Examining bull semen for residues of Schmallenberg virus RNA

2021 ◽  
Author(s):  
Akbar Dastjerdi ◽  
S. Anna La Rocca ◽  
Siva Karuna ◽  
Christopher Finnegan ◽  
Julie Peake ◽  
...  
Keyword(s):  
Schmallenberg Virus ◽  
Bull Semen ◽  
Virus Rna

scholarly journals Detection of Schmallenberg Virus RNA in Bull Semen in Poland

2016 ◽  
Vol 19 (3) ◽  
pp. 655-657 ◽  
Author(s):  
J. Kęsik-Maliszewska ◽  
M. Larska
Keyword(s):  
Extraction Method ◽  
Rna Extraction ◽  
Viral Rna ◽  
Schmallenberg Virus ◽  
Rt Pcr ◽  
Bovine Semen ◽  
Bull Semen ◽  
Virus Rna ◽  
Virus Excretion ◽  
Venereal Transmission

Abstract The detection of Schmallenberg virus (SBV) in the breeding bull semen raised the question of the possibility of venereal transmission of SBV which could result in cost-intensive restrictions in the trade of bovine semen. In order to evaluate the presence of SBV RNA in bovine semen, 131 bull semen samples from four locations in Poland collected between 2013 and 2015 were analysed by RT-PCR for viral RNA. SBV RNA was detected in 5.3% of the samples. The study has revealed that application of an appropriate RNA extraction method is crucial to detect virus excretion via semen.

Organ distribution of Schmallenberg virus RNA in malformed newborns

2012 ◽  
Vol 159 (1-2) ◽  
pp. 236-238 ◽  
Author(s):  
S. Bilk ◽  
C. Schulze ◽  
M. Fischer ◽  
M. Beer ◽  
A. Hlinak ◽  
...  
Keyword(s):  
Schmallenberg Virus ◽  
Organ Distribution ◽  
Virus Rna

scholarly journals Evidence of excretion of Schmallenberg virus in bull semen

2014 ◽  
Vol 45 (1) ◽  
pp. 37 ◽  
Author(s):  
Claire Ponsart ◽  
Nathalie Pozzi ◽  
Emmanuel Bréard ◽  
Virginie Catinot ◽  
Guillaume Viard ◽  
...  
Keyword(s):  
Schmallenberg Virus ◽  
Bull Semen

First detection of Schmallenberg virus RNA in bovine semen, Germany, 2012

2013 ◽  
Vol 167 (3-4) ◽  
pp. 289-295 ◽  
Author(s):  
Bernd Hoffmann ◽  
Claudia Schulz ◽  
Martin Beer
Keyword(s):  
Schmallenberg Virus ◽  
Bovine Semen ◽  
Virus Rna

scholarly journals Post-Epidemic Distribution of Schmallenberg Virus in Culicoides Arbovirus Vectors in Poland

Viruses ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 447 ◽  
Author(s):  
Julia Kęsik-Maliszewska ◽  
Magdalena Larska ◽  
Áine B. Collins ◽  
Jerzy Rola
Keyword(s):  
Infection Rate ◽  
Schmallenberg Virus ◽  
Simultaneous Increase ◽  
Virus Rna ◽  
Host Blood ◽  
First Time ◽  
The Relationship ◽  
Pooled Samples ◽  
Culicoides Vectors ◽  
Minimum Infection Rate

Pooled samples of female and male Culicoides midges (5146 and 332 pools, respectively) that corresponded to a total number of 124,957 specimens were collected between 2013–2017 in the vicinity of cattle barns that were distributed throughout Poland were analyzed for the presence of Schmallenberg virus (SBV) RNA. Sixty-six pools tested positive (1.2%) with mean Ct value of 34.95. The maximum likelihood estimated infection rate (MLE) was calculated at 0.53 per 1000 individuals; however, it peaked in 2016 with MLE of 3.7. Viral RNA was detected in C. obsoletus/scoticus complex, C. punctatus, and C. pulicaris pools. Moreover, viral material was present in nulliparous (virgin) Culicoides females (MLE 0.27) and for the first time reported in males (MLE 0.34), which suggests the possibility of transovarial route of SBV or virus RNA transmission, as both do not fed on host blood. The accuracy of targeted versus random SBV surveillance in Culicoides vectors was compared. The relationship between infection rate (expressed as minimum infection rate; MIR), in addition to MLE, was compared with the density of virus infected midges (DIM). In conclusion, the SBV infection rate in the vector was significantly higher in 2016 as compared to other surveillance years; this is consistent with the simultaneous increase in SBV seroprevalence (seroconversion) in ruminants during the same year.

scholarly journals Quality controlled SARS-CoV-2 duplex procedure to reduce time and scarce molecular biology diagnosis reagents

2020 ◽  
Author(s):  
Anaelle Collin ◽  
Carole Chaboteaux ◽  
Veronique Fontaine ◽  
Philippe Lefevre
Keyword(s):  
Molecular Biology ◽  
Working Conditions ◽  
Internal Control ◽  
Internal Controls ◽  
Schmallenberg Virus ◽  
Routine Diagnosis ◽  
Virus Rna ◽  
One Step

The aim of this study was to provide validated procedures allowing to detect the SARS-CoV-2 and an internal control in a unique one-step duplex RT-qPCR. Two internal controls were tested, targeting either the Schmallenberg virus RNA provided by the NARILIS laboratory (University of Namur) with a HEX-labelled probe or a Diagenode Diagnostics internal control with a Cy5-labelled probe. Our results showed that Ct values of the RT-qPCR duplex assay were even smaller in the optimized working conditions, allowing to use the optimized qPCR conditions in routine diagnosis.

CME-Dermatologie 22/Antworten: Masern – Epidemiologie und Klinik

Praxis ◽  
2020 ◽  
Vol 109 (15) ◽  
pp. 1179-1181
Author(s):  
Luc Hari ◽  
Stephan Lautenschlager
Keyword(s):  
Virus Rna ◽  
Sind Eine

Zusammenfassung. Die Masern sind eine hoch ansteckende, akute und febrile Krankheit, die durch eine Infektion mit dem Masernvirus verursacht wird. Gemäss Schätzungen der Weltgesundheitsorganisation (WHO) kommt es bei einer weltweiten Prävalenz von zehn Millionen Patientinnen und Patienten pro Jahr zu rund 142 000 Todesfällen (Mortalitätsrate 1,4 %). Klinisch zeigt sich ein stadienhafter Verlauf: Drei bis vier Tage nach dem initialen katarrhalischen Prodromalstadium mit Fieber und Krankheitsgefühl bildet sich das charakteristische Exanthem aus. Die Diagnosestellung erfolgt primär klinisch, muss aber laborchemisch durch den Nachweis von spezifischen IgM-Antikörpern im Serum oder Virus-RNA im Rachenabstrich bestätigt werden. Die Therapie ist rein supportiv, eine spezifische antivirale Behandlung existiert nicht. Der Prävention kommt daher ein absolut zentraler Stellenwert zu, da es sich bei den Masern um eine durch Impfstoffe vermeidbare Erkrankung handelt.

SCHMALLENBERG VIRUS DISEASE: MOLECULAR BIOLOGY AND CLINICAL PRESENTATION (review)

2012 ◽  
pp. 24-34
Author(s):  
A.V. Sprygin ◽  
◽  
A.V. Kononov ◽  
Yu.Yu. Babin ◽  
V.A. Mishchenko ◽  
...  
Keyword(s):  
Molecular Biology ◽  
Clinical Presentation ◽  
Virus Disease ◽  
Schmallenberg Virus
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