Incursions of rabbit hemorrhagic disease virus 2 in Canada – clinical, molecular and epidemiological investigation

2021 ◽  
Author(s):  
Aruna Ambagala ◽  
Helen Schwantje ◽  
Sonja Laurendeau ◽  
Heindrich Snyman ◽  
Tomy Joseph ◽  
...  
Keyword(s):  
Disease Virus ◽  
Rabbit Hemorrhagic Disease Virus ◽  
Hemorrhagic Disease ◽  
Epidemiological Investigation ◽  
Rabbit Hemorrhagic Disease

scholarly journals A Review on the Methods Used for the Detection and Diagnosis of Rabbit Hemorrhagic Disease Virus (RHDV)

2021 ◽  
Vol 9 (5) ◽  
pp. 972
Author(s):  
Joana Abrantes ◽  
Ana M. Lopes
Keyword(s):  
Disease Virus ◽  
Diagnostic Tests ◽  
High Throughput Sequencing ◽  
Current Knowledge ◽  
European Rabbit ◽  
Rabbit Hemorrhagic Disease Virus ◽  
Hemorrhagic Disease ◽  
Rabbit Hemorrhagic Disease ◽  
Detection And Diagnosis

Since the early 1980s, the European rabbit (Oryctolagus cuniculus) has been threatened by the rabbit hemorrhagic disease (RHD). The disease is caused by a lagovirus of the family Caliciviridae, the rabbit hemorrhagic disease virus (RHDV). The need for detection, identification and further characterization of RHDV led to the development of several diagnostic tests. Owing to the lack of an appropriate cell culture system for in vitro propagation of the virus, much of the methods involved in these tests contributed to our current knowledge on RHD and RHDV and to the development of vaccines to contain the disease. Here, we provide a comprehensive review of the RHDV diagnostic tests used since the first RHD outbreak and that include molecular, histological and serological techniques, ranging from simpler tests initially used, such as the hemagglutination test, to the more recent and sophisticated high-throughput sequencing, along with an overview of their potential and their limitations.

A new variant of rabbit hemorrhagic disease virus G2-like strain isolated in China

2016 ◽  
Vol 215 ◽  
pp. 20-24 ◽  
Author(s):  
Bo Hu ◽  
Zhiyu Fan ◽  
Fang Wang ◽  
Yanhua Song ◽  
Houjun Wei ◽  
...  
Keyword(s):  
Disease Virus ◽  
Rabbit Hemorrhagic Disease Virus ◽  
Hemorrhagic Disease ◽  
Rabbit Hemorrhagic Disease ◽  
New Variant

scholarly journals Minor structural protein VP2 in rabbit hemorrhagic disease virus downregulates the expression of the viral capsid protein VP60

2009 ◽  
Vol 90 (12) ◽  
pp. 2952-2955 ◽  
Author(s):  
Liu Chen ◽  
Guangqing Liu ◽  
Zheng Ni ◽  
Bin Yu ◽  
Tao Yun ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Disease Virus ◽  
Luciferase Assay ◽  
Rabbit Hemorrhagic Disease Virus ◽  
Hemorrhagic Disease ◽  
Mrna Levels ◽  
Structural Protein ◽  
Rabbit Hemorrhagic Disease ◽  
Minor Structural Protein ◽  
Capsid Protein Vp60

Rabbit hemorrhagic disease virus (RHDV) has two structural proteins: the major capsid protein VP60 and the minor capsid protein VP2. VP2 is speculated to play an important role in the virus life cycle. To investigate the effect of VP2 on VP60 expression, three types of experiment (baculovirus–insect cell system, mammalian–luciferase assay system and in vitro coupled transcription/translation system) were used to express VP60 alone or co-expressed with VP2. Both forms of VP60 were able to form virus-like particles in insect cells. Western blot analysis and dual-luciferase assays demonstrated that the presence of VP2 results in downregulation of the expression of VP60 in vivo. Real-time RT-PCR of mRNA levels showed that downregulation of VP60 occurs at the transcriptional level. The ability of the viral minor structural protein VP2 to regulate capsid protein levels may contribute to effective virus infection.

scholarly journals Protection of Rabbits against Rabbit Hemorrhagic Disease Virus by Immunization with the VP60 Protein Expressed in Plants with a Potyvirus-Based Vector

Virology ◽  
2001 ◽  
Vol 280 (2) ◽  
pp. 283-291 ◽  
Author(s):  
María Rosario Fernández-Fernández ◽  
Mercedes Mouriño ◽  
José Rivera ◽  
Francisco Rodríguez ◽  
Juan Plana-Durán ◽  
...  
Keyword(s):  
Disease Virus ◽  
Rabbit Hemorrhagic Disease Virus ◽  
Hemorrhagic Disease ◽  
Rabbit Hemorrhagic Disease

scholarly journals Ecosystem Effects of Variant Rabbit Hemorrhagic Disease Virus, Iberian Peninsula

2014 ◽  
Vol 20 (12) ◽  
pp. 2166-2168 ◽  
Author(s):  
Miguel Delibes-Mateos ◽  
Catarina Ferreira ◽  
Francisco Carro ◽  
Marco A. Escudero ◽  
Christian Gortázar
Keyword(s):  
Iberian Peninsula ◽  
Disease Virus ◽  
Rabbit Hemorrhagic Disease Virus ◽  
Hemorrhagic Disease ◽  
Rabbit Hemorrhagic Disease ◽  
Ecosystem Effects
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