Sequential measurement of Trypanosoma cruzi parasitic load in endomyocardial biopsies for early detection and follow‐up of Chagas disease reactivation after heart transplantation

2019 ◽  
Vol 22 (1) ◽  
Author(s):  
Luiz Alberto Benvenuti ◽  
Alessandra Roggério ◽  
Anna Shoko Nishiya ◽  
Sandrigo Mangini ◽  
José Eduardo Levi
Keyword(s):  
Early Detection ◽  
Trypanosoma Cruzi ◽  
Heart Transplantation ◽  
Chagas Disease ◽  
Sequential Measurement ◽  
Parasitic Load ◽  
Disease Reactivation

Trypanosoma cruzi persistence in the native heart is associated with high-grade myocarditis, but not with Chagas’ disease reactivation after heart transplantation

2014 ◽  
Vol 33 (7) ◽  
pp. 698-703 ◽  
Author(s):  
Luiz A. Benvenuti ◽  
Alessandra Roggério ◽  
Anna S. Nishiya ◽  
Silvia V. Campos ◽  
Alfredo I. Fiorelli ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Heart Transplantation ◽  
Chagas Disease ◽  
High Grade ◽  
Disease Reactivation

Usefulness of PCR Strategies For Early Diagnosis of Chagas' Disease Reactivation and Treatment Follow-Up in Heart Transplantation

2007 ◽  
Vol 7 (6) ◽  
pp. 1633-1640 ◽  
Author(s):  
M. Diez ◽  
L. Favaloro ◽  
A. Bertolotti ◽  
J. M. Burgos ◽  
C. Vigliano ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Heart Transplantation ◽  
Chagas Disease ◽  
Disease Reactivation

scholarly journals Molecular evaluation of Chagas disease reactivation and treatment follow-up in HIV coinfected patients

2018 ◽  
Vol 73 ◽  
pp. 322
Author(s):  
M. Fernandez ◽  
S. Besuschio ◽  
D. Nicita ◽  
V. Latini ◽  
M.L. Biondi ◽  
...  
Keyword(s):  
Chagas Disease ◽  
Disease Reactivation ◽  
Molecular Evaluation

Probing for Trypanosoma cruzi Cell Surface Glycobiomarkers for the Diagnosis and Follow-Up of Chemotherapy of Chagas Disease

2017 ◽  
pp. 195-211
Author(s):  
Nathaniel S. Schocker ◽  
Susana Portillo ◽  
Roger A. Ashmus ◽  
Carlos R. N. Brito ◽  
Igor E. Silva ◽  
...  
Keyword(s):  
Cell Surface ◽  
Trypanosoma Cruzi ◽  
Chagas Disease

scholarly journals Long term follow-up of Trypanosoma cruzi infection and Chagas disease manifestations in mice treated with benznidazole or posaconazole

2020 ◽  
Vol 14 (9) ◽  
pp. e0008726
Author(s):  
Claudia Magalhães Calvet ◽  
Tatiana Araújo Silva ◽  
Diane Thomas ◽  
Brian Suzuki ◽  
Ken Hirata ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Long Term Follow Up ◽  
Cruzi Infection

scholarly journals The Trypomastigote Small Surface Antigen from Trypanosoma cruzi Improves Treatment Evaluation and Diagnosis in Pediatric Chagas Disease

2017 ◽  
Vol 55 (12) ◽  
pp. 3444-3453 ◽  
Author(s):  
Virginia Balouz ◽  
Luciano J. Melli ◽  
Romina Volcovich ◽  
Guillermo Moscatelli ◽  
Samanta Moroni ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Surface Antigen ◽  
Rapid Assessment ◽  
Drug Efficacy ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Tests ◽  
Small Surface ◽  
Content Type

ABSTRACTChagas disease is caused by the protozoan parasiteTrypanosoma cruzi. Assessment of parasitological cure upon treatment with available drugs relies on achieving consistent negative results in conventional parasitological and serological tests, which may take years to assess. Here, we evaluated the use of a recombinantT. cruziantigen termed trypomastigote small surface antigen (TSSA) as an early serological marker of drug efficacy inT. cruzi-infected children. A cohort of 78 pediatric patients born toT. cruzi-infected mothers was included in this study. Only 39 of the children were infected withT. cruzi, and they were immediately treated with trypanocidal drugs. Serological responses against TSSA were evaluated in infected and noninfected populations during the follow-up period using an in-house enzyme-linked immunosorbent assay (ELISA) and compared to conventional serological methods. Anti-TSSA antibody titers decreased significantly faster than anti-whole parasite antibodies detected by conventional serology both inT. cruzi-infected patients undergoing effective treatment and in those not infected. The differential kinetics allowed a significant reduction in the required follow-up periods to evaluate therapeutic responses or to rule out maternal-fetal transmission. Finally, we present the case of a congenitally infected patient with an atypical course in whom TSSA provided an early marker forT. cruziinfection. In conclusion, we showed that TSSA was efficacious both for rapid assessment of treatment efficiency and for early negative diagnosis in infants at risk of congenitalT. cruziinfection. Based upon these findings we propose the inclusion of TSSA for refining the posttherapeutic cure criterion and other diagnostic needs in pediatric Chagas disease.

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