Foot‐and‐mouth disease outbreaks due to an exotic virus serotype A lineage (A/AFRICA/G‐IV) in Algeria in 2017

2018 ◽  
Vol 66 (1) ◽  
pp. 7-13 ◽  
Author(s):  
Giulia Pezzoni ◽  
Arianna Bregoli ◽  
Santina Grazioli ◽  
Ilaria Barbieri ◽  
Hafsa Madani ◽  
...  
Keyword(s):  
Disease Outbreaks ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Serotype A ◽  
Virus Serotype ◽  
Foot And Mouth

scholarly journals Use of ENABL® adjuvant to increase the potency of an adenovirus-vectored foot-and-mouth disease virus serotype A subunit vaccine

Vaccine ◽  
2018 ◽  
Vol 36 (8) ◽  
pp. 1078-1084 ◽  
Author(s):  
José Barrera ◽  
Christopher Schutta ◽  
Melia Pisano ◽  
Marvin J. Grubman ◽  
David A. Brake ◽  
...  
Keyword(s):  
Disease Virus ◽  
Subunit Vaccine ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Serotype A ◽  
Mouth Disease Virus ◽  
Virus Serotype ◽  
Foot And Mouth ◽  
A Subunit

scholarly journals Molecular characterization of foot-and-mouth disease viruses collected from Northern and Central Ethiopia during the 2018 outbreak

2020 ◽  
Vol 13 (3) ◽  
pp. 542-548
Author(s):  
Yeneneh Tesfaye ◽  
Fazlurrahman Khan ◽  
Esayas Gelaye
Keyword(s):  
Gene Sequencing ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Clinical Samples ◽  
Rt Pcr ◽  
Serotype A ◽  
Serotype O ◽  
Fmdv Serotypes ◽  
Virus Serotype ◽  
Foot And Mouth

Background and Aim: Foot-and-mouth disease (FMD) is endemic in several developing countries and affects poor farmers through loss of production, death of diseased animals, and loss of animal byproducts. Forty-three samples were collected from 12 sites of five geographical located areas from suspected FMD virus (FMDV)-infected cattle during 2018. This study aimed to isolate and characterize the FMDVs using reverse transcription-polymerase chain reaction (RT-PCR) and gene sequencing. Materials and Methods: Forty-three FMDV-suspected clinical samples cultured on BHK-21 cell were examined, followed by virus serotype identification using RT-PCR and gene sequencing. Results: Twenty-nine (67.44%) samples were cultured on BHK-21 cell, of which 14 (32.56%) were not isolated; the 43 samples were analyzed using FMDV screening primers and serotype-specific primers. The contribution of the disease-causing serotype was serotype O of 8 (18.60%) samples, serotype A of 20 (46.51%) samples, and mixed infection (O and A) of 1 (2.33%) sample. Serotypes O and A were further characterized by phylogenetic analysis, which grouped them under East Africa 3 and Africa topotypes of genotype IV, respectively. Interestingly, serotype A was isolated for the 1st time from Keyet sub-woreda and Mulo woreda of Ethiopia, and mixed serotypes (O and A) were identified from the purchased animal. Conclusion: Molecular test result, sequencing, and phylogenetic tree reconstruction analysis revealed that the 2018 FMD outbreak in Ethiopia was caused by FMDV serotypes O and A. FMDV serotype A was the predominant strain circulating in most study areas of the country. Infections in one sample with mixed serotypes of O and A were also reported. The authors recommend a vaccine matching study of those field isolated viruses with the vaccine strain.

scholarly journals Cell culture adaptation mutations in foot-and-mouth disease virus serotype A capsid proteins: implications for receptor interactions

2015 ◽  
Vol 96 (3) ◽  
pp. 553-564 ◽  
Author(s):  
Jajati K. Mohapatra ◽  
Laxmi K. Pandey ◽  
Devendra K. Rai ◽  
Biswajit Das ◽  
Luis L. Rodriguez ◽  
...  
Keyword(s):  
Cell Culture ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Capsid Proteins ◽  
Serotype A ◽  
Mouth Disease Virus ◽  
Virus Serotype ◽  
Receptor Interactions ◽  
Foot And Mouth

scholarly journals Generation of mAbs to foot–and–mouth disease virus serotype A and application in a competitive ELISA for serodiagnosis

2016 ◽  
Vol 13 (1) ◽  
Author(s):  
Ming Yang ◽  
Wanhong Xu ◽  
Hilary Bittner ◽  
Jacquelyn Horsington ◽  
Wilna Vosloo ◽  
...  
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Competitive Elisa ◽  
Mouth Disease ◽  
Serotype A ◽  
Mouth Disease Virus ◽  
Virus Serotype ◽  
Foot And Mouth

scholarly journals Molecular detection, phylogenetic analysis and genetic diversity of recently isolated foot-and-mouth disease virus serotype A African topotype, Genotype IV

2022 ◽  
Vol 19 (1) ◽  
Author(s):  
Ayah M. Hassan ◽  
Mostafa R. Zaher ◽  
Rabab T. Hassanien ◽  
Mervat I. Abd-El-Moniem ◽  
Ahmed R. Habashi ◽  
...  
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Emerging Diseases ◽  
Mouth Disease ◽  
Control Measures ◽  
Close Monitoring ◽  
Serotype A ◽  
Mouth Disease Virus ◽  
Virus Serotype ◽  
Foot And Mouth

Abstract Background Surveillance for circulating emerging diseases of economic importance has a major role in the rapid response to major pathogen outbreaks. Foot-and-mouth disease virus (FMDV) is one of the significant endemic viruses in Egypt. FMDV is periodically investigated for monitoring evolution and emergence of new variants. The genetic characterization of foot-and-mouth disease (FMD) virus serotype A responsible for recent outbreaks of FMD in Egypt was determined. Methods Samples were collected from different locations and virus isolation was performed using BHK-21 cells. Viral RNA was extracted and samples were screened for FMDV using real-time RT-PCR. DNA sequence analysis was performed and computational and bioinformatics analyses were used to determine the substitution rates and phylogenetic relationship. Results Sequence and phylogenetic analyses of full-length 1D region of FMDV samples collected from different governorates in 2020 showed close similarity to Egyptian FMDV strains from serotype A-African topotype-G-IV with genetic variation of 6.5%. Recently isolated FMDV strains showed high genetic variations from locally used vaccine strains in the major antigenic sites of VP1 region. Conclusions Although, efforts made by the veterinary authorities to implement an effective mass vaccination plan, the recently detected FMDV strains in this study could not be subtyped using the FMDV primers routinely used for molecular serotyping. These dissimilarities raise the alarm for reconsideration of the FMDV isolates used in vaccine manufacture. Clearly close monitoring of FMD in Egypt is urgently required to define the risks of future outbreaks and to ensure appropriate control measures against FMD major outbreaks.

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