Comparison of two peripheral mononuclear cell isolation protocols for flow cytometry crossmatching

HLA ◽  
2018 ◽  
Vol 91 (2) ◽  
pp. 124-126 ◽  
Author(s):  
M.-J. Apithy ◽  
J. Desoutter ◽  
N. Guillaume
Keyword(s):  
Flow Cytometry ◽  
Mononuclear Cell ◽  
Cell Isolation ◽  
Peripheral Mononuclear Cell

scholarly journals Monocyte CD163 and CD36 Expression in Human Whole Blood and Isolated Mononuclear Cell Samples: Influence of Different Anticoagulants

2006 ◽  
Vol 13 (6) ◽  
pp. 704-707 ◽  
Author(s):  
Marcin Moniuszko ◽  
Krzysztof Kowal ◽  
Malgorzata Rusak ◽  
Miroslawa Pietruczuk ◽  
Milena Dabrowska ◽  
...  
Keyword(s):  
Density Gradient ◽  
Mononuclear Cell ◽  
Whole Blood ◽  
Cell Isolation ◽  
Scavenger Receptors ◽  
Published Data ◽  
Healthy Individuals ◽  
Human Whole Blood ◽  
Cd36 Expression

ABSTRACT We investigated whether the choice of anticoagulant or the application of density gradient mononuclear cell isolation may account for conflicting published data regarding the levels of the scavenger receptors' expression in healthy individuals. We demonstrate that the detection of CD163, but not CD36, differs dramatically among the methods.

461 Prostate stem cell isolation using Hoechst 33342 flow cytometry

2004 ◽  
Vol 3 (2) ◽  
pp. 118
Author(s):  
P. Gilmore ◽  
R. Bhatt ◽  
C. Hart ◽  
V. Ramani ◽  
N. George ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Stem Cell ◽  
Cell Isolation ◽  
Hoechst 33342 ◽  
Stem Cell Isolation

scholarly journals Peripheral Mononuclear Cell Response in Japanese Black Calves after Oral Administration of IFN-α

2006 ◽  
Vol 68 (10) ◽  
pp. 1063-1067
Author(s):  
Hiromichi OHTSUKA ◽  
Mayumi TOKITA ◽  
Katsushige TAKAHASHI ◽  
Machiko MASUI ◽  
Masayuki KOHIRUIMAKI ◽  
...  
Keyword(s):  
Oral Administration ◽  
Mononuclear Cell ◽  
Cell Response ◽  
Peripheral Mononuclear Cell

Detection of HBD1 peptide in peripheral blood mononuclear cell subpopulations by intracellular flow cytometry

Peptides ◽  
2003 ◽  
Vol 24 (11) ◽  
pp. 1785-1794 ◽  
Author(s):  
Lisa K. Ryan ◽  
Gill Diamond ◽  
Sheela Amrute ◽  
Zhimin Feng ◽  
Aaron Weinberg ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Peripheral Blood Mononuclear Cell ◽  
Mononuclear Cell ◽  
Peripheral Blood ◽  
Peripheral Blood Mononuclear ◽  
Cell Subpopulations

scholarly journals NK Cell Isolation from Liver Biopsies: Phenotypic and Functional Analysis of Low Cell Numbers by Flow Cytometry

2013 ◽  
Vol 4 ◽  
Author(s):  
Ning Li ◽  
Gisella L. Puga Yung ◽  
Amandine Pradier ◽  
Christian Toso ◽  
Emiliano Giostra ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Functional Analysis ◽  
Nk Cell ◽  
Cell Isolation ◽  
Cell Numbers ◽  
Liver Biopsies

scholarly journals Circulating adult stem and progenitor cell numbers—can results be trusted?

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Julia M. Kröpfl ◽  
Michelle Schmid ◽  
Yvonne Di Marzio ◽  
Karine Schreiber ◽  
Christina M. Spengler
Keyword(s):  
Flow Cytometry ◽  
Physical Exercise ◽  
Progenitor Cell ◽  
Mononuclear Cells ◽  
Cell Isolation ◽  
Hematopoietic Stem ◽  
Isolation Technique ◽  
Non Invasive ◽  
Cell Counts ◽  
Back Calculation

Abstract Background Within the last years, the interest in physical exercise as non-invasive stimulus influencing circulating hematopoietic stem and progenitor cell (CPC) concentrations has constantly grown. Cell estimates are often derived by determining the subgroup of CPC as percent lymphocytes (LYM) or mononuclear cells (MNC) via flow cytometry and back calculation over whole blood (WB) cell counts. However, results might depend on the used cell isolation technique and/or gating strategy. We aimed to investigate MNC loss and apoptosis during the flow cytometry sample preparation process preceded by either density gradient centrifugation (DGC) or red blood cell lysis (RBCL) and the potential difference between results derived from back calculation at different stages of cell isolation and from WB. Methods Human blood was subjected to DGC and RBCL. Samples were stained for flow cytometry analysis of CPC (CD34+/CD45dim) and apoptosis analysis (Annexin V) of MNC and CPC subsets. MNC and LYM gating strategies were compared. Results Both DGC as well as RBCL yielded comparable CPC concentrations independent of the gating strategy when back calculated over WB values. However, cell loss and apoptosis differed between techniques, where after DGC LYM, and monocyte (MONO) concentrations significantly decreased (p < 0.01 and p < 0.05, respectively), while after RBCL LYM concentrations significantly decreased (p < 0.05) and MONO concentrations increased (p < 0.001). LYM apoptosis was comparable between techniques, but MONO apoptosis was higher after DGC than RBCL (p < 0.001). Conclusions Investigated MNC counts (LYM/MONO ratio) after cell isolation and staining did not always mimic WB conditions. Thus, final CPC results should be corrected accordingly, especially when reporting live CPC concentrations after DGC; otherwise, the CPC regenerative potential in circulation could be biased. This is of high importance in the context of non-invasively induced CPC mobilization such as by acute physical exercise, since these cell changes are small and conclusions drawn from published results might affect further applications of physical exercise as non-invasive therapy.

Peripheral Mononuclear Cell Rejuvenation for Senescence Surveillance in Alzheimer Disease

2013 ◽  
Vol 19 (9) ◽  
pp. 1720-1726
Author(s):  
Marco Malavolta ◽  
Andrea Basso ◽  
Francesco Piacenza ◽  
Laura Costarelli ◽  
Robertina Giacconi ◽  
...  
Keyword(s):  
Alzheimer Disease ◽  
Mononuclear Cell ◽  
Peripheral Mononuclear Cell
Sign in / Sign up

Export Citation Format

Share Document