G-Protein-Coupled Chemokine Receptor Gene in Lumpy Skin Disease Virus Isolates from Cattle and Water Buffalo (Bubalus bubalis) in Egypt

2015 ◽  
Vol 63 (6) ◽  
pp. e288-e295 ◽  
Author(s):  
M. El-Tholoth ◽  
A. A. El-Kenawy
Keyword(s):  
G Protein ◽  
Chemokine Receptor ◽  
Skin Disease ◽  
Water Buffalo ◽  
Receptor Gene ◽  
Bubalus Bubalis ◽  
Lumpy Skin Disease ◽  
G Protein Coupled ◽  
Virus Isolates ◽  
Chemokine Receptor Gene

scholarly journals Capripoxvirus G-protein-coupled chemokine receptor: a host-range gene suitable for virus animal origin discrimination

2009 ◽  
Vol 90 (8) ◽  
pp. 1967-1977 ◽  
Author(s):  
Christian Le Goff ◽  
Charles Euloge Lamien ◽  
Emna Fakhfakh ◽  
Amélie Chadeyras ◽  
Elexpeter Aba-Adulugba ◽  
...  
Keyword(s):  
Host Range ◽  
G Protein ◽  
Chemokine Receptor ◽  
Skin Disease ◽  
Animal Origin ◽  
Lumpy Skin Disease ◽  
Gpcr Gene ◽  
Genetic Clusters ◽  
Host Origin ◽  
Group Diversity

The genus Capripoxvirus within the family Poxviridae comprises three closely related viruses, namely goat pox, sheep pox and lumpy skin disease viruses. This nomenclature is based on the animal species from which the virus was first isolated, respectively, goat, sheep and cattle. Since capripoxviruses are serologically identical, their specific identification relies exclusively on the use of molecular tools. We describe here the suitability of the G-protein-coupled chemokine receptor (GPCR) gene for use in host-range grouping of capripoxviruses. The analysis of 58 capripoxviruses showed three tight genetic clusters consisting of goat pox, sheep pox and lumpy skin disease viruses. However, a few discrepancies exist with the classical virus–host origin nomenclature: a virus isolated from sheep is grouped in the goat poxvirus clade and vice versa. Intra-group diversity was further observed for the goat pox and lumpy skin disease virus isolates. Despite the presence of nine vaccine strains, no genetic determinants of virulence were identified on the GPCR gene. For sheep poxviruses, the addition or deletion of 21 nucleic acids (7 aa) was consistently observed in the 5′ terminal part of the gene. Specific signatures for each cluster were also identified. Prediction of the capripoxvirus GPCR topology, and its comparison with other known mammalian GPCRs and viral homologues, revealed not only a classical GPCR profile in the last three-quarters of the protein but also unique features such as a longer N-terminal end with a proximal hydrophobic α-helix and a shorter serine-rich C-tail.

scholarly journals Human Cytomegalovirus Chemokine Receptor Gene US28 Is Transcribed in Latently Infected THP-1 Monocytes

2001 ◽  
Vol 75 (13) ◽  
pp. 5949-5957 ◽  
Author(s):  
Patrick S. Beisser ◽  
Lysiane Laurent ◽  
Jean-Louis Virelizier ◽  
Susan Michelson
Keyword(s):  
Human Cytomegalovirus ◽  
Immune Evasion ◽  
Chemokine Receptor ◽  
Hcmv Infection ◽  
Receptor Gene ◽  
Cell Types ◽  
Latently Infected ◽  
G Protein Coupled ◽  
Chemokine Receptor Gene

ABSTRACT The human cytomegalovirus (HCMV) US28 gene product, pUS28, is a G protein-coupled receptor that interacts with both CC and CX3C chemokines. To date, the role of pUS28 in immune evasion and cell migration has been studied only in cell types that can establish productive HCMV infection. We show that HCMV can latently infect THP-1 monocytes and that during latency US28 is transcribed. We also show that the transcription is sustained during differentiation of the THP-1 monocytes. Since cells expressing pUS28 were previously shown to adhere to immobilized CX3C chemokines (C. A. Haskell, M. D. Cleary, and I. F. Charo, J. Biol. Chem. 275:34183–34189, 2000), we hypothesize that latently infected circulating monocytes express pUS28, thereby enabling adhesion of these cells to CX3C-exposing endothelium. Consequently, the US28-encoded chemokine receptor may play an important role in dissemination of latent HCMV.

scholarly journals The G Protein-Coupled Receptor Kinases (GRKs) in Chemokine Receptor-Mediated Immune Cell Migration: From Molecular Cues to Physiopathology

Cells ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 75
Author(s):  
Marta Laganà ◽  
Géraldine Schlecht-Louf ◽  
Françoise Bachelerie
Keyword(s):  
G Protein ◽  
Chemokine Receptor ◽  
Immune Cell ◽  
Neutrophil Migration ◽  
G Protein Coupled Receptor ◽  
Receptor Kinases ◽  
Immune Cell Migration ◽  
And Migration ◽  
G Protein Coupled ◽  
Gpcr Desensitization

Although G protein-coupled receptor kinases (GRKs) have long been known to regulate G protein-coupled receptor (GPCR) desensitization, their more recently characterized functions as scaffolds and signalling adapters underscore that this small family of proteins governs a larger array of physiological functions than originally suspected. This review explores how GRKs contribute to the complex signalling networks involved in the migration of immune cells along chemokine gradients sensed by cell surface GPCRs. We outline emerging evidence indicating that the coordinated docking of several GRKs on an active chemokine receptor determines a specific receptor phosphorylation barcode that will translate into distinct signalling and migration outcomes. The guidance cues for neutrophil migration are emphasized based on several alterations affecting GRKs or GPCRs reported to be involved in pathological conditions.

scholarly journals Polymorphism of chemokine receptor gene CCR5 in multiple sclerosis patients and in healthy subjects in the Siberian region

2006 ◽  
Vol 5 (3) ◽  
pp. 98-104
Author(s):  
Yu. Yu. Orlova ◽  
V. M. Alifirova ◽  
N. V. Cherdyntseva ◽  
P. A. Gervas
Keyword(s):  
Multiple Sclerosis ◽  
Central Nervous System ◽  
Nervous System ◽  
Chemokine Receptor ◽  
Healthy Subjects ◽  
Receptor Gene ◽  
Chronic Inflammatory Disease ◽  
Leading Role ◽  
Multiple Sclerosis Patients ◽  
Chemokine Receptor Gene

Multiple sclerosis is chronic inflammatory disease of the central nervous system in the development of which chemokines of the type Tx1 play the leading role. Chemokines and their receptors participate in the development of multiple sclerosis as a result of drawing immune cells into central nervous system. Mutation of CCR5 delta32 decreases functional activity of the appropriate receptor on cellular surface and thus can reduce migration of leucocytes into foci of injury. Aimed at studying the role of mutation in multiple sclerosis, we compared frequency of gene type CCR5 in peripheral mononuclears of 102 multiple sclerosis patients and in 136 healthy subjects. The results obtained allow to conclude that polymorphism of chemokine receptor gene CCR5del32 is not a leading factor in the susceptibility to multiple sclerosis in the studied population.

CCR5 chemokine receptor gene evolution in New World monkeys (Platyrrhini, Primates): implication on resistance to lentiviruses

2005 ◽  
Vol 5 (3) ◽  
pp. 271-280 ◽  
Author(s):  
Ieda P. Ribeiro ◽  
Carlos G. Schrago ◽  
Esmeralda A. Soares ◽  
Alcides Pissinatti ◽  
Hector N. Seuanez ◽  
...  
Keyword(s):  
Chemokine Receptor ◽  
New World ◽  
Gene Evolution ◽  
Receptor Gene ◽  
New World Monkeys ◽  
Ccr5 Chemokine Receptor ◽  
Chemokine Receptor Gene
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