scholarly journals From Conflict to Bridges: Towards Constructive Use of Conflict Frames in the Control of Bovine Tuberculosis

2019 ◽  
Vol 60 (2) ◽  
pp. 482-504 ◽  
Author(s):  
Caroline Keenan ◽  
Clare Saunders ◽  
Stephan Price ◽  
Stephen Hinchliffe ◽  
Robbie A. McDonald
Keyword(s):  
Bovine Tuberculosis ◽  
Conflict Frames

Bovine tuberculosis: a zoonotic target for medicinal plants

Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
LJ McGaw ◽  
N Lall ◽  
T Hlokwe ◽  
A Michel ◽  
JJM Meyer ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Bovine Tuberculosis

Assessment of the Use of PCR as an Early Diagnostic Indicator of Bovine Tuberculosis in Dairy Farms

2019 ◽  
Vol 09 (01) ◽  
Author(s):  
Antonia Mataragka ◽  
Virginia Fyntani ◽  
Kyriaki Sotirakoglou ◽  
Aristomenis Katsiolis ◽  
Chrysoula Dile ◽  
...  
Keyword(s):  
Bovine Tuberculosis ◽  
Dairy Farms ◽  
Diagnostic Indicator ◽  
Early Diagnostic

Purification in antigen and application in diagnosis of IFN-γ-ELISA detection of bovine tuberculosis

2011 ◽  
Vol 37 (1) ◽  
pp. 82-85
Author(s):  
Mei-zhen QIU ◽  
Wang-ping ZHOU ◽  
Bing-nan XIAO ◽  
Li-fei DU ◽  
Shu-guang HU ◽  
...  
Keyword(s):  
Bovine Tuberculosis ◽  
Ifn Γ

scholarly journals A case of early neonate bovine tuberculosis in Ethiopia

2020 ◽  
Author(s):  
Getnet Abie Mekonnen ◽  
Balako Gumi ◽  
Stefan Berg ◽  
Andrew J. K. Conlan ◽  
Gobena Ameni ◽  
...  
Keyword(s):  
Bovine Tuberculosis

A simulation model for the spread of bovine tuberculosis within New Zealand cattle herds

1997 ◽  
Vol 32 (1-2) ◽  
pp. 57-75 ◽  
Author(s):  
N.D. Barlow ◽  
J.M. Kean ◽  
G. Hickling ◽  
P.G. Livingstone ◽  
A.B. Robson
Keyword(s):  
New Zealand ◽  
Simulation Model ◽  
Bovine Tuberculosis ◽  
Cattle Herds

scholarly journals A molecularly defined skin test reagent for the diagnosis of bovine tuberculosis compatible with vaccination against Johne’s Disease

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sonya Middleton ◽  
Sabine Steinbach ◽  
Michael Coad ◽  
Kevina McGill ◽  
Colm Brady ◽  
...  
Keyword(s):  
Skin Test ◽  
Recombinant Proteins ◽  
Bovine Tuberculosis ◽  
Synthetic Peptides ◽  
Blood Test ◽  
High Specificity ◽  
Active Components ◽  
Interferon Gamma Release Assays ◽  
Skin Responses

AbstractTuberculin Purified Protein Derivatives (PPDs) exhibit multiple limitations: they are crude extracts from mycobacterial cultures with largely unknown active components; their production depends on culture of mycobacteria requiring expensive BCL3 production facilities; and their potency depends on the technically demanding guinea pig assay. To overcome these limitations, we developed a molecularly defined tuberculin (MDT) by adding further antigens to our prototype reagent composed of ESAT-6, CFP-10 and Rv3615c (DIVA skin test, DST). In vitro screening using PBMC from infected and uninfected cattle shortlisted four antigens from a literature-based list of 18 to formulate the MDT. These four antigens plus the previously identified Rv3020c protein, produced as recombinant proteins or overlapping synthetic peptides, were formulated together with the three DST antigens into the MDT to test cattle experimentally and naturally infected with M. bovis, uninfected cattle and MAP vaccinated calves. We demonstrated significant increases in MDT-induced skin responses compared to DST in infected animals, whilst maintaining high specificity in unvaccinated or MAP vaccinated calves. Further, MDT can also be applied in in vitro blood-based interferon-gamma release assays. Thus, MDT promises to be a robust diagnostic skin and blood test reagent overcoming some of the limitations of PPDs and warrants full validation.

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