Application of high-throughput, high-resolution and cost-effective next generation sequencing-based large-scale HLA typing in donor registry

2014 ◽  
Vol 85 (1) ◽  
pp. 20-28 ◽  
Author(s):  
M. Zhou ◽  
D. Gao ◽  
X. Chai ◽  
J. Liu ◽  
Z. Lan ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
High Resolution ◽  
High Throughput ◽  
Large Scale ◽  
Cost Effective ◽  
Hla Typing ◽  
Next Generation ◽  
Generation Sequencing

Next-Generation Sequencing: An Emerging Tool for Drug Designing

2019 ◽  
Vol 25 (31) ◽  
pp. 3350-3357 ◽  
Author(s):  
Pooja Tripathi ◽  
Jyotsna Singh ◽  
Jonathan A. Lal ◽  
Vijay Tripathi
Keyword(s):  
Next Generation Sequencing ◽  
High Throughput ◽  
Large Scale ◽  
Massively Parallel Sequencing ◽  
Genomic Research ◽  
Biological Research ◽  
Next Generation ◽  
Human Welfare ◽  
Drug Designing ◽  
Generation Sequencing

Background: With the outbreak of high throughput next-generation sequencing (NGS), the biological research of drug discovery has been directed towards the oncology and infectious disease therapeutic areas, with extensive use in biopharmaceutical development and vaccine production. Method: In this review, an effort was made to address the basic background of NGS technologies, potential applications of NGS in drug designing. Our purpose is also to provide a brief introduction of various Nextgeneration sequencing techniques. Discussions: The high-throughput methods execute Large-scale Unbiased Sequencing (LUS) which comprises of Massively Parallel Sequencing (MPS) or NGS technologies. The Next geneinvolved necessarily executes Largescale Unbiased Sequencing (LUS) which comprises of MPS or NGS technologies. These are related terms that describe a DNA sequencing technology which has revolutionized genomic research. Using NGS, an entire human genome can be sequenced within a single day. Conclusion: Analysis of NGS data unravels important clues in the quest for the treatment of various lifethreatening diseases and other related scientific problems related to human welfare.

scholarly journals Validation of variants using cost effective highresolution melting (HRM) analysis predicted from target re-sequencing in Eucalyptus

2020 ◽  
Vol 79 (2) ◽  
pp. 105-113
Author(s):  
Abdul Bari Muneera Parveen ◽  
Divya Lakshmanan ◽  
Modhumita Ghosh Dasgupta
Keyword(s):  
Next Generation Sequencing ◽  
Large Scale ◽  
Sequence Data ◽  
Cost Effective ◽  
Nucleotide Polymorphisms ◽  
Next Generation ◽  
Time Saving ◽  
Hrm Analysis ◽  
The Cost ◽  
Generation Sequencing

The advent of next-generation sequencing has facilitated large-scale discovery and mapping of genomic variants for high-throughput genotyping. Several research groups working in tree species are presently employing next generation sequencing (NGS) platforms for marker discovery, since it is a cost effective and time saving strategy. However, most trees lack a chromosome level genome map and validation of variants for downstream application becomes obligatory. The cost associated with identifying potential variants from the enormous amount of sequence data is a major limitation. In the present study, high resolution melting (HRM) analysis was optimized for rapid validation of single nucleotide polymorphisms (SNPs), insertions or deletions (InDels) and simple sequence repeats (SSRs) predicted from exome sequencing of parents and hybrids of Eucalyptus tereticornis Sm. ? Eucalyptus grandis Hill ex Maiden generated from controlled hybridization. The cost per data point was less than 0.5 USD, providing great flexibility in terms of cost and sensitivity, when compared to other validation methods. The sensitivity of this technology in variant detection can be extended to other applications including Bar-HRM for species authentication and TILLING for detection of mutants.

scholarly journals Next-Generation Sequencing Technologies in Blood Group Typing

2019 ◽  
Vol 47 (1) ◽  
pp. 4-13 ◽  
Author(s):  
Daniel Fürst ◽  
Chrysanthi Tsamadou ◽  
Christine Neuchel ◽  
Hubert Schrezenmeier ◽  
Joannis Mytilineos ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Blood Group ◽  
Large Scale ◽  
Cost Effective ◽  
Molecular Testing ◽  
Blood Group Antigens ◽  
Next Generation ◽  
Sequencing Technologies ◽  
Blood Group Typing ◽  
Generation Sequencing

Sequencing of the human genome has led to the definition of the genes for most of the relevant blood group systems, and the polymorphisms responsible for most of the clinically relevant blood group antigens are characterized. Molecular blood group typing is used in situations where erythrocytes are not available or where serological testing was inconclusive or not possible due to the lack of antisera. Also, molecular testing may be more cost-effective in certain situations. Molecular typing approaches are mostly based on either PCR with specific primers, DNA hybridization, or DNA sequencing. Particularly the transition of sequencing techniques from Sanger-based sequencing to next-generation sequencing (NGS) technologies has led to exciting new possibilities in blood group genotyping. We describe briefly the currently available NGS platforms and their specifications, depict the genetic background of blood group polymorphisms, and discuss applications for NGS approaches in immunohematology. As an example, we delineate a protocol for large-scale donor blood group screening established and in use at our institution. Furthermore, we discuss technical challenges and limitations as well as the prospect for future developments, including long-read sequencing technologies.

scholarly journals High-resolution, high-throughput HLA genotyping by next-generation sequencing

2009 ◽  
Vol 74 (5) ◽  
pp. 393-403 ◽  
Author(s):  
G. Bentley ◽  
R. Higuchi ◽  
B. Hoglund ◽  
D. Goodridge ◽  
D. Sayer ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
High Resolution ◽  
High Throughput ◽  
Next Generation ◽  
Hla Genotyping ◽  
Generation Sequencing

Application of high‐throughput next‐generation sequencing for HLA typing of DNA extracted from postprocessing cord blood units

HLA ◽  
2019 ◽  
Vol 94 (2) ◽  
pp. 141-146 ◽  
Author(s):  
Vani Seshasubramanian ◽  
Meganathan Venugopal ◽  
Aruna D. S. Kannan ◽  
Chandramouleeswaran Naganathan ◽  
Nirmal K. Manisekar ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Cord Blood ◽  
High Throughput ◽  
Hla Typing ◽  
Next Generation ◽  
Generation Sequencing

scholarly journals High-Resolution HLA Typing of HLA-A, -B, -C, -DRB1, and -DQB1 in Kinh Vietnamese by Using Next-Generation Sequencing

2020 ◽  
Vol 11 ◽  
Author(s):  
Minh Duc Do ◽  
Linh Gia Hoang Le ◽  
Vinh The Nguyen ◽  
Tran Ngoc Dang ◽  
Nghia Hoai Nguyen ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
High Resolution ◽  
Hla Typing ◽  
Next Generation ◽  
Generation Sequencing
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