Molecular characterization of Giardia duodenalis and evidence for cross‐species transmission in Northern Argentina

2021 ◽  
Author(s):  
Sahana Kuthyar ◽  
Martin M. Kowalewski ◽  
Matthew Seabolt ◽  
Dawn M. Roellig ◽  
Thomas R. Gillespie
Keyword(s):  
Molecular Characterization ◽  
Giardia Duodenalis

scholarly journals Molecular Characterization of Giardia duodenalis in Children and Adults Sampled in Algeria

2020 ◽  
Vol 9 (1) ◽  
pp. 54
Author(s):  
Salem Belkessa ◽  
Daniel Thomas-Lopez ◽  
Karim Houali ◽  
Farida Ghalmi ◽  
Christen Rune Stensvold
Keyword(s):  
Real Time ◽  
Molecular Characterization ◽  
Real Time Pcr ◽  
Intestinal Parasites ◽  
Giardia Duodenalis ◽  
Specific Pcr ◽  
Stool Samples ◽  
Pcr Targeting ◽  
Assemblage B

The molecular epidemiology of giardiasis in Africa remains unclear. A study was carried out across four hospitals in Algeria. A total of 119 fecal samples from 55 children, 37 adults, and 27 individuals of undetermined age, all scored positive for intestinal parasites by microscopy, and were screened by real-time PCR for Giardia. Molecular characterization of Giardia was performed by assemblage-specific PCR and PCR targeting the triose phosphate isomerase gene (tpi). Of the 119 samples, 80 (67%) were Giardia-positive by real-time PCR. For 48 moderately-highly real-time PCR-positive samples, tpi genotyping assigned 22 samples to Assemblage A and 26 to Assemblage B. Contrary to Assemblage A, Assemblage B exhibited substantial genetic diversity and allelic heterozygosity. Assemblage-specific PCR proved to be specific for discriminating Assemblage A or B but not as sensitive as tpi genotyping. We confirmed that real-time PCR is more sensitive than microscopy for detecting Giardia in stool samples and that robust amplification and sequencing of the tpi gene is feasible when moderate-to-strongly real-time PCR-positive samples are used. This study is one of the few performed in Africa providing genotyping data on Giardia infections in humans. Both assemblages A and B were commonly seen and not associated with specific sociodemographic data.

Molecular characterization of Giardia duodenalis in dogs from Brazil

2011 ◽  
Vol 110 (1) ◽  
pp. 325-334 ◽  
Author(s):  
Flávio M. Paz e Silva ◽  
Marina M. Monobe ◽  
Raimundo S. Lopes ◽  
João P. Araujo Jr
Keyword(s):  
Molecular Characterization ◽  
Giardia Duodenalis

Molecular characterization of Giardia duodenalis isolates from police and farm dogs in China

2013 ◽  
Vol 135 (2) ◽  
pp. 223-226 ◽  
Author(s):  
Wei Li ◽  
Chengwu Liu ◽  
Yuqiang Yu ◽  
Jianhua Li ◽  
Pengtao Gong ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Giardia Duodenalis

scholarly journals Erratum to: Molecular characterization of Giardia duodenalis in dogs from Brazil

2011 ◽  
Vol 109 (5) ◽  
Author(s):  
Flávio M. Paz e Silva ◽  
Marina M. Monobe ◽  
Raimundo S. Lopes ◽  
João P. Araujo
Keyword(s):  
Molecular Characterization ◽  
Giardia Duodenalis

Molecular characterization of human isolates of Giardia duodenalis from Ethiopia

Acta Tropica ◽  
2007 ◽  
Vol 102 (2) ◽  
pp. 92-99 ◽  
Author(s):  
Tesfaye Gelanew ◽  
Marco Lalle ◽  
Asrat Hailu ◽  
Edoardo Pozio ◽  
Simone M. Cacciò
Keyword(s):  
Molecular Characterization ◽  
Giardia Duodenalis

scholarly journals Detection and Molecular Characterization of Giardia duodenalis in Children Attending Day Care Centers in Majadahonda, Madrid, Central Spain

Medicine ◽  
2014 ◽  
Vol 93 (15) ◽  
pp. e75 ◽  
Author(s):  
Marta Mateo ◽  
María Mateo ◽  
Ana Montoya ◽  
Begoña Bailo ◽  
José M. Saugar ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Day Care ◽  
Giardia Duodenalis ◽  
Central Spain ◽  
Day Care Centers
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