Phylogenetic Analysis and Functional Characterization of the Influenza A H5N1 PB2 Gene

2015 ◽  
Vol 64 (2) ◽  
pp. 374-388 ◽  
Author(s):  
K. Wei ◽  
X. Liu
Keyword(s):  
Phylogenetic Analysis ◽  
Influenza A ◽  
Functional Characterization

scholarly journals Structural and Functional Characterization of K339T Substitution Identified in the PB2 Subunit Cap-binding Pocket of Influenza A Virus

2013 ◽  
Vol 288 (16) ◽  
pp. 11013-11023 ◽  
Author(s):  
Yong Liu ◽  
Kun Qin ◽  
Geng Meng ◽  
Jinfang Zhang ◽  
Jianfang Zhou ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Functional Characterization ◽  
Binding Pocket

scholarly journals Structural and functional characterization of neuraminidase-like molecule N10 derived from bat influenza A virus

2012 ◽  
Vol 109 (46) ◽  
pp. 18897-18902 ◽  
Author(s):  
Q. Li ◽  
X. Sun ◽  
Z. Li ◽  
Y. Liu ◽  
C. J. Vavricka ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Functional Characterization

scholarly journals Squalene Cyclases and Cycloartenol Synthases from Polystichum polyblepharum and Six Allied Ferns

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 1843 ◽  
Author(s):  
Junichi Shinozaki ◽  
Takahisa Nakene ◽  
Akihito Takano
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Markers ◽  
Pichia Pastoris ◽  
Vascular Plants ◽  
Type Species ◽  
Functional Characterization ◽  
Flowering Plants ◽  
Biosynthetic Enzymes ◽  
Oxidosqualene Cyclase

Ferns are the most primitive of all vascular plants. One of the characteristics distinguishing them from flowering plants is its triterpene metabolism. Most cyclic triterpenes in ferns are hydrocarbons derived from the direct cyclization of squalene by squalene cyclases (SCs). Both ferns and more complex plants share sterols and biosynthetic enzymes, such as cycloartenol synthases (CASs). Polystichum belongs to Dryopteridaceae, and is one of the most species-rich of all fern genera. Several Polystichum ferns in Japan are classified as one of three possible chemotypes, based on their triterpene profiles. In this study, we describe the molecular cloning and functional characterization of cDNAs encoding a SC (PPH) and a CAS (PPX) from the type species Polystichum polyblepharum. Heterologous expression in Pichia pastoris revealed that PPH and PPX are hydroxyhopane synthase and CAS, respectively. By using the PPH and PPX sequences, we successfully isolated SC- and CAS-encoding cDNAs from six Polystichum ferns. Phylogenetic analysis, based on SCs and oxidosqualene cyclase sequences, suggested that the Polystichum subclade in the fern SC and CAS clades reflects the chemotype—but not the molecular phylogeny constructed using plastid molecular markers. These results show a possible relation between triterpenes and their biosynthetic enzymes in Polystichum.

scholarly journals Phylogenetic Analysis and Characterization of a Sporadic Isolate of Equine Influenza A H3N8 from an Unvaccinated Horse in 2015

Viruses ◽  
2018 ◽  
Vol 10 (1) ◽  
pp. 31 ◽  
Author(s):  
Chithra Sreenivasan ◽  
Sunayana Jandhyala ◽  
Sisi Luo ◽  
Ben Hause ◽  
Milton Thomas ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Influenza A ◽  
Equine Influenza ◽  
Sporadic Isolate

scholarly journals Mechanistic insights from functional characterization of an unnatural His37 mutant of the influenza A/M2 protein

2014 ◽  
Vol 1838 (4) ◽  
pp. 1082-1087 ◽  
Author(s):  
Alexei L. Polishchuk ◽  
Lidia Cristian ◽  
Lawrence H. Pinto ◽  
James D. Lear ◽  
William F. DeGrado
Keyword(s):  
Influenza A ◽  
Functional Characterization ◽  
M2 Protein
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