Genetic diversity of avian hepatitis E virus in China, 2018–2019

2020 ◽  
Vol 67 (6) ◽  
pp. 2403-2407 ◽  
Author(s):  
Qi Su ◽  
Yaqi Liu ◽  
Zhizhong Cui ◽  
Shuang Chang ◽  
Peng Zhao
Keyword(s):  
Genetic Diversity ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Avian Hepatitis E Virus

Infection of Pigs with Avian Hepatitis E Virus (HEV)

2005 ◽  
Author(s):  
C. Kasorndorkbua ◽  
P. J. Thomas ◽  
Patrick G. Halbur ◽  
D. K. Guenette ◽  
F. F. Huang ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Avian Hepatitis E Virus

scholarly journals Identification and genetic diversity of hepatitis E virus in domestic swine from Slovakia

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Anna Jackova ◽  
Katarina Dudasova ◽  
Slavomira Salamunova ◽  
Rene Mandelik ◽  
Jaroslav Novotny ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Developed Countries ◽  
European Countries ◽  
Major Clade ◽  
Information Gap ◽  
Production Stages ◽  
Suckling Piglets ◽  
Domestic Swine

Abstract Background Hepatitis E virus (HEV) is agent causing hepatitis worldwide. Originally considered to be limited to developing countries, this virus was also detected in developed countries. In recent years an increasing number of reports indicate that farmed domestic pigs are widely infected with HEV in several European countries. The HEV status in Slovakia is still missing. Results In this study, the circulation of HEV among domestic swine in Slovakia and genetic diversity of the virus was studied. Overall HEV RNA was detected in 53/388 (13.7, 95% CI: 10.40–17.48%) pig rectal swabs in five production stages (age categories) with statistically significant differences among all the stages. The highest HEV prevalence was observed in weaners 24/81 (29.6, 95% CI: 19.99–40.81%) and then significantly declined in growers and fatteners. No HEV was detected in suckling piglets and sows. Twenty-eight partial sequences of ORF1 (242 bp) and seventeen of ORF2 (304 bp) were analysed. Phylogenetic analysis and p-distance comparisons confirmed in both ORFs that all Slovak HEV sequences belong to the genotype HEV-3, major clade 3abchij with higher identity to 3a and 3i subtypes. Three sequences were outside of all lastly updated HEV-3 subtypes. Conclusion This is the first report to fill the information gap about HEV infection in pigs in Slovakia. The results suggested a lower prevalence of HEV in Slovak pig farms than observed in other European countries. While most HEV isolates were typed as HEV-3 clade 3abchij, three sequences were unclassified.

Identification and characterization of avian hepatitis E virus genotype 2 from chickens with hepatitis-splenomegaly syndrome in Korea

Virus Genes ◽  
2016 ◽  
Vol 52 (5) ◽  
pp. 738-742 ◽  
Author(s):  
Hyun-Woo Moon ◽  
Byung-Woo Lee ◽  
Haan Woo Sung ◽  
Byung-Il Yoon ◽  
Hyuk Moo Kwon
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Virus Genotype ◽  
Genotype 2 ◽  
Avian Hepatitis E Virus ◽  
Identification And Characterization

scholarly journals Identification of two neutralization epitopes on the capsid protein of avian hepatitis E virus

2008 ◽  
Vol 89 (2) ◽  
pp. 500-508 ◽  
Author(s):  
E.-M. Zhou ◽  
H. Guo ◽  
F. F. Huang ◽  
Z. F. Sun ◽  
X. J. Meng
Keyword(s):  
Capsid Protein ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Avian Hepatitis E Virus

scholarly journals Chicken Organic Anion-Transporting Polypeptide 1A2, a Novel Avian Hepatitis E Virus (HEV) ORF2-Interacting Protein, Is Involved in Avian HEV Infection

2019 ◽  
Vol 93 (11) ◽  
Author(s):  
Huixia Li ◽  
Mengnan Fan ◽  
Baoyuan Liu ◽  
Pinpin Ji ◽  
Yiyang Chen ◽  
...  
Keyword(s):  
Viral Infection ◽  
Capsid Protein ◽  
Hepatitis E Virus ◽  
Organic Anion ◽  
Hepatitis E ◽  
Host Cells ◽  
Cell Culture System ◽  
Organic Anion Transporting Polypeptide ◽  
Avian Hepatitis E Virus ◽  
Avian Hev

ABSTRACT Avian hepatitis E virus (HEV) is the main causative agent of big liver and spleen disease in chickens. Due to the absence of a highly effective cell culture system, there are few reports about the interaction between avian HEV and host cells. In this study, organic anion-transporting polypeptide 1A2 (OATP1A2) from chicken liver cells was identified to interact with ap237, a truncated avian HEV capsid protein spanning amino acids 313 to 549, by a glutathione S-transferase (GST) pulldown assay. GST pulldown and indirect enzyme-linked immunosorbent assays (ELISAs) further confirmed that the extracellular domain of OATP1A2 directly binds with ap237. The expression levels of OATP1A2 in host cells are positively correlated with the amounts of ap237 attachment and virus infection. The distribution of OATP1A2 in different tissues is consistent with avian HEV infection in vivo. Finally, when the functions of OATP1A2 in cells are inhibited by its substrates or an inhibitor or blocked by ap237 or anti-OATP1A2 sera, attachment to and infection of host cells by avian HEV are significantly reduced. Collectively, these results displayed for the first time that OATP1A2 interacts with the avian HEV capsid protein and can influence viral infection in host cells. The present study provides new insight to understand the process of avian HEV infection of host cells. IMPORTANCE The process of viral infection is centered around the interaction between the virus and host cells. Due to the lack of a highly effective cell culture system in vitro, there is little understanding about the interaction between avian HEV and its host cells. In this study, a total of seven host proteins were screened in chicken liver cells by a truncated avian HEV capsid protein (ap237) in which the host protein OATP1A2 interacted with ap237. Overexpression of OATP1A2 in the cells can promote ap237 adsorption as well as avian HEV adsorption and infection of the cells. When the function of OATP1A2 in cells was inhibited by substrates or inhibitors, attachment and infection by avian HEV significantly decreased. The distribution of OATP1A2 in different chicken tissues corresponded with that in tissues during avian HEV infection. This is the first finding that OATP1A2 is involved in viral infection of host cells.

Quantification and genetic diversity of Hepatitis E virus in wild boar (Sus scrofa) hunted for domestic consumption in Central Italy

2019 ◽  
Vol 82 ◽  
pp. 194-201 ◽  
Author(s):  
Simona Di Pasquale ◽  
Paola De Santis ◽  
Giuseppina La Rosa ◽  
Kevin Di Domenico ◽  
Marcello Iaconelli ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Wild Boar ◽  
Sus Scrofa ◽  
Hepatitis E Virus ◽  
Central Italy ◽  
Hepatitis E ◽  
Domestic Consumption

Evaluation of recombinant Chinese avian hepatitis E virus (CaHEV) ORF2 and ORF3 proteins for protection of chickens against CaHEV infection

Vaccine ◽  
2017 ◽  
Vol 35 (27) ◽  
pp. 3482-3489 ◽  
Author(s):  
Shahid Faraz Syed ◽  
Yani Sun ◽  
Taofeng Du ◽  
Yiyang Chen ◽  
Baoyuan Liu ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Avian Hepatitis E Virus

The serological prevalence and genetic diversity of hepatitis E virus in farmed rabbits in China

2011 ◽  
Vol 11 (2) ◽  
pp. 476-482 ◽  
Author(s):  
Yansheng Geng ◽  
Chenyan Zhao ◽  
Aijing Song ◽  
Jinheng Wang ◽  
Xiao Zhang ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Hepatitis E Virus ◽  
Hepatitis E

scholarly journals Development and evaluation of a SYBR Green real-time RT-PCR assay for detection of avian hepatitis E virus

2015 ◽  
Vol 11 (1) ◽  
Author(s):  
Qin Zhao ◽  
Sha Xie ◽  
Yani Sun ◽  
Yiyang Chen ◽  
Jiming Gao ◽  
...  
Keyword(s):  
Real Time ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Sybr Green ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Avian Hepatitis E Virus
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