Emergence of Porcine Circovirus 2 Associated Reproductive Failure in Southern India

2014 ◽  
Vol 63 (3) ◽  
pp. 314-320 ◽  
Author(s):  
A. K. Karuppannan ◽  
A. Ramesh ◽  
Y. K. Reddy ◽  
S. Ramesh ◽  
R. Mahaprabhu ◽  
...  
Keyword(s):  
Reproductive Failure ◽  
Southern India ◽  
Porcine Circovirus ◽  
Porcine Circovirus 2

Seroprevalence of Porcine Circovirus-2 Associated Reproductive Failure in Pigs of Mizoram, India

2018 ◽  
pp. 1
Author(s):  
Laltlankimi Varte ◽  
Ramanathan Ravindran ◽  
Lalchamliani Chhangte ◽  
Lalsangpuii Tonsing
Keyword(s):  
Reproductive Failure ◽  
Porcine Circovirus ◽  
Porcine Circovirus 2

Emergence of novel Porcine circovirus 2 genotypes in Southern India

2021 ◽  
Author(s):  
S Parthiban ◽  
A Ramesh ◽  
Anbu Kumar Karuppannan ◽  
G Dhinakar Raj ◽  
J Johnson Rajeswar ◽  
...  
Keyword(s):  
Southern India ◽  
Porcine Circovirus ◽  
Porcine Circovirus 2

scholarly journals A Novel Porcine Circovirus Distantly Related to Known Circoviruses Is Associated with Porcine Dermatitis and Nephropathy Syndrome and Reproductive Failure

2016 ◽  
Vol 91 (1) ◽  
Author(s):  
Rachel Palinski ◽  
Pablo Piñeyro ◽  
Pengcheng Shang ◽  
Fangfeng Yuan ◽  
Rui Guo ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Postweaning Multisystemic Wasting Syndrome ◽  
Reproductive Failure ◽  
Porcine Circovirus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Metagenomic Sequencing ◽  
Tissue Samples ◽  
Wasting Syndrome ◽  
Etiologic Role ◽  
Porcine Circovirus 2

ABSTRACT Porcine circovirus-associated disease (PCVAD) is clinically manifested by postweaning multisystemic wasting syndrome (PMWS), respiratory and enteric disease, reproductive failure, and porcine dermatitis and nephropathy syndrome (PDNS). Porcine circovirus 2 (PCV2) is an essential component of PCVAD, although an etiologic role in PDNS is not well established. Here, a novel circovirus, designated porcine circovirus 3 (PCV3), was identified in sows that died acutely with PDNS-like clinical signs. The capsid and replicase proteins of PCV3 are only 37% and 55% identical to PCV2 and bat circoviruses, respectively. Aborted fetuses from sows with PDNS contained high levels of PCV3 (7.57 × 107 genome copies/ml), and no other viruses were detected by PCR and metagenomic sequencing. Immunohistochemistry (IHC) analysis of sow tissue samples identified PCV3 antigen in skin, kidney, lung, and lymph node samples localized in typical PDNS lesions, including necrotizing vasculitis, glomerulonephritis, granulomatous lymphadenitis, and bronchointerstitial pneumonia. Further study of archived PDNS tissue samples that were negative for PCV2 by IHC analysis identified 45 of 48 that were PCV3 positive by quantitative PCR (qPCR), with 60% of a subset also testing positive for PCV3 by IHC analysis. Analysis by qPCR of 271 porcine respiratory disease diagnostic submission samples identified 34 PCV3-positive cases (12.5%), and enzyme-linked immunosorbent assay detection of anti-PCV3 capsid antibodies in serum samples found that 46 (55%) of 83 samples tested were positive. These results suggest that PCV3 commonly circulates within U.S. swine and may play an etiologic role in reproductive failure and PDNS. Because of the high economic impact of PCV2, this novel circovirus warrants further studies to elucidate its significance and role in PCVAD. IMPORTANCE While porcine circovirus 2 (PCV2) was first identified in sporadic cases of postweaning multisystemic wasting syndrome in Canada in the early 1990s, an epidemic of severe systemic disease due to PCV2 spread worldwide in the ensuing decade. Despite being effectively controlled by commercial vaccines, PCV2 remains one of the most economically significant viruses of swine. Here, a novel porcine circovirus (PCV3) that is distantly related to known circoviruses was identified in sows with porcine dermatitis and nephropathy syndrome (PDNS) and reproductive failure. PCV2, which has previously been associated with these clinical presentations, was not identified. High levels of PCV3 nucleic acid were observed in aborted fetuses by quantitative PCR, and PCV3 antigen was localized in histologic lesions typical of PDNS in sows by immunohistochemistry (IHC) analysis. PCV3 was also identified in archival PDNS diagnostic samples that previously tested negative for PCV2 by IHC analysis. The emergence of PCV3 warrants further investigation.

scholarly journals Exploratory field study on the effects of porcine circovirus 2 (PCV-2) sow vaccination at different physiological stages mimicking blanket vaccination

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Patricia Pleguezuelos ◽  
Marina Sibila ◽  
Raúl Cuadrado ◽  
Rosa López-Jiménez ◽  
Diego Pérez ◽  
...  
Keyword(s):  
Quantitative Polymerase Chain Reaction ◽  
Negative Control Group ◽  
Negative Control ◽  
Late Gestation ◽  
Antibody Detection ◽  
Porcine Circovirus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Physiological Status ◽  
Porcine Circovirus 2

Abstract Background The objective of the present study was to explore the benefits of Porcine circovirus 2 (PCV-2) blanket vaccination in a sow herd on productive parameters, PCV-2 infection and immune status in sows and their progeny. For this purpose, 288 sows were distributed among four balanced experimental groups. One group remained as negative control group and the other three received 1 mL of PCV-2 Ingelvac Circoflex® intramuscularly at different productive cycle moments: before mating, mid gestation (42–49 days post-insemination) or late gestation (86–93 days post-insemination); phosphate buffered saline (PBS) was used as negative control item. Reproductive parameters from sows during gestation and body weight of their progeny from birth to weaning were recorded. Additionally, blood was collected from sows at each vaccination time and piglets at 3 weeks of age. Moreover, up to 4 placental umbilical cords (PUC) per sow were taken at peri-partum. Sera from sows and piglets were analysed for PCV-2 antibody detection using an enzyme-linked immunosorbent assay (ELISA). Sera from sows and PUC were tested to quantify viraemia using a real time quantitative polymerase chain reaction (qPCR) assay. Results Globally, results indicated that vaccinated sows showed heavier piglets at birth and at weaning, less cross-fostered piglets, lower viral load at farrowing as well as in PUC, and higher antibody levels at farrowing, compared to non-vaccinated ones. When all groups were compared among them, sows vaccinated at mid or late gestation had heavier piglets at birth than non-vaccinated sows, and lower proportion of PCV-2 positive PUC. Also, cross-fostering was less frequently practiced in sows vaccinated at pre-mating or mid gestation compared to non-vaccinated ones. Conclusions In conclusion, the present study points out that PCV-2 sow vaccination at different time points of their physiological status (mimicking blanket vaccination) offers benefits at production and serological and virological levels.

Correlates of Protection Following Vaccination with Inactivated Porcine Circovirus 2 Vaccines

2015 ◽  
Vol 28 (10) ◽  
pp. 600-608 ◽  
Author(s):  
Cinzia Zanotti ◽  
Nicola Martinelli ◽  
Davide Lelli ◽  
Massimo Amadori
Keyword(s):  
Porcine Circovirus ◽  
Correlates Of Protection ◽  
Porcine Circovirus 2

Cell tropism and entry of porcine circovirus 2

2012 ◽  
Vol 164 (1-2) ◽  
pp. 43-45 ◽  
Author(s):  
H.J. Nauwynck ◽  
R. Sanchez ◽  
P. Meerts ◽  
D.J. Lefebvre ◽  
D. Saha ◽  
...  
Keyword(s):  
Porcine Circovirus ◽  
Cell Tropism ◽  
Porcine Circovirus 2
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