Inside the pocket: critical elements of HLA ‐mediated susceptibility to cervical precancerous lesions

HLA ◽  
2021 ◽  
Author(s):  
Letícia Boslooper Gonçalves ◽  
Patrícia Pinho França ◽  
Natália Angelica Petry ◽  
Marina Bárbara de Souza Xavier ◽  
Newton Sérgio Carvalho ◽  
...  
Keyword(s):  
Precancerous Lesions ◽  
Critical Elements ◽  
Cervical Precancerous Lesions

scholarly journals Clinical application of the 2011 IFCPC colposcope terminology

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Bei Zhang ◽  
Shuhui Hong ◽  
Guihui Zhang ◽  
Fengnian Rong
Keyword(s):  
Diagnostic Accuracy ◽  
Precancerous Lesions ◽  
High Specificity ◽  
Diagnostic Value ◽  
Low Grade ◽  
Squamous Intraepithelial Lesion ◽  
Diagnostic Significance ◽  
Cervical Precancerous Lesions ◽  
Intraepithelial Lesion ◽  
Common Sign

Abstract Background Colposcopy offers an accurate way to the diagnose of cervical precancerous lesions. However, the diagnostic accuracy of colposcopy is unsatisfied. This study was to evaluate colposcopic accuracy according to the 2011 International Federation of Cervical Pathology and Colposcopy (IFCPC) terminology. Methods A retrospective cohort study was performed in 1,838 patients who underwent colposcopy in Shandong Qianfoshan Hospital, Cheeloo College of Medicine, Shandong University from October 2013 to April 2018. Using conization or cervical biopsy pathology as the gold standard, the agreement between colposcopic diagnosis and pathologic diagnosis was calculated, and correlations between variables were analyzed. Results As an authoritative and widely used terminology for colposcopy diagnosis, the 2011 IFCPC terminology has certain clinical practicality and diagnostic accuracy. However, some signs such as mosaic, punctation, sharp border, inner border sign and ridge sign had high specificity but unsatisfactory sensitivity, which limited the diagnostic value. Therefore, we discussed the Lugol’s staining, a very common sign in colposcopy, and analyzed the diagnostic significance of bright yellow staining in low-grade squamous intraepithelial lesion (LSIL) and mustard yellow staining in high-grade squamous intraepithelial lesion (HSIL). The results showed that mustard yellow may be a valuable indicator in the diagnosis of HSIL. Conclusion The 2011 IFCPC colposcope terminology has standardized interpretations of the colposcopic findings and improved the accuracy of colposcopy diagnosis. The aceto-white epithelium still has important diagnostic value; however, the value of a few signs is needed to be discussed and new signs are expected to be discovered. Although the significance of Lugol’s staining was diminishing, mustard yellow might be a valuable indicator for the diagnosis of HSIL.

scholarly journals The application of CRISPR/Cas9 system in cervical carcinogenesis

2021 ◽  
Author(s):  
Chun Gao ◽  
Ping Wu ◽  
Lan Yu ◽  
Liting Liu ◽  
Hong Liu ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Transgenic Mice ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Precancerous Lesions ◽  
Cancer Cell Lines ◽  
Cervical Carcinogenesis ◽  
Cervical Precancerous Lesions

AbstractIntegration of high-risk HPV genomes into cellular chromatin has been confirmed to promote cervical carcinogenesis, with HPV16 being the most prevalent high-risk type. Herein, we evaluated the therapeutic effect of the CRISPR/Cas9 system in cervical carcinogenesis, especially for cervical precancerous lesions. In cervical cancer/pre-cancer cell lines, we transfected the HPV16 E7 targeted CRISPR/Cas9, TALEN, ZFN plasmids, respectively. Compared to previous established ZFN and TALEN systems, CRISPR/Cas9 has shown comparable efficiency and specificity in inhibiting cell growth and colony formation and inducing apoptosis in cervical cancer/pre-cancer cell lines, which seemed to be more pronounced in the S12 cell line derived from the low-grade cervical lesion. Furthermore, in xenograft formation assays, CRISPR/Cas9 inhibited tumor formation of the S12 cell line in vivo and affected the corresponding protein expression. In the K14-HPV16 transgenic mice model of HPV-driven spontaneous cervical carcinogenesis, cervical application of CRISPR/Cas9 treatment caused mutations of the E7 gene and restored the expression of RB, E2F1, and CDK2, thereby reversing the cervical carcinogenesis phenotype. In this study, we have demonstrated that CRISPR/Cas9 targeting HPV16 E7 could effectively revert the HPV-related cervical carcinogenesis in vitro, as well as in K14-HPV16 transgenic mice, which has shown great potential in clinical treatment for cervical precancerous lesions.

scholarly journals Raman spectroscopic study of cervical precancerous lesions and cervical cancer

2021 ◽  
Author(s):  
Jing Wang ◽  
Cheng-Xia Zheng ◽  
Cai-Ling Ma ◽  
Xiang-Xiang Zheng ◽  
Xiao-Yi Lv ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Squamous Cell Carcinoma ◽  
Raman Spectra ◽  
Precancerous Lesions ◽  
Cervical Squamous Cell Carcinoma ◽  
Cervical Lesions ◽  
Cin Iii ◽  
Cervical Precancerous Lesions ◽  
Biochemical Components ◽  
The Difference

AbstractEarly detection of cervical lesions, accurate diagnosis of cervical lesions, and timely and effective therapy can effectively avoid the occurrence of cervical cancer or improve the survival rate of patients. In this paper, the spectra of tissue sections of cervical inflammation (n = 60), CIN (cervical intraepithelial neoplasia) I (n = 30), CIN II (n = 30), CIN III (n = 30), cervical squamous cell carcinoma (n = 30), and cervical adenocarcinoma (n = 30) were collected by a confocal Raman micro-spectrometer (LabRAM HR Evolution, Horiba France SAS, Villeneuve d’Ascq, France). The Raman spectra of six kinds of cervical tissues were analyzed, the dominant Raman peaks of different kinds of tissues were summarized, and the differences in chemical composition between the six tissue samples were compared. An independent sample t test (p ≤ 0.05) was used to analyze the difference of average relative intensity of Raman spectra of six types of cervical tissues. The difference of relative intensity of Raman spectra of six kinds of tissues can reflect the difference of biochemical components in six kinds of tissues and the characteristic of biochemical components in different kinds of tissues. The classification models of cervical inflammation, CIN I, CIN II, CIN III, cervical squamous cell carcinoma, and cervical adenocarcinoma were established by using a support vector machine (SVM) algorithm. Six types of cervical tissues were classified and identified with an overall diagnostic accuracy of 85.7%. This study laid a foundation for the application of Raman spectroscopy in the clinical diagnosis of cervical precancerous lesions and cervical cancer.

scholarly journals Distribution and attribution of high-risk human papillomavirus genotypes in cervical precancerous lesions in China

Tumor Biology ◽  
2017 ◽  
Vol 39 (7) ◽  
pp. 101042831770737 ◽  
Author(s):  
Wenpeng Wang ◽  
Jusheng An ◽  
Yan Song ◽  
Minjie Wang ◽  
Manni Huang ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Precancerous Lesions ◽  
Cervical Precancerous Lesions

Confocal microscopy: Imaging cervical precancerous lesions

2005 ◽  
Vol 99 (3) ◽  
pp. S84-S88 ◽  
Author(s):  
Kristen Carlson ◽  
Ina Pavlova ◽  
Tom Collier ◽  
Michael Descour ◽  
Michele Follen ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Precancerous Lesions ◽  
Microscopy Imaging ◽  
Cervical Precancerous Lesions ◽  
Confocal Microscopy Imaging

scholarly journals MobileNetV2 Ensemble for Cervical Precancerous Lesions Classification

Processes ◽  
2020 ◽  
Vol 8 (5) ◽  
pp. 595 ◽  
Author(s):  
Cătălin Buiu ◽  
Vlad-Rareş Dănăilă ◽  
Cristina Nicoleta Răduţă
Keyword(s):  
Uterine Cervix ◽  
Precancerous Lesions ◽  
Binary Classification ◽  
The United States ◽  
Medical Procedure ◽  
Medical Doctors ◽  
Analysis Framework ◽  
Cervical Precancerous Lesions ◽  
Classification Tasks

Women’s cancers remain a major challenge for many health systems. Between 1991 and 2017, the death rate for all major cancers fell continuously in the United States, excluding uterine cervix and uterine corpus cancers. Together with HPV (Human Papillomavirus) testing and cytology, colposcopy has played a central role in cervical cancer screening. This medical procedure allows physicians to view the cervix at a magnification of up to 10%. This paper presents an automated colposcopy image analysis framework for the classification of precancerous and cancerous lesions of the uterine cervix. This framework is based on an ensemble of MobileNetV2 networks. Our experimental results show that this method achieves accuracies of 83.33% and 91.66% on the four-class and binary classification tasks, respectively. These results are promising for the future use of automatic classification methods based on deep learning as tools to support medical doctors.

Role of human papillomavirus (HPV) testing in the follow-up of patients after treatment for cervical precancerous lesions

2005 ◽  
Vol 118 (2) ◽  
pp. 229-234 ◽  
Author(s):  
Zoltán Hernádi ◽  
Krisztina Szőke ◽  
Tamás Sápy ◽  
Zoárd T. Krasznai ◽  
Györgyike Soós ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Precancerous Lesions ◽  
Hpv Testing ◽  
Cervical Precancerous Lesions

Evaluation of the LC-1000 Flow Cytometry Screening System for Cervical Cancer Screening in Routine Health Checkups

2018 ◽  
Vol 62 (4) ◽  
pp. 279-287
Author(s):  
Ilyeong Heo ◽  
Hyoung-Jung Kwak ◽  
Eun-Hee Nah ◽  
Seon Cho ◽  
Suyoung Kim ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Precancerous Lesions ◽  
Cervical Cytology ◽  
Proliferation Index ◽  
Low Grade ◽  
Squamous Intraepithelial Lesion ◽  
Normal Cytology ◽  
Cervical Precancerous Lesions ◽  
Intraepithelial Lesion ◽  
Sensitivity Specificity

Objective: This study evaluated the performance of a flow cytometry system (LC-1000) in screening cervical precancerous lesions at routine health checkups. Study design: In total, 928 health examinees were enrolled at 16 health promotion centers in 13 Korean cities between 2016 and 2017. All participants underwent liquid-based cervical cytology and flow cytometry testing to determine the cell proliferation index (CPIx). Results: The positivity rate of the LC-1000 system increased with the severity of the cervical cytology findings (p for trend < 0.001). When low-grade squamous intraepithelial lesion (LSIL) or higher (including LSIL, high-grade squamous intraepithelial lesion [HSIL], and atypical squamous cells without excluding HSIL [ASC-H]) was defined as gold-standard positivity, the sensitivity, specificity, PPV, and NPV of LC-1000 were 75.3% (95% confidence interval [CI], 66.8–83.7), 58.5% (95% CI, 55.2–61.9), 18.1% (95% CI, 14.5–21.8), and 95.1% [95% CI, 93.2–97.0], respectively. The median CPIx increased significantly from normal cytology to HSIL (p < 0.001). The median CPIx was higher in high-risk human papillomavirus (HR-HPV)-positive cases than in HR-HPV-negative cases (0.23 vs. 0.17, p < 0.001), while it did not differ between HR-HPV-positive and HR-HPV-negative cases with normal cytology findings (0.16 vs. 0.16, p = 0.700). Conclusion: The LC-1000 system is potentially useful for screening cervical precancer and cancer, especially when excluding normal or ASC of undetermined significance cases in routinely screened populations.

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