Nucleic acid‐targeted pathogen reduction technique in red blood cells by UV‐generated oxygen radicals for optimising recipient safety

2020 ◽  
Vol 30 (1) ◽  
pp. 51-60
Author(s):  
Qiang Zhang ◽  
Chunxi Wu ◽  
Yahan Fan ◽  
Ting Xu ◽  
Qiang Meng ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Oxygen Radicals ◽  
Reduction Technique ◽  
Pathogen Reduction

scholarly journals Bat Red Blood Cells express Nucleic Acid Sensing Receptors and bind RNA and DNA

2022 ◽  
Author(s):  
LK Metthew Lam ◽  
Jane Dobkin ◽  
Kaitlyn A. Eckart ◽  
Ian Gereg ◽  
Andrew DiSalvo ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Red Blood Cells ◽  
Immune Function ◽  
Blood Cells ◽  
Toll Like Receptors ◽  
Cpg Dna ◽  
Human Rbcs ◽  
Insight Into ◽  
Rna And Dna

Red blood cells (RBCs) demonstrate immunomodulatory capabilities through the expression of nucleic acid sensors. Little is known about bat RBCs, and no studies have examined the immune function of bat erythrocytes. Here we show that bat RBCs express the nucleic acid-sensing Toll-like receptors TLR7 and TLR9 and bind the nucleic acid ligands, single-stranded RNA, and CpG DNA. Collectively, these data suggest that, like human RBCs, bat erythrocytes possess immune function and may be reservoirs for nucleic acids. These findings provide unique insight into bat immunity and may uncover potential mechanisms by which virulent pathogens in humans are concealed in bats.

Riboflavin-ultraviolet light pathogen reduction treatment does not impact the immunogenicity of murine red blood cells

Transfusion ◽  
2015 ◽  
Vol 56 (4) ◽  
pp. 863-872 ◽  
Author(s):  
Christopher A. Tormey ◽  
Manjula Santhanakrishnan ◽  
Nicole H. Smith ◽  
Jingchun Liu ◽  
Susanne Marschner ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Ultraviolet Light ◽  
Blood Cells ◽  
Reduction Treatment ◽  
Pathogen Reduction

Sequence analysis confirmation of transfusion-transmitted hepatitis C by red blood cells that tested negative by minipool hepatitis C virus nucleic acid testing

Transfusion ◽  
2007 ◽  
Vol 47 (6) ◽  
pp. 1102-1104 ◽  
Author(s):  
Piotr Grabarczyk ◽  
Agnieszka Gronowska ◽  
Ewa Brojer ◽  
Magdalena Letowska ◽  
Piotr Radziwon
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Nucleic Acid ◽  
Sequence Analysis ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Nucleic Acid Testing

scholarly journals Studies on the Nucleic Acids of the Malaria Parasite, Plasmodium Berghei (Vincke & Lips)

1953 ◽  
Vol 6 (2) ◽  
pp. 234 ◽  
Author(s):  
PR Whitfeld
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Red Blood Cells ◽  
Plasmodium Berghei ◽  
Acid Content ◽  
Blood Cells ◽  
Malaria Parasite ◽  
Nucleic Acid Content ◽  
Solid Residue ◽  
Parasite Plasmodium

Changes in� the nucleic acid content of the solid residue obtained by haemolysing the blood of mice infected with Plasmodium berghei have been examined. The residue from blood in which 25 per cent. of the red blood cells were parasitized contained 20-25 times as much ribosenucleic acid (RNA) and 12 times as much desoxyribosenucleic acid (DNA) as the residue from uninfected blood.

Impact of Nucleic Acid Testing for Human Immunodeficiency Virus and Hepatitis C Virus on Blood Product Availability, Outdating, and Patient Safety

2002 ◽  
Vol 126 (12) ◽  
pp. 1463-1466
Author(s):  
Laurence A. Sherman
Keyword(s):  
Human Immunodeficiency Virus ◽  
Patient Safety ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Nucleic Acid ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Nucleic Acid Testing ◽  
Immunodeficiency Virus ◽  
The Impact

Abstract Context.—Limited data are available about the impact of nucleic acid testing for human immunodeficiency virus and hepatitis C virus in donated blood as part of a nationwide investigational study that affected greater than 90% of the blood supply. Objective.—To assess the impact of nucleic acid testing on supply, outdating, and patient safety. Design.—Participants in the College of American Pathologists 2001 American Association of Blood Banks/College of American Pathologists Viral Marker C survey were asked questions about supply, outdating, and implementation of a full quarantine of blood pending nucleic acid testing results. The number of respondents for each question ranged from 197 to 219 for blood centers and from 462 to 504 for hospitals. Results.—Shortages were more common for platelets (29% and 23% of blood centers and hospitals, respectively) than for red blood cells (13%, 11%). Similarly, outdating of platelets (13%, 11%) was more common than outdating of red blood cells; outdating of red blood cells was negligible for both blood centers and hospitals. Forty-two percent of blood centers did not meet the mid 2000 target date for quarantining red blood cells, and 18% were not quarantining as of September 2001. The hospital figures were 66% not quarantining in mid 2000 and 39% not quarantining as of September 2001. Higher proportions of centers and hospitals were not quarantining platelets at these 2 dates. Conclusions.—Unfavorable trends in both blood shortages and outdating were attributed to nucleic acid testing. Greater effects may have been masked by delayed implementation of full quarantine nationwide. This delay meant continued patient risk, and lack of full benefit, in a trial that was in effect a national standard. In the future, added systems will be needed for similar new endeavors to ensure uniformity of care and to avoid shortages.

Loading of Human Red Blood Cells with DNA and RNA

1976 ◽  
Vol 31 (3-4) ◽  
pp. 149-151 ◽  
Author(s):  
Dieter Auer ◽  
Gerhard Brandner
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Human Erythrocytes ◽  
Viral Dna ◽  
External Concentration ◽  
Dna And Rna ◽  
Human Red Blood Cells ◽  
Hank’S Solution

Abstract Human erythrocytes were suspended in Hank’s solution containing mammalian or viral DNA or RNA. After dialysis at 0 °C first against water and subsequently against Hank’s solution, and a further incubation at 37 °C , the erythrocytes were found to be loaded with the nucleic acids. The nucleic acid trapped in the erythrocytes exhibited up to 35 per cent of the external concentration.

scholarly journals Red Blood Cells Function as DNA Sensors

2021 ◽  
Author(s):  
Metthew Lam ◽  
Sophia Murphy ◽  
Dimitra Kokkinaki ◽  
Alessandro Venosa ◽  
Scott Sherrill-Mix ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Nucleic Acid ◽  
Immune Responses ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Surface Expression ◽  
Innate Immune ◽  
Nucleic Acid Detection ◽  
Oxygen Carriers ◽  
Cell Clearance

AbstractErythrocytes have long been mistaken as exclusively inert oxygen carriers lacking immune function. Here we show that red blood cells (RBCs) serve as immune sensors through surface expression of the nucleic acid-sensing toll-like receptor 9 (TLR9), a classically endosomal receptor that initiates immune responses following the detection of unmethylated CpG motifs present in pathogen and mitochondrial DNA. Mammalian RBCs express TLR9 on their surface and bind CpG-containing bacterial, malarial, and mitochondrial DNA. Erythrocyte-bound CpG DNA increases during infection, and CpG-carrying RBCs trigger accelerated erythrophagocytosis and innate immune activation characterized by RBC-TLR9 dependent local and systemic cytokine production. Thus, RBC nucleic acid detection and capture regulates red cell clearance and immune responses and provides evidence for RBCs as innate immune sentinels during pathologic states.One Sentence SummaryThe ability of RBCs to detect and bind cell-free nucleic acids contributes to immunity during acute inflammatory states.

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