scholarly journals Direct colorimetric LAMP assay for rapid detection of African swine fever virus: A validation study during an outbreak in Vietnam

2020 ◽  
Author(s):  
Diem Hong Tran ◽  
Hau Thi Tran ◽  
Uyen Phuong Le ◽  
Xuan Dang Vu ◽  
Thi Bich Ngoc Trinh ◽  
...  
Keyword(s):  
Validation Study ◽  
Rapid Detection ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Lamp Assay ◽  
Fever Virus

scholarly journals Field Verification of an African Swine Fever Virus Loop-Mediated Isothermal Amplification (LAMP) Assay during an Outbreak in Timor-Leste

Viruses ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1444
Author(s):  
Peter T. Mee ◽  
Shani Wong ◽  
Kim J. O’Riley ◽  
Felisiano da Conceição ◽  
Joanita Bendita da Costa Jong ◽  
...  
Keyword(s):  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Lamp Assay ◽  
Fever Virus ◽  
Isothermal Amplification ◽  
Clinical Samples ◽  
Loop Mediated Isothermal Amplification ◽  
Field Verification ◽  
Extraction Step ◽  
Timor Leste

Recent outbreaks of African swine fever virus (ASFV) have seen the movement of this virus into multiple new regions with devastating impact. Many of these outbreaks are occurring in remote, or resource-limited areas, that do not have access to molecular laboratories. Loop-mediated isothermal amplification (LAMP) is a rapid point of care test that can overcome a range of inhibitors. We outline further development of a real-time ASFV LAMP, including field verification during an outbreak in Timor-Leste. To increase field applicability, the extraction step was removed and an internal amplification control (IAC) was implemented. Assay performance was assessed in six different sample matrices and verified for a range of clinical samples. A LAMP detection limit of 400 copies/rxn was determined based on synthetic positive control spikes. A colourmetric LAMP assay was also assessed on serum samples. Comparison of the LAMP assay to a quantitative polymerase chain reaction (qPCR) was performed on clinical ASFV samples, using both serum and oral/rectal swabs, with a substantial level of agreement observed. The further verification of the ASFV LAMP assay, removal of extraction step, implementation of an IAC and the assessment of a range of sample matrix, further support the use of this assay for rapid in-field detection of ASFV.

scholarly journals Rapid detection of African swine fever virus using Cas12a-based portable paper diagnostics

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Shuhan Lu ◽  
Fang Li ◽  
Qiubing Chen ◽  
Jing Wu ◽  
Junyi Duan ◽  
...  
Keyword(s):  
Rapid Detection ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Paper Diagnostics

Chimeric DNA/LNA-based biosensor for the rapid detection of African swine fever virus

Talanta ◽  
2018 ◽  
Vol 184 ◽  
pp. 35-41 ◽  
Author(s):  
Massimo Biagetti ◽  
Massimiliano Cuccioloni ◽  
Laura Bonfili ◽  
Valentina Cecarini ◽  
Carla Sebastiani ◽  
...  
Keyword(s):  
Rapid Detection ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus

Genetic markers of the African swine fever virus

2020 ◽  
Vol 23 (04) ◽  
pp. 21-26
Author(s):  
A.K. Sibgatullova ◽  
◽  
M.E. Vlasov ◽  
I.A. Titov ◽  
◽  
...  
Keyword(s):  
Genetic Markers ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus
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