Examination of wound healing after curettage by multiphoton tomography of human skin in vivo

2017 ◽  
Vol 23 (4) ◽  
pp. 452-458 ◽  
Author(s):  
S. Springer ◽  
M. Zieger ◽  
A. Böttcher ◽  
J. Lademann ◽  
M. Kaatz
Keyword(s):  
Wound Healing ◽  
Human Skin ◽  
Multiphoton Tomography

scholarly journals Root extractive from Daphne genkwa benefits in wound healing of anal fistula through up-regulation of collagen genes in human skin fibroblasts

2017 ◽  
Vol 37 (2) ◽  
Author(s):  
Dong Yang ◽  
Jun-hua Xu ◽  
Ren-jie Shi
Keyword(s):  
Wound Healing ◽  
Human Skin ◽  
Anal Fistula ◽  
Healing Time ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Protein Levels ◽  
Daphne Genkwa

Wound healing is the main problem in the therapy of anal fistula (AF). Daphne genkwa root has been traditionally used as an agent to soak sutures in operation of AF patients, but its function in wound healing remains largely unclear. The aim of the present study was to illuminate mechanisms of D. genkwa root treatment on AF. In the present study, 60 AF patients after surgery were randomly divided into two groups, external applied with or without the D. genkwa extractive. Wound healing times were compared and granulation tissues were collected. In vitro, we constructed damaged human skin fibroblasts (HSFs) with the treatment of TNF-α (10 μg/ml). Cell Count Kit-8 (CCK-8) and flow cytometry analysis were used to determine the effects of D. genkwa root extractive on cell viability, cell cycle and apoptosis of damaged HSFs. Furthermore, protein levels of TGF-β, COL1A1, COL3A1, Timp-1, matrix metalloproteinase (MMP)-3 (MMP-3) and MEK/ERK signalling pathways were investigated both in vivo and in vitro. Results showed that D. genkwa root extractive greatly shortens the wound healing time in AF patients. In granulation tissues and HSFs, treatment with the extractive significantly elevated the expressions of COL1A1, COL3A1, Timp-1, c-fos and Cyclin D1, while reduced the expression of MMP-3. Further detection presented that MEK/ERK signalling was activated after the stimulation of extractive in HSFs. Our study demonstrated that extractive from D. genkwa root could effectively improve wound healing in patients with AF via the up-regulation of fibroblast proliferation and expressions of COL1A1 and COL3A1.

In vivo multiphoton tomography: a non invasive powerful tool for biochemical investigation of human skin

2007 ◽  
Author(s):  
Ronan Le Harzic ◽  
Anne Colonna ◽  
Rainer Bückle ◽  
Alexander Ehlers ◽  
Christophe Hadjur ◽  
...  
Keyword(s):  
Human Skin ◽  
Non Invasive ◽  
Biochemical Investigation ◽  
Multiphoton Tomography

In vivo non-invasive multiphoton tomography of human skin

2005 ◽  
Author(s):  
Karsten König ◽  
Iris Riemann ◽  
Alexander Ehlers ◽  
Ronan Le Harzic
Keyword(s):  
Human Skin ◽  
Non Invasive ◽  
Multiphoton Tomography

Intravital multiphoton tomography as an appropriate tool for non-invasive in vivo analysis of human skin affected with atopic dermatitis

2011 ◽  
Author(s):  
Volker Huck ◽  
Christian Gorzelanny ◽  
Kai Thomas ◽  
Christian Mess ◽  
Valentina Dimitrova ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Human Skin ◽  
Non Invasive ◽  
In Vivo Analysis ◽  
Multiphoton Tomography

3D-printed chitosan-based scaffolds: An in vitro study of human skin cell growth and an in-vivo wound healing evaluation in experimental diabetes in rats

2018 ◽  
Vol 199 ◽  
pp. 593-602 ◽  
Author(s):  
Claudio Intini ◽  
Lisa Elviri ◽  
Jaydee Cabral ◽  
Sonya Mros ◽  
Carlo Bergonzi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Growth ◽  
Human Skin ◽  
Experimental Diabetes ◽  
In Vitro Study ◽  
Vitro Study ◽  
Skin Cell ◽  
3D Printed

In vivo multiphoton tomography of skin during wound healing and scar formation

2007 ◽  
Author(s):  
Iris Riemann ◽  
Alexander Ehlers ◽  
Ronan LeHarzic ◽  
Sven Martin ◽  
Annette Reif ◽  
...  
Keyword(s):  
Wound Healing ◽  
Scar Formation ◽  
Multiphoton Tomography

scholarly journals Accelerated complete human skin architecture restoration after wounding by nanogenerator-driven electrostimulation

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Aiping Liu ◽  
Yin Long ◽  
Jun Li ◽  
Long Gu ◽  
Aos Karim ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Skin ◽  
Wound Closure ◽  
Normal Skin ◽  
Xenograft Model ◽  
Clinical Use ◽  
Inflammatory Phase ◽  
Complete Restoration ◽  
Skin Xenograft

Abstract Background Electrostimulation (ES) therapy for wound healing is limited in clinical use due to barriers such as cumbersome equipment and intermittent delivery of therapy. Methods We adapted a human skin xenograft model that can be used to directly examine the nanogenerator-driven ES (NG-ES) effects on human skin in vivo—an essential translational step toward clinical application of the NG-ES technique for wound healing. Results We show that NG-ES leads to rapid wound closure with complete restoration of normal skin architecture within 7 days compared to more than 30 days in the literature. NG-ES accelerates the inflammatory phase of wound healing with more rapid resolution of neutrophils and macrophages and enhances wound bed perfusion with more robust neovascularization. Conclusion Our results support the translational evaluation and optimization of the NG-ES technology to deliver convenient, efficient wound healing therapy for use in human wounds. Graphic abstract

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