scholarly journals Chronic wasting disease in Norway – a survey of prion protein gene variation among cervids

2021 ◽  
Author(s):  
Mariella Evelyn Güere ◽  
Jørn Våge ◽  
Helene Tharaldsen ◽  
Kjersti Sternang Kvie ◽  
Bård‐Jørgen Bårdsen ◽  
...  
Keyword(s):  
Prion Protein ◽  
Protein Gene ◽  
Chronic Wasting Disease ◽  
Prion Protein Gene ◽  
Wasting Disease ◽  
Gene Variation

scholarly journals Chronic wasting disease associated with prion protein gene (PRNP) variation in Norwegian wild reindeer (Rangifer tarandus)

Prion ◽  
2019 ◽  
Vol 14 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Mariella E. Güere ◽  
Jørn Våge ◽  
Helene Tharaldsen ◽  
Sylvie L. Benestad ◽  
Turid Vikøren ◽  
...  
Keyword(s):  
Prion Protein ◽  
Rangifer Tarandus ◽  
Protein Gene ◽  
Chronic Wasting Disease ◽  
Prion Protein Gene ◽  
Wasting Disease ◽  
Wild Reindeer

scholarly journals Prion protein gene sequence and chronic wasting disease susceptibility in white-tailed deer (Odocoileus virginianus)

Prion ◽  
2015 ◽  
Vol 9 (6) ◽  
pp. 449-462 ◽  
Author(s):  
Adam L Brandt ◽  
Amy C Kelly ◽  
Michelle L Green ◽  
Paul Shelton ◽  
Jan Novakofski ◽  
...  
Keyword(s):  
Odocoileus Virginianus ◽  
Prion Protein ◽  
Gene Sequence ◽  
Disease Susceptibility ◽  
Protein Gene ◽  
Chronic Wasting Disease ◽  
Prion Protein Gene ◽  
Wasting Disease

scholarly journals Prion Protein Gene (PRNP) Sequences Suggest Differing Vulnerability to Chronic Wasting Disease for Florida Key Deer (Odocoileus virginianus clavium) and Columbian White-Tailed Deer (O. v. leucurus)

2020 ◽  
Vol 111 (6) ◽  
pp. 564-572
Author(s):  
Tolulope I N Perrin-Stowe ◽  
Yasuko Ishida ◽  
Emily E Terrill ◽  
Brian C Hamlin ◽  
Linda Penfold ◽  
...  
Keyword(s):  
Odocoileus Virginianus ◽  
Prion Protein ◽  
Protein Gene ◽  
Chronic Wasting Disease ◽  
Synonymous Substitution ◽  
Spongiform Encephalopathy ◽  
Prion Protein Gene ◽  
Wasting Disease ◽  
Genetic Vulnerability ◽  
Key Deer

Abstract Chronic wasting disease (CWD) is a fatal, highly transmissible spongiform encephalopathy caused by an infectious prion protein. CWD is spreading across North American cervids. Studies of the prion protein gene (PRNP) in white-tailed deer (WTD; Odocoileus virginianus) have identified non-synonymous substitutions associated with reduced CWD frequency. Because CWD is spreading rapidly geographically, it may impact cervids of conservation concern. Here, we examined the genetic vulnerability to CWD of 2 subspecies of WTD: the endangered Florida Key deer (O. v. clavium) and the threatened Columbian WTD (O. v. leucurus). In Key deer (n = 48), we identified 3 haplotypes formed by 5 polymorphisms, of which 2 were non-synonymous. The polymorphism c.574G>A, unique to Key deer (29 of 96 chromosomes), encodes a non-synonymous substitution from valine to isoleucine at codon 192. In 91 of 96 chromosomes, Key deer carried c.286G>A (G96S), previously associated with substantially reduced susceptibility to CWD. Key deer may be less genetically susceptible to CWD than many mainland WTD populations. In Columbian WTD (n = 13), 2 haplotypes separated by one synonymous substitution (c.438C>T) were identified. All of the Columbian WTD carried alleles that in other mainland populations are associated with relatively high susceptibility to CWD. While larger sampling is needed, future management plans should consider that Columbian WTD are likely to be genetically more vulnerable to CWD than many other WTD populations. Finally, we suggest that genetic vulnerability to CWD be assessed by sequencing PRNP across other endangered cervids, both wild and in captive breeding facilities.

scholarly journals Characterization of the prion protein gene in axis deer (Axis axis) and implications for susceptibility to chronic wasting disease

Prion ◽  
2021 ◽  
Vol 15 (1) ◽  
pp. 44-52
Author(s):  
Matthew J. Buchholz ◽  
Emily A. Wright ◽  
Blake A. Grisham ◽  
Robert D. Bradley ◽  
Thomas L. Arsuffi ◽  
...  
Keyword(s):  
Prion Protein ◽  
Protein Gene ◽  
Chronic Wasting Disease ◽  
Prion Protein Gene ◽  
Wasting Disease ◽  
Axis Axis ◽  
Axis Deer

Prion protein gene variation among primates

1997 ◽  
Vol 265 (2) ◽  
pp. 257 ◽  
Author(s):  
Hermann M Schätzl ◽  
Maria Da Costa ◽  
Leslie Taylor ◽  
Fred E Cohen ◽  
Stanley B Prusiner
Keyword(s):  
Prion Protein ◽  
Protein Gene ◽  
Prion Protein Gene ◽  
Gene Variation

scholarly journals Age structuring and spatial heterogeneity in prion protein gene (PRNP) polymorphism in white-tailed deer

2020 ◽  
Author(s):  
Tyler K. Chafin ◽  
Marlis R. Douglas ◽  
Bradley T. Martin ◽  
Zachery D. Zbinden ◽  
Christopher R. Middaugh ◽  
...  
Keyword(s):  
Prion Protein ◽  
Protein Gene ◽  
Population Level ◽  
Synonymous Substitution ◽  
Global Scale ◽  
Prion Protein Gene ◽  
Endemic Region ◽  
Wasting Disease ◽  
Increased Susceptibility ◽  
Do So

ABSTRACTChronic-wasting disease (CWD) is a prion-derived fatal neurodegenerative disease that has affected wild cervid populations on a global scale. Susceptibility has been linked unambiguously to several amino acid variants within the prion protein gene (PRNP). Quantifying their distribution across landscapes can provide critical information for agencies attempting to adaptively manage CWD. Here we attempt to further define management implications of PRNP polymorphism by quantifying the contemporary geographic distribution (i.e., phylogeography) of PRNP variants in hunter-harvested white-tailed deer (WTD; Odocoileus virginianus, N=1433) distributed across Arkansas (USA), including a focal spot for CWD since detection of the disease in February 2016. Of these, PRNP variants associated with the well-characterized 96S non-synonymous substitution showed a significant increase in relative frequency among older CWD-positive cohorts. We interpreted this pattern as reflective of a longer life expectancy for 96S genotypes in a CWD-endemic region, suggesting either decreased probabilities of infection or reduced disease progression. Other variants showing statistical signatures of potential increased susceptibility, however, seemingly do so as an artefact of population structure. We also showed marked heterogeneity across the landscape in the prevalence of ‘reduced susceptibility’ genotypes. This may indicate, in turn, that differences in disease susceptibility among WTD in Arkansas are an innate, population-level characteristic that is detectable through phylogeographic analysis.

Evaluation of proteinase K-resistant prion protein (PrPres) in Korean native black goats carrying a potential scrapie-susceptible haplotype of the prion protein gene (PRNP)

2021 ◽  
Author(s):  
Sae-Young Won ◽  
Yong-Chan Kim ◽  
Byung-Hoon Jeong
Keyword(s):  
Linkage Disequilibrium ◽  
Prion Protein ◽  
Prion Disease ◽  
Protein Gene ◽  
Proteinase K ◽  
Broad Host Range ◽  
Strong Linkage Disequilibrium ◽  
Prion Protein Gene ◽  
Wasting Disease ◽  
Strong Linkage

AbstractPrion disease is a fatal neurodegenerative disease with a broad host range in humans and animals. It is caused by proteinase K-resistant prion protein (PrPres). In previous studies, a heterogeneous infection in Cervidae and Caprinae was reported. Chronic wasting disease (CWD) has been frequently reported as the only prion disease in Korea that occurs in livestock. Thus, there is a possibility of transmission of CWD to Korean native black goats. However, PrPres has not been investigated thus far in Korean native black goats. We found strong linkage disequilibrium between c.126G>A and c.414T>C (r2 = 1) and between c.718C>T and c.126G>A (r2 = 0.638). In addition, the haplotype GTGTAAAC (representing codons 42, 102, 127, 138, 143, 146, 218 and 240) showed the highest frequency with 45.1%. Among 41 Korean native black goats, 20 animals (48.78%) were homozygous for the susceptible haplotypes (histidine at codon 143, asparagine at codon 146 and arginine at codon 154). Interestingly, we did not detect PrPres bands in any of the tested animals, including the 20 animals carrying potential scrapie susceptible haplotypes.

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