Isolated Tumor Cells in Sentinel Lymph Nodes of Invasive Breast Cancer: Cell Displacement or Metastasis?

2014 ◽  
Vol 20 (5) ◽  
pp. 502-507 ◽  
Author(s):  
Jean-Christophe Tille ◽  
Pierre Loubeyre ◽  
Alexandre Bodmer ◽  
Anne-Sophie Jannot Berthier ◽  
Alexandre Rozenholc ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Lymph Nodes ◽  
Tumor Cells ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Invasive Breast Cancer ◽  
Sentinel Lymph Nodes ◽  
Isolated Tumor Cells ◽  
Invasive Breast Cancer Cell

Isolated tumor cells in breast cancer sentinel lymph nodes: displacement or metastases? An immunohistochemical study

2009 ◽  
Vol 40 (6) ◽  
pp. 778-782 ◽  
Author(s):  
Carolien H.M. van Deurzen ◽  
Peter C. de Bruin ◽  
Ron Koelemij ◽  
Richard Hillegersberg ◽  
Paul J. van Diest
Keyword(s):  
Breast Cancer ◽  
Lymph Nodes ◽  
Tumor Cells ◽  
Immunohistochemical Study ◽  
Sentinel Lymph Nodes ◽  
Isolated Tumor Cells

scholarly journals Chlortetracycline, a Novel Arf Inhibitor That Decreases the Arf6-Dependent Invasive Properties of Breast Cancer Cells

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 969
Author(s):  
Eric Macia ◽  
Monserrat Vazquez-Rojas ◽  
Alessia Robiolo ◽  
Racha Fayad ◽  
Sophie Abélanet ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Invasive Breast Cancer ◽  
Lipid Membranes ◽  
Molecular Mechanisms ◽  
Biochemical Characterization ◽  
Breast Cancer Cell Lines ◽  
Metastatic Tumour ◽  
Invasive Breast Cancer Cell

Breast cancer is a major disease for women worldwide, where mortality is associated with tumour cell dissemination to distant organs. While the number of efficient anticancer therapies increased in the past 20 years, treatments targeting the invasive properties of metastatic tumour cells are still awaited. Various studies analysing invasive breast cancer cell lines have demonstrated that Arf6 is an important player of the migratory and invasive processes. These observations make Arf6 and its regulators potential therapeutic targets. As of today, no drug effective against Arf6 has been identified, with one explanation being that the activation of Arf6 is dependent on the presence of lipid membranes that are rarely included in drug screening. To overcome this issue we have set up a fluorescence-based high throughput screening that follows overtime the activation of Arf6 at the surface of lipid membranes. Using this unique screening assay, we isolated several compounds that affect Arf6 activation, among which the antibiotic chlortetracycline (CTC) appeared to be the most promising. In this report, we describe CTC in vitro biochemical characterization and show that it blocks both the Arf6-stimulated collective migration and cell invasion in a 3D collagen I gel of the invasive breast cancer cell line MDA-MB-231. Thus, CTC appears as a promising hit to target deadly metastatic dissemination and a powerful tool to unravel the molecular mechanisms of Arf6-mediated invasive processes.

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