Rapid and highly sensitive portable detection of African swine fever virus

2020 ◽  
Author(s):  
Jade Daigle ◽  
Chukwunonso Onyilagha ◽  
Thang Truong ◽  
Van Phan Le ◽  
Bui Thi To Nga ◽  
...  
Keyword(s):  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Portable Detection ◽  
Highly Sensitive

scholarly journals Development and clinical application of a novel CRISPR-Cas12a based assay for the detection of African swine fever virus

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Xiaoying Wang ◽  
Sheng He ◽  
Na Zhao ◽  
Xiaohong Liu ◽  
Yongchang Cao ◽  
...  
Keyword(s):  
African Swine Fever Virus ◽  
Diagnostic Technique ◽  
African Swine Fever ◽  
Cross Reactivity ◽  
Fever Virus ◽  
Effective Vaccine ◽  
Highly Sensitive ◽  
Resource Poor ◽  
Good Consistency ◽  
Virus Genotypes

Abstract Background As no treatment or effective vaccine for African swine fever virus (ASFV) is currently available, a rapid, highly sensitive diagnostic is urgently needed to curb the spread of ASFV. Results Here we designed a novel CRISPR-Cas12a based assay for ASFV detection. To detect different ASFV genotypes, 19 crRNAs were designed to target the conserved p72 gene in ASFV, and several crRNAs with high activity were identified that could be used as alternatives in the event of new ASFV variants. The results showed that the sensitivity of the CRISPR-Cas12a based assay is about ten times higher than either the commercial quantitative PCR (qPCR) kit or the OIE-recommended qPCR. CRISPR-Cas12a based assay could also detect ASFV specifically without cross-reactivity with other important viruses in pigs and various virus genotypes. We also found that longer incubation times increased the detection limits, which could be applied to improve assay outcomes in the detection of weakly positive samples and new ASFV variants. In addition, both the CRISPR-Cas12a based assay and commercial qPCR showed very good consistency. Conclusions In summary, the CRISPR-Cas12a based assay offers a feasible approach and a new diagnostic technique for the diagnosis of ASFV, particularly in resource-poor settings.

scholarly journals Highly Sensitive PCR Assay for Routine Diagnosis of African Swine Fever Virus in Clinical Samples

2003 ◽  
Vol 41 (9) ◽  
pp. 4431-4434 ◽  
Author(s):  
M. Aguero ◽  
J. Fernandez ◽  
L. Romero ◽  
C. Sanchez Mascaraque ◽  
M. Arias ◽  
...  
Keyword(s):  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Clinical Samples ◽  
Pcr Assay ◽  
Routine Diagnosis ◽  
Highly Sensitive

Genetic markers of the African swine fever virus

2020 ◽  
Vol 23 (04) ◽  
pp. 21-26
Author(s):  
A.K. Sibgatullova ◽  
◽  
M.E. Vlasov ◽  
I.A. Titov ◽  
◽  
...  
Keyword(s):  
Genetic Markers ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus

Multigene families in African swine fever virus: family 110.

1990 ◽  
Vol 64 (5) ◽  
pp. 2064-2072 ◽  
Author(s):  
J M Almendral ◽  
F Almazán ◽  
R Blasco ◽  
E Viñuela
Keyword(s):  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Multigene Families ◽  
Virus Family

GS-441524 inhibits African swine fever virus infection in vitro

2021 ◽  
pp. 105081
Author(s):  
Zhao Huang ◽  
Lang Gong ◽  
Zezhong Zheng ◽  
Qi Gao ◽  
Xiongnan Chen ◽  
...  
Keyword(s):  
Virus Infection ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus
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