Sensitivity, specificity and predictive probability values of serum agglutination test titres for the diagnosis ofSalmonellaDublin culture-positive bovine abortion and stillbirth

2017 ◽  
Vol 65 (3) ◽  
pp. 676-686 ◽  
Author(s):  
C. Sánchez-Miguel ◽  
J. Crilly ◽  
J. Grant ◽  
J. F. Mee
Keyword(s):  
Agglutination Test ◽  
Predictive Probability ◽  
Sensitivity Specificity ◽  
Bovine Abortion ◽  
Culture Positive ◽  
Serum Agglutination

scholarly journals Value of Fluorescence Polarisation Assay in Comparison to Traditional Techniques in Diagnosis of Porcine Brucellosis

2012 ◽  
Vol 56 (4) ◽  
pp. 467-471 ◽  
Author(s):  
Marcin Weiner ◽  
Wojciech Iwaniak ◽  
Krzysztof Szulowski
Keyword(s):  
False Positive ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Agglutination Test ◽  
Specific Antibodies ◽  
Serological Examination ◽  
Culture Positive ◽  
Serum Agglutination ◽  
Fluorescence Polarisation ◽  
Porcine Brucellosis

Abstract The aim of this study was the evaluation of fluorescence polarisation assay (FPA) in the diagnosis of porcine brucellosis in comparison with Rose Bengal test (RBT), serum agglutination test (SAT), complement fixation test (CFT), 2-mercaptoethanol test, and ELISA. Eight hundred seventeen sera from pigs, including 612 sera from healthy animals, seven sera from Brucella suis bv2 culture positive animals, and 198 sera classified as false positive, originated from confirmatory investigations, were used. All sera from healthy animals, negative in RBT, SAT, CFT, and ELISA were also negative in FPA. All sera positive in serological examination, originated from Brucella infected animals, were also positive in FPA. Among sera classified as false positive almost half of the samples tested (49.49%) reacted positively in FPA. The examinations confirmed the usefulness of FPA in diagnosis of porcine brucellosis, but the method, like the other tests, does not allow resolving the problem of discrimination cross-reacting from specific antibodies.

scholarly journals Salmonella dublin abortion in cattle: I. Observations on the serum agglutination test

1973 ◽  
Vol 71 (3) ◽  
pp. 459-469 ◽  
Author(s):  
M. Hinton
Keyword(s):  
Diagnostic Test ◽  
Agglutination Test ◽  
Active Infection ◽  
Salmonella Dublin ◽  
Paired Samples ◽  
Agglutinin Titre ◽  
Bovine Abortion ◽  
Serum Agglutination ◽  
Specific Diagnostic Test

SUMMARYThe somatic and flagellar serum agglutinin titre were determined in paired samples obtained from seventy-seven cases of bovine abortion associated with Salmonella dublin infection. The cases could be divided into four serological groups with an active infection being demonstrated in most cases. The serum agglutination test was shown to be a relatively specific diagnostic test but was of more limited value in the retrospective identification of convalescent cases.

COMPARISON OF EFFICIENCY OF THE RAPID WHOLE BLOOD AGGLUTINATION TEST WITH THE SERUM AGGLUTINATION TEST FOR PULLORUM DISEASE

1932 ◽  
Vol 6 (4) ◽  
pp. 381-386 ◽  
Author(s):  
Jacob Biely ◽  
William Roach
Keyword(s):  
Laboratory Test ◽  
Whole Blood ◽  
Low Cost ◽  
Agglutination Test ◽  
Practical Application ◽  
Serum Agglutination

The results obtained with the rapid whole blood agglutination test for pullorum disease, applied in the field, agree closely with the results secured with the rapid serum agglutination test, applied in the laboratory.The accuracy of the diagnosis was found to depend upon the training and experience of the technician. When the whole blood agglutination test was applied by inexperienced persons, the results obtained differed from the laboratory test by 12% as compared with a difference of 1.3% when the whole blood agglutination test was applied by an experienced technician.The rapid whole blood agglutination test was found to lend itself very readily to practical application in the field. The extremely low cost makes feasible the application and repetition of the test on a large scale.Since it is known from previous work that one agglutination test will not eliminate all carriers of pullorum disease, the rapid whole blood agglutination test should be applied several times a year until at least two successive negative tests are obtained on each bird of the flock.

A COMPARISON OF THE ROUTINE RAPID WHOLE BLOOD (STAINED ANTIGEN) AND THE ROUTINE RAPID SERUM AGGLUTINATION TESTS FOR PULLORUM DISEASE

1934 ◽  
Vol 10 (6) ◽  
pp. 798-806
Author(s):  
Jacob Biely ◽  
W. Roach
Keyword(s):  
Whole Blood ◽  
Agglutination Test ◽  
Test Laboratory ◽  
Post Mortem ◽  
Post Mortem Examination ◽  
Experiment Station ◽  
Commercial Laboratory ◽  
Serum Agglutination

Data are presented on 4,429 birds, comprising eight flocks, which were tested for pullorum disease by the whole blood agglutination test and the rapid serum agglutination test (commercial laboratory). The diagnoses agreed in the cases of 4,046 birds (97.24%) and disagreed in the cases of 122 birds (2.75%).Of the 122 birds, 43 were diagnosed as positive by the whole blood agglutination test and as negative by the rapid serum agglutination test, while 79 were diagnosed as positive by the rapid serum agglutination test and as negative by the whole blood agglutination test.Of the 122 birds, 102 were retested by the whole blood, rapid serum (Laboratory 1), and rapid serum agglutination test (Laboratory 2, (Experiment Station Laboratory)).There was a closer agreement between the diagnoses made on the basis of the whole blood and rapid serum tests (Laboratory 2) than between those made on the basis of the rapid serum (Laboratory 1) and rapid serum (Laboratory 2) tests (71.56% and 62.37% respectively).A detailed study of the retests and post-mortem examination of the 102 birds is presented.

scholarly journals A quantitative comparison of the sensitivity of serological tests for bovine brucellosis to different antibody classes

1976 ◽  
Vol 76 (2) ◽  
pp. 287-298 ◽  
Author(s):  
G. S. Allan ◽  
R. J. Chappel ◽  
P. Williamson ◽  
D. J. McNaught
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Quantitative Comparison ◽  
Agglutination Test ◽  
Bovine Brucellosis ◽  
Serological Tests ◽  
Plate Test ◽  
Specific Antibodies ◽  
The Rose ◽  
Serum Agglutination

SUMMARYBrucella-specific antibodies of different immunoglobulin classes were quantitatively evaluated with respect to their efficiency in serological tests for bovine brucellosis.IgM reacted more efficiently than IgG1and IgG2in both the Rose Bengal plate test and serum agglutination test. The complement fixation test was found to be slightly more sensitive to IgM than to IgG1and did not react to IgG2.IgM was, however, partly inactivated when heated at 60°C. in the presence of serum.

Comparison evaluation between gene Xpert Mtb/Rif and multiplex PCR for rapid diagnosis of mycobacterium tuberculosis

2020 ◽  
pp. 1-11
Author(s):  
Saira Salim ◽  
Wajid Hussain ◽  
Gohar Zaman ◽  
Umer Khurshid ◽  
Luqman Satti ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Multiplex Pcr ◽  
Gold Standard ◽  
Empirical Therapy ◽  
Armed Forces ◽  
Negative Control ◽  
Cross Sectional ◽  
Limited Region ◽  
Sensitivity Specificity ◽  
Culture Positive

Abstract Objective: To evaluate Gene Xpert MTB/RIF and Multiplex PCRfor detection of Mycobacterium tuberculosis and Rifampicin resistance comparing with gold standard MGIT 960. It was cross sectional validation study. Methods: This study had been carried out at Department of Microbiology Armed Forces Institute of Pathology (AFIP), Rawalpindi Pakistan from March 2018 to October 2018.MGIT 960 culture system MTB positive Rifampicin (RIF) resistant and RIF susceptible (negative control) samples were taken. Gene Xpert MTB / RIF assay and Multiplex PCR were applied simultaneously and compared with gold standard MGIT 960. Results: Out of 192 samples evaluated, 84(44%) were culture positive RIF resistant and 108(56%) were culture positive RIF susceptible as negative control. Out of total culture positive RIF resistant, all 84 were found positive for MTB by Gene Xpert MTB /RIF assay and Multiplex PCR method. Gene Xpert MTB/RIF assay detected all 84 RIF culture resistant samples for Rif resistance. Multiplex PCR detected only 44 RIF culture resistant, while remaining 40 did not showed resistance to rpoB gene codon 531 region. Sensitivity, Specificity, PPV and NPV of Gene Xpert MTB/RIF were 100% each respectively. Sensitivity, Specificity, PPV and NPV of Multiplex PCR for detection of RIF resistance were 52%, 100%, 100%, 72% respectively. Conclusion: Molecular detection of MTB and RIF resistant by Gene Xpert MTB/ RIF and Multiplex PCR simultaneously is rapid and cost effective method. Both methods can help clinician to initiate early empirical therapy to patient in resource limited region. Keywords: RIF, Gene Xpert MTB/ RIF, Multiplex PCR.

scholarly journals Urine-Based ELISA for the Detection of Helicobacter pylori IgG Antibody and Comparison with Other Invasive Methods.

1970 ◽  
Vol 4 (1) ◽  
pp. 14-17 ◽  
Author(s):  
Md Din-ul Islam ◽  
Sufi HZ Rahman ◽  
SM Shamsuzzaman ◽  
Naima Muazzaman ◽  
Nasim Ahmed ◽  
...  
Keyword(s):  
Endoscopic Biopsy ◽  
Rapid Urease Test ◽  
Igg Antibody ◽  
Medical College ◽  
Urease Test ◽  
Study Population ◽  
H Pylori ◽  
Sensitivity Specificity ◽  
Culture Positive ◽  
Upper Gastrointestinal

The present study was conducted in the department of Microbiology, Dhaka Medical College, Dhaka during the period of January, 2007 to December, 2007. Urine samples were collected from 86 dyspeptic patients undergoing upper Gastrointestinal Tract (GIT) endoscopy to determine anti-H. pylori IgG antibody by an ELISA method. Gastric biopsy tissues were tested for culture, rapid urease test and H&E/Giemsa stain. Out of 86 endoscopic biopsy specimens, 45 (52.33%) were culture positive, 63 (73.26%) were rapid urease test positive and 64 (74.42%) were H&E/Giemsa stained positive for H. pylori. According to operational standard definition, among the 86 study population, 66 (76.74%) were H. pylori infected, 16 (18.60%) were uninfected and 4 (4.65%) were indeterminate. Among 66 H. pylori infected cases, 63 (95.45%) were urine ELISA positive and among 16 uninfected cases 3 (18.75%) were urine ELISA positive. Out of 86 study population, 66 (76.74%) were urine ELISA positive. The sensitivity, specificity, PPV, NPP and accuracy of urine ELISA were 95.45%, 81.25%, 95.45%, 81.25% and 92.68% respectively. The result of the study shows that H. pylori infection can be rapidly and reliably diagnosed by detecting anti-H. pylori IgG from urine. Key words: H. pylori; ELISA; GIT; IgG. DOI: http://dx.doi.org/10.3329/bjmm.v4i1.8463 BJMM 2011; 4(1): 14-17

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