scholarly journals Nonparametric detection of changes over time in image data from fluorescence microscopy of living cells

2021 ◽  
Author(s):  
Kathrin Bissantz ◽  
Nicolai Bissantz ◽  
Katharina Proksch
Keyword(s):  
Fluorescence Microscopy ◽  
Image Data ◽  
Living Cells ◽  
Nonparametric Detection ◽  
Changes Over Time ◽  
Over Time

scholarly journals CLASSIFICATION UNDER LABEL NOISE BASED ON OUTDATED MAPS

2017 ◽  
Vol IV-1/W1 ◽  
pp. 215-222 ◽  
Author(s):  
A. Maas ◽  
F. Rottensteiner ◽  
C. Heipke
Keyword(s):  
Classical Method ◽  
Image Data ◽  
Training Data ◽  
Label Noise ◽  
Training Technique ◽  
Class Labels ◽  
Noise Tolerant ◽  
Changes Over Time ◽  
Over Time

Supervised classification of remotely sensed images is a classical method for change detection. The task requires training data in the form of image data with known class labels, whose manually generation is time-consuming. If the labels are acquired from the outdated map, the classifier must cope with errors in the training data. These errors, referred to as label noise, typically occur in clusters in object space, because they are caused by land cover changes over time. In this paper we adapt a label noise tolerant training technique for classification, so that the fact that changes affect larger clusters of pixels is considered. We also integrate the existing map into an iterative classification procedure to act as a prior in regions which are likely to contain changes. Our experiments are based on three test areas, using real images with simulated existing databases. Our results show that this method helps to distinguish between real changes over time and false detections caused by misclassification and thus improves the accuracy of the classification results.

Application of FRAP and digitized fluorescence microscopy to problems in cell membrane dynamics

1988 ◽  
Vol 46 ◽  
pp. 118-119
Author(s):  
K. Jacobson ◽  
A. Ishihara ◽  
B. Holifield ◽  
F. Zhang
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Biology ◽  
Extended Period ◽  
Dynamic Structure ◽  
Living Cells ◽  
Membrane Dynamics ◽  
Molecular Cell Biology ◽  
Image Averaging ◽  
Single Living Cells ◽  
Single Living

Our laboratory is concerned with understanding the dynamic structure of the plasma membrane with particular reference to the movement of membrane constituents during cell locomotion. In addition to the standard tools of molecular cell biology, we employ both fluorescence recovery after photo- bleaching (FRAP) and digitized fluorescence microscopy (DFM) to investigate individual cells. FRAP allows the measurement of translational mobility of membrane and cytoplasmic molecules in small regions of single, living cells. DFM is really a new form of light microscopy in that the distribution of individual classes of ions, molecules, and macromolecules can be followed in single, living cells. By employing fluorescent antibodies to defined antigens or fluorescent analogs of cellular constituents as well as ultrasensitive, electronic image detectors and video image averaging to improve signal to noise, fluorescent images of living cells can be acquired over an extended period without significant fading and loss of cell viability.

Comparison of two devices for measuring exercise transcutaneous oxygen pressures in patients with claudication

VASA ◽  
2015 ◽  
Vol 44 (5) ◽  
pp. 355-362 ◽  
Author(s):  
Marie Urban ◽  
Alban Fouasson-Chailloux ◽  
Isabelle Signolet ◽  
Christophe Colas Ribas ◽  
Mathieu Feuilloy ◽  
...  
Keyword(s):  
Oxygen Pressure ◽  
Optical Sensors ◽  
Chemical Sensors ◽  
Transcutaneous Oxygen ◽  
Starting Values ◽  
Two Systems ◽  
Transcutaneous Oxygen Pressure ◽  
New Sensors ◽  
Changes Over Time ◽  
Over Time

Abstract. Summary: Background: We aimed at estimating the agreement between the Medicap® (photo-optical) and Radiometer® (electro-chemical) sensors during exercise transcutaneous oxygen pressure (tcpO2) tests. Our hypothesis was that although absolute starting values (tcpO2rest: mean over 2 minutes) might be different, tcpO2-changes over time and the minimal value of the decrease from rest of oxygen pressure (DROPmin) results at exercise shall be concordant between the two systems. Patients and methods: Forty seven patients with arterial claudication (65 + / - 7 years) performed a treadmill test with 5 probes each of the electro-chemical and photo-optical devices simultaneously, one of each system on the chest, on each buttock and on each calf. Results: Seventeen Medicap® probes disconnected during the tests. tcpO2rest and DROPmin values were higher with Medicap® than with Radiometer®, by 13.7 + / - 17.1 mm Hg and 3.4 + / - 11.7 mm Hg, respectively. Despite the differences in absolute starting values, changes over time were similar between the two systems. The concordance between the two systems was approximately 70 % for classification of test results from DROPmin. Conclusions: Photo-optical sensors are promising alternatives to electro-chemical sensors for exercise oximetry, provided that miniaturisation and weight reduction of the new sensors are possible.

Neuropsychological Symptoms in Posttraumatic Stress Disorder and Changes Over Time

2007 ◽  
Author(s):  
Miranda Olff ◽  
Mirjam Nijdam ◽  
Kristin Samuelson ◽  
Julia Golier ◽  
Mariel Meewisse ◽  
...  
Keyword(s):  
Posttraumatic Stress Disorder ◽  
Posttraumatic Stress ◽  
Stress Disorder ◽  
Neuropsychological Symptoms ◽  
Changes Over Time ◽  
Over Time

Changes Over Time in Adaptive Behavior Functioning of Twice-Exceptional Children

2010 ◽  
Author(s):  
Rebecca D. Stinson ◽  
Zachary Sussman ◽  
Megan Foley Nicpon ◽  
Allison L. Allmon ◽  
Courtney Cornick ◽  
...  
Keyword(s):  
Adaptive Behavior ◽  
Exceptional Children ◽  
Twice Exceptional ◽  
Changes Over Time ◽  
Over Time

Kategorisierung der Schädelform des Cavalier King Charles Spaniels

2019 ◽  
Vol 47 (02) ◽  
pp. 133-133
Keyword(s):  
Pilot Study ◽  
Cavalier King Charles Spaniel ◽  
Conformation Changes ◽  
Veterinary Record ◽  
Changes Over Time ◽  
Over Time

Knowler SP, Gillstedt L, Mitchell TJ et al. Pilot study of head conformation changes over time in the Cavalier King Charles spaniel breed. Veterinary Record 2019. doi:10.1136/vr.105135.

Uneven Decoupling

2019 ◽  
Vol 5 (4) ◽  
pp. 410-427 ◽  
Author(s):  
Ryan P. Thombs ◽  
Xiaorui Huang
Keyword(s):  
Economic Growth ◽  
Co2 Emissions ◽  
Ecological Modernization ◽  
Climate Mitigation ◽  
Data Limitations ◽  
Treadmill Of Production ◽  
Global North ◽  
Organization Of Production ◽  
Changes Over Time ◽  
Over Time

The macro-comparative decoupling literature has often sought to test the arguments made by the treadmill of production (TP) and ecological modernization (EM) theories. However, due to data limitations, these studies have been limited to analyzing the years after 1960. Given that both theories discuss historical processes operating before 1960, analyzing pre-1960 data is warranted to more comprehensively test the propositions made by both theories. We assess the long-term relationship between economic growth and CO2 emissions from 1870 to 2014 using a sample of global North nations. We use Prais-Winsten regression models with time interactions to assess whether, when, and how much CO2 emissions have decoupled from economic growth over time. We find that significant relative decoupling has occurred twice since 1870: during the last 30 years of the nineteenth century, the timing of which is contrary to what both the EM and TP theories might expect, and after 1970. We also observe that the relationship remained relatively stable from the turn of the twentieth century to approximately 1970, which aligns with the arguments made by the classical TP work. We conclude that shifts in the global organization of production have shaped the magnitude of the economic growth–CO2 emissions relationship and its changes over time, which has implications for climate mitigation policy.

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