Ultraviolet radiation and nanoparticle induced intracellular free radicals generation measured in human keratinocytes by electron paramagnetic resonance spectroscopy

2013 ◽  
Vol 20 (2) ◽  
pp. 182-193 ◽  
Author(s):  
F. Rancan ◽  
B. Nazemi ◽  
S. Rautenberg ◽  
M. Ryll ◽  
S. Hadam ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Free Radicals ◽  
Ultraviolet Radiation ◽  
Electron Paramagnetic Resonance Spectroscopy ◽  
Human Keratinocytes ◽  
Resonance Spectroscopy ◽  
Paramagnetic Resonance ◽  
Electron Paramagnetic

Time-resolved electron paramagnetic resonance spectroscopy of organic free radicals anchored to silica surfaces

1991 ◽  
Vol 95 (26) ◽  
pp. 10547-10549 ◽  
Author(s):  
Malcolm D. E. Forbes ◽  
Katerina E. Dukes ◽  
Tanya L. Myers ◽  
Heather D. Maynard ◽  
Christopher S. Breivogel ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Free Radicals ◽  
Electron Paramagnetic Resonance Spectroscopy ◽  
Resonance Spectroscopy ◽  
Paramagnetic Resonance ◽  
Time Resolved ◽  
Silica Surfaces ◽  
Electron Paramagnetic

scholarly journals Application of Electron Paramagnetic Resonance Spectroscopy to Comparative Examination of Different Groups of Free Radicals in Thermal Injuries Treated with Propolis and Silver Sulphadiazine

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Pawel Olczyk ◽  
Pawel Ramos ◽  
Marcin Bernas ◽  
Katarzyna Komosinska-Vassev ◽  
Jerzy Stojko ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Free Radicals ◽  
Electron Paramagnetic Resonance Spectroscopy ◽  
Numerical Procedure ◽  
Resonance Spectroscopy ◽  
Paramagnetic Resonance ◽  
Tissue Samples ◽  
Thermal Injuries ◽  
Electron Paramagnetic ◽  
Silver Sulphadiazine

Different groups of free radicals expressed in burn wounds treated with propolis and silver sulphadiazine were examined. The thermal effect forms major types of free radicals in a wound because of the breaking of chemical bonds. Free radicals, located in the heated skin, were tested after 21 days of treating by these two substances. The aim of this work was to find the method for determination of types and concentrations of different groups of free radicals in wound after high temperature impact during burning. The effects of the therapy by propolis and silver sulphadiazine on free radicals were studied. Since the chemical methods of free radicals studies are destructive, the usefulness of the electron paramagnetic resonance spectroscopy was tested in this work. The electron paramagnetic resonance spectra measured with the microwave power of 2.2 mW were numerically fitted by theoretical curves of Gaussian and Lorentzian shapes. The experimental electron paramagnetic resonance spectra of tissue samples are best fitted by the sum of one Gauss and two Lorentz lines. An innovatory numerical procedure of spectroscopic skin analysis was presented. It is very useful in the alternative medicine studies.

scholarly journals Effect of UV Irradiation on Interactions ofα-Lipoic Acid with Free Radicals

2013 ◽  
Vol 2013 ◽  
pp. 1-5
Author(s):  
Paweł Ramos ◽  
Piotr Pepliński ◽  
Barbara Pilawa
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Free Radicals ◽  
Uv Irradiation ◽  
Lipoic Acid ◽  
Electron Paramagnetic Resonance Spectroscopy ◽  
Antioxidant Properties ◽  
Resonance Spectroscopy ◽  
Paramagnetic Resonance ◽  
Pharmacological Substance ◽  
Electron Paramagnetic

Changes of antioxidant properties ofα-lipoic acid (LA) after UV irradiation were studied. LA is the typical drug used in diabetic neuropathy. Quenching of free radicals is an important factor of therapy by using this substance.α-Lipoic acid is exposed to UV irradiation during the storage. The aim of our studies was to examine the effect of UV irradiation on the interactions of LA with free radicals. Theα-lipoic acid was irradiated by UVA 315–400 nm light during 10 to 110 minutes by intervals of 10 minutes. The electron paramagnetic resonance spectroscopy was used as the experimental technique. The antioxidant properties of LA were spectroscopically confirmed. The strong effect of UV irradiation on interactions ofα-lipoic acid with free radicals was observed. It was pointed out that interactions of LA with free radicals decrease after its exposition on UV. The interactions of LA with free radicals were higher after the sample irradiation during 10 minutes than for the samples irradiated longer (20–110 minutes). The results are important for problems connected with photomedicine; they pointed out thatα-lipoic acid should not be stored on UV exposition. Application of electron paramagnetic resonance spectroscopy to characterize interactions of pharmacological substance with free radicals was confirmed.

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