A tailored reverse transcription loop-mediated isothermal amplification for sensitive and specific detection of serotype A foot-and-mouth disease virus circulating in pool 1 region countries

2018 ◽  
Vol 65 (6) ◽  
pp. 1898-1908 ◽  
Author(s):  
Da-Rae Lim ◽  
Hye-Ryung Kim ◽  
Min-Ji Park ◽  
Ha-Gyeong Chae ◽  
Bok-Kyung Ku ◽  
...  
Keyword(s):  
Disease Virus ◽  
Reverse Transcription ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Isothermal Amplification ◽  
Specific Detection ◽  
Serotype A ◽  
Loop Mediated Isothermal Amplification ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Highly Specific Loop-Mediated Isothermal Amplification Using Graphene Oxide–Gold Nanoparticles Nanocomposite for Foot-and-Mouth Disease Virus Detection

Nanomaterials ◽  
2022 ◽  
Vol 12 (2) ◽  
pp. 264
Author(s):  
Jong-Won Kim ◽  
Kyoung-Woo Park ◽  
Myeongkun Kim ◽  
Kyung Kwan Lee ◽  
Chang-Soo Lee
Keyword(s):  
Gold Nanoparticles ◽  
Graphene Oxide ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
High Sensitivity ◽  
Mouth Disease ◽  
Isothermal Amplification ◽  
Specific Detection ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Loop-mediated isothermal amplification (LAMP) is a molecular diagnosis technology with the advantages of rapid results, isothermal reaction conditions, and high sensitivity. However, this diagnostic system often produces false positive results due to a high rate of non-specific reactions caused by formation of hairpin structures, self-dimers, and mismatched hybridization. The non-specific signals can be due to primers used in the methods because the utilization of multiple LAMP primers increases the possibility of self-annealing of primers or mismatches between primers and templates. In this study, we report a nanomaterial-assisted LAMP method that uses a graphene oxide–gold nanoparticles (AuNPs@GO) nanocomposite to enable the detection of foot-and-mouth disease virus (FMDV) with high sensitivity and specificity. Foot-and-mouth disease (FMD) is a highly contagious and deadly disease in cloven-hoofed animals; hence, a rapid, sensitive, and specific detection method is necessary. The proposed approach exhibited high sensitivity and successful reduction of non-specific signals compared to the traditionally established LAMP assays. Additionally, a mechanism study revealed that these results arose from the adsorption of single-stranded DNA on AuNPs@GO nanocomposite. Thus, AuNPs@GO nanocomposite is demonstrated to be a promising additive in the LAMP system to achieve highly sensitive and specific detection of diverse diseases, including FMD.

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