differential gradient. CCs were collected after oocytes denuding. TAS2Rs and genes involved in the transduction cascade elicited expression/localization in both GCs and CCs were confirmed by Droplet Digital PCR, western blot andimmunofluorescence. Main results and the role of chance For the first time, the expression and cellular localization of the TAS2Rs (TAS2R3, TAS2R4, TAS2R14, TAS2R19 and TAS2R43), their G-coupled proteins (GNAT1 orα-transducinandGNAT3 or α-gustducin) and enzymes involved in the transduction signal (PDE4A, TRPM5 and PLCB2)were demonstrated in the female reproductive system. Overall expression of TAS2Rs emerged higher in GCs than in CCs, confirming the specific molecular fingerprinting during differentiation of ovarian somatic cells. TAS2R14 is the most expressed gene in both GCs and CCs, this could account for its potential involvement in follicular cells physiology and/or for a key role of this receptor in fertilization, as supported by data showing TAS2R14 to be correlated with sperm progressive motility. We demonstrated a positive correlation in GCs between the expression of the TAS2Rs and GNAT3; interestingly, when each subset of TAS2Rs genes was correlated with the signaling gene, TAS2R14 emerged as the one with the higher correlation with GNAT3. Immunofluorescence showed different localization of TAS2Rs and their G-coupled proteins between GCs and CCs. Interestingly some of them presented some fluorescent granules, suggesting a possible involvement of proteins in membrane trafficking. Finally, results of G-coupled proteins western blot, revealed the higher expression of α-gustducin then α-transducin, confirming the gene expression. Limitations, reasons for caution All findings have to be validated in a larger cohort. Moreover, our data pave the way to the understanding of biological functions exerted by these receptors in the female reproductive tract. Wider implications of the findings Further studies might contribute to better understanding the physiologic role of taste receptorsfemale reproductive system. This should becrucial to clarify the role of these receptors in maturation or competence acquiring of oocytes, or also in sperm-oocytes attraction and recognition, crucial point in fertilization process Trial registration number Not applicable

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