Sequence identification, serological reactivity and family genetics of a novel HLA allele, HLA-A*26:82

HLA ◽  
2017 ◽  
Vol 90 (5) ◽  
pp. 308-309 ◽  
Author(s):  
C. Zhu ◽  
X. Nie ◽  
Y. Song ◽  
Y. Zhang ◽  
W. Qiao
Keyword(s):  
Sequence Identification ◽  
Serological Reactivity ◽  
Family Genetics

scholarly journals A machine learning approach for accurate and real-time DNA sequence identification

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Yiren Wang ◽  
Mashari Alangari ◽  
Joshua Hihath ◽  
Arindam K. Das ◽  
M. P. Anantram
Keyword(s):  
Real Time ◽  
Dna Sequence ◽  
Single Molecule ◽  
Identification Accuracy ◽  
Measuring System ◽  
Identification System ◽  
Genetic Sequencing ◽  
Sequence Identification ◽  
Tree Classifier ◽  
Single Mismatch

Abstract Background The all-electronic Single Molecule Break Junction (SMBJ) method is an emerging alternative to traditional polymerase chain reaction (PCR) techniques for genetic sequencing and identification. Existing work indicates that the current spectra recorded from SMBJ experimentations contain unique signatures to identify known sequences from a dataset. However, the spectra are typically extremely noisy due to the stochastic and complex interactions between the substrate, sample, environment, and the measuring system, necessitating hundreds or thousands of experimentations to obtain reliable and accurate results. Results This article presents a DNA sequence identification system based on the current spectra of ten short strand sequences, including a pair that differs by a single mismatch. By employing a gradient boosted tree classifier model trained on conductance histograms, we demonstrate that extremely high accuracy, ranging from approximately 96 % for molecules differing by a single mismatch to 99.5 % otherwise, is possible. Further, such accuracy metrics are achievable in near real-time with just twenty or thirty SMBJ measurements instead of hundreds or thousands. We also demonstrate that a tandem classifier architecture, where the first stage is a multiclass classifier and the second stage is a binary classifier, can be employed to boost the single mismatched pair’s identification accuracy to 99.5 %. Conclusions A monolithic classifier, or more generally, a multistage classifier with model specific parameters that depend on experimental current spectra can be used to successfully identify DNA strands.

scholarly journals Sequence identification of cytochrome b in Plasmodium gallinaceum.

1989 ◽  
Vol 9 (9) ◽  
pp. 3614-3620 ◽  
Author(s):  
S M Aldritt ◽  
J T Joseph ◽  
D F Wirth
Keyword(s):  
Cytochrome B ◽  
Open Reading Frame ◽  
Malarial Parasite ◽  
Plasmodium Gallinaceum ◽  
Primary Transcript ◽  
Present Evidence ◽  
Reading Frame ◽  
Sequence Identification ◽  
Parasite Plasmodium ◽  
Extrachromosomal Element

We have identified a gene that encodes the polypeptide cytochrome b in the avian malarial parasite Plasmodium gallinaceum. The gene containing the open reading frame was found to be located on a 6.2-kilobase multimeric extrachromosomal element. The amino acid translation from this gene demonstrated significant similarities to cytochrome b sequences from yeast, mammal, and fungus genomes. We present evidence that the P. gallinaceum cytochrome b transcript is part of a larger primary transcript from the element that is subsequently processed. The message for P. gallinaceum cytochrome b was found to be 1.2 kilobases in size. This is the first report identifying a mitochondrial nucleic acid sequence in malaria-causing organisms and suggests that a functional cytochrome system may exist in these parasites.

scholarly journals Pectobacterium spp. Associated with Bacterial Stem Rot Syndrome of Potato in Canada

2012 ◽  
Vol 102 (10) ◽  
pp. 937-947 ◽  
Author(s):  
S. H. De Boer ◽  
X. Li ◽  
L. J. Ward
Keyword(s):  
Gene Sequence ◽  
Blackleg Disease ◽  
Chain Reaction ◽  
Biochemical Tests ◽  
Pectobacterium Atrosepticum ◽  
Physiological Characterization ◽  
Specific Polymerase Chain Reaction ◽  
Sequence Identification ◽  
Carotovorum Subsp ◽  
Polymerase Chain

Pectobacterium atrosepticum, P. carotovorum subsp. brasiliensis, P. carotovorum subsp. carotovorum, and P. wasabiae were detected in potato stems with blackleg symptoms using species- and subspecies-specific polymerase chain reaction (PCR). The tests included a new assay for P. wasabiae based on the phytase gene sequence. Identification of isolates from diseased stems by biochemical or physiological characterization, PCR, and multi-locus sequence typing (MLST) largely confirmed the PCR detection of Pectobacterium spp. in stem samples. P. atrosepticum was most commonly present but was the sole Pectobacterium sp. detected in only 52% of the diseased stems. P. wasabiae was most frequently present in combination with P. atrosepticum and was the sole Pectobacterium sp. detected in 13% of diseased stems. Pathogenicity of P. wasabiae on potato and its capacity to cause blackleg disease were demonstrated by stem inoculation and its isolation as the sole Pectobacterium sp. from field-grown diseased plants produced from inoculated seed tubers. Incidence of P. carotovorum subsp. brasiliensis was low in diseased stems, and the ability of Canadian strains to cause blackleg in plants grown from inoculated tubers was not confirmed. Canadian isolates of P. carotovorum subsp. brasiliensis differed from Brazilian isolates in diagnostic biochemical tests but conformed to the subspecies in PCR specificity and typing by MLST.

scholarly journals A thaumatin-like genomic sequence identification in Vitis vinifera l., stormy wines and musts based on direct pcr

10.5219/892 ◽  
2018 ◽  
Vol 12 (1) ◽  
Author(s):  
Jana Žiarovská ◽  
Lucia Zeleňáková ◽  
Miroslava Kačániová ◽  
Eloy Fernández Cusimamani
Keyword(s):  
Vitis Vinifera ◽  
Genomic Sequence ◽  
Vitis Vinifera L ◽  
Direct Pcr ◽  
Sequence Identification

scholarly journals Serological Reactivity in Citrus Tristeza Virus Strains in India

1993 ◽  
Vol 12 (12) ◽  
Author(s):  
N. K Chakraborty ◽  
Y. S. Ahlawat ◽  
A. Varma ◽  
K. J. Chandra ◽  
S. Ramapandu ◽  
...  
Keyword(s):  
Citrus Tristeza Virus ◽  
Serological Reactivity ◽  
Tristeza Virus ◽  
Virus Strains ◽  
Citrus Tristeza

scholarly journals Detection of Treponema pallidum (Syphilis) Antibodies among HIV-Infected Individuals and Sexually-active Attendees of Two Health Facilities in Port Harcourt, Nigeria

2020 ◽  
pp. 17-23
Author(s):  
Iheanyi O. Okonko ◽  
Tochi I. Cookey ◽  
Sofiat Adewuyi-Oseni ◽  
Amaka M. Awanye
Keyword(s):  
Treponema Pallidum ◽  
Rapid Test ◽  
Test Strip ◽  
Cross Sectional Study ◽  
Sexually Active ◽  
Cross Sectional ◽  
Port Harcourt ◽  
Serological Reactivity ◽  
Hiv Antibodies ◽  
Hiv 1

Aim: This study reports on the detection of Treponema pallidum (syphilis) antibodies in HIV infected patients in Port Harcourt, Nigeria. Screening for syphilis was carried out to determine the prevalence levels of these infections, as biological markers of risk, modes, and time functions of their transmission. Study Design: Cross-sectional study. Place and Duration of Study: University of Port Harcourt Teaching Hospital (UPTH) and O.B. Lulu Briggs Medical Centre, University of Port Harcourt, both in Port Harcourt, Nigeria, between August 2012 and July 2015. Methods: A total of 100 HIV-infected individuals and 100 sexually-active attendees were recruited for this study. Samples of blood were collected and re-screened for the presence of HIV antibodies using the Determine HIV-1/2 (Alere), HIV ½ Stat-Pak (Chembio), and HIV-1/2/P24/O ELISA kit (Dia.Pro). The same set of samples were screened for Treponema pallidum specific antibodies using the syphilis Ultra Rapid Test Strip (ACON(R), USA) and syphilis rapid strips (Global, USA) following the respective manufacturer's instructions. Results: Among the 200 samples, serological reactivity was detected for syphilis in 3(1.5%). The incidence of syphilis was higher in males (2.0%) than in females (1.0%). Age, sex, and locality did not significantly (P>0.05) influence the rate of syphilis. Conclusion: This study further confirms the presence of syphilis among the population studied. Routine screening of Syphilis among patients is therefore advocated.

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