Metabolic adaptations of human alveolar macrophages upon activation by lipopolysaccharide in vivo

2021 ◽  
Author(s):  
Natasja A. Otto ◽  
Joe M. Butler ◽  
Ivan Ramirez‐Moral ◽  
Jacobien J. Hoogerwerf ◽  
Riekelt H. Houtkooper ◽  
...  
Keyword(s):  
Alveolar Macrophages ◽  
Metabolic Adaptations ◽  
Human Alveolar Macrophages

scholarly journals Tissue factor and factor VII messenger RNAs in human alveolar macrophages: effects of breathing ozone

Blood ◽  
1990 ◽  
Vol 75 (1) ◽  
pp. 122-127 ◽  
Author(s):  
MP McGee ◽  
R Devlin ◽  
G Saluta ◽  
H Koren
Keyword(s):  
Alveolar Macrophage ◽  
Tissue Factor ◽  
Alveolar Macrophages ◽  
Factor Vii ◽  
Fibrinopeptide A ◽  
Factor Activity ◽  
Tissue Factor Activity ◽  
Mrna Concentration ◽  
Human Alveolar Macrophages

Abstract This study was performed to determine if genes for tissue factor and factor VII proteins are expressed and regulated in vivo in lung macrophages during inflammation. Human alveolar macrophages and alveolar fluids were obtained 18 hours after healthy male adults were exposed, for 2 hours during intermittent exercise, to either air or air with 0.4 ppm ozone, added as a model toxic respiratory agent. Messenger RNA (mRNA) for both tissue factor and factor VII were demonstrated in macrophages isolated after subjects were exposed to unpolluted control air. With the same subjects examined after breathing ozone, the following changes were observed: tissue factor mRNA concentration in macrophages increased 2.6 +/- 0.47-fold. Factor VII mRNA concentration was reduced 0.64 +/- 0.24-fold. Total numbers of macrophages recovered did not change significantly. Ratios of nuclear:cytoplasmic areas of cytocentrifuged macrophages were augmented by 24.8% +/- 3%, giving morphometric evidence that immature cell forms increased in the population. In the lavage, tissue factor activity was increased 2.1 +/- 0.3-fold, while amounts of lipid phosphorous, which estimate total membrane lipids, and estimated volumes of alveolar fluid were not significantly changed. Factor VII activity and fibrinopeptide A levels in lavage were increased approximately twofold. These results using rapidly isolated, noncultured cells indicate that tissue factor and factor VII mRNA are synthesized in the alveolar macrophage population in vivo. In addition, evidence was found that as a result of breathing ozone, a shift in alveolar macrophage maturity occurred in association with tissue factor mRNA, tissue factor activity, and factor VII activity increases, and with formation of fibrinopeptide A in alveolar fluids.

Production of Chemotactic Factor(s) by in vivo Cultured Human Alveolar Macrophages

CHEST Journal ◽  
1979 ◽  
Vol 75 (2) ◽  
pp. 224
Author(s):  
William W. Merrill ◽  
Gary P. Naegel ◽  
Richard A. Matthay ◽  
Herbert Y. Reynolds
Keyword(s):  
Alveolar Macrophages ◽  
Chemotactic Factor ◽  
Human Alveolar Macrophages

Production of Chemotactic Factor(s) by in vivo Cultured Human Alveolar Macrophages

CHEST Journal ◽  
1979 ◽  
Vol 75 (2) ◽  
pp. 224 ◽  
Author(s):  
William W. Merrill ◽  
Gary P. Naegel ◽  
Richard A. Matthay ◽  
Herbert Y. Reynolds
Keyword(s):  
Alveolar Macrophages ◽  
Chemotactic Factor ◽  
Human Alveolar Macrophages

scholarly journals Phenotypic comparison of human alveolar macrophages before and after in vivo rhinovirus 16 challenge

2021 ◽  
Author(s):  
Linsey E. S. Groot ◽  
Paul Brinkman ◽  
Yanaika S. Sabogal Piñeros ◽  
Josef Priller ◽  
Jörg Hamann ◽  
...  
Keyword(s):  
Alveolar Macrophages ◽  
Human Alveolar Macrophages ◽  
Before And After

Human alveolar macrophages: effects of endotoxin in vitro

1980 ◽  
Vol 30 (3) ◽  
pp. 753-758
Author(s):  
W B Davis ◽  
I S Barsoum ◽  
P W Ramwell ◽  
H Yeager
Keyword(s):  
Alveolar Macrophages ◽  
Fiberoptic Bronchoscopy ◽  
Significant Inhibition ◽  
Mononuclear Phagocytes ◽  
Human Alveolar Macrophages ◽  
In Vitro Effects ◽  
Gram Negative Organisms ◽  
And Function

Experiments were performed to evaluate the in vitro effects of Escherichia coli lipopolysaccharide on viability and function of human alveolar macrophages. Alveolar macrophages were obtained by fiberoptic bronchoscopy and saline bronchial lavage from 12 normal, nonsmoking volunteers. Cells were incubated with different concentrations of E. coli endotoxin for 1 and 24 h. Endotoxin (10 microgram/ml and more) was cytotoxic for alveolar macrophages after 24 h of incubation and induced significant inhibition of phagocytosis, adherence, and spreading. The effects of endotoxin on alveolar macrophage viability and function were dose and time dependent and were not influenced by indomethacin. Thus, human alveolar macrophages, like other mononuclear phagocytes, are extremely sensitive to endotoxin effects; these observations may be relevant in conditions in which endotoxin may be in contact with alveolar macrophages in vivo: endobronchial infections with gram-negative organisms, byssinosis, chronic bronchitis of grain handles, and humidifier fever.

scholarly journals CD116+ fetal precursors migrate to the perinatal lung and give rise to human alveolar macrophages

2022 ◽  
Vol 219 (2) ◽  
Author(s):  
Elza Evren ◽  
Emma Ringqvist ◽  
Jean-Marc Doisne ◽  
Anna Thaller ◽  
Natalie Sleiers ◽  
...  
Keyword(s):  
Alveolar Macrophage ◽  
Alveolar Macrophages ◽  
Fetal Liver ◽  
Fetal Lung ◽  
Gene Signature ◽  
Human Macrophage ◽  
Humanized Mouse Model ◽  
Human Alveolar Macrophages ◽  
Macrophage Precursors

Despite their importance in lung health and disease, it remains unknown how human alveolar macrophages develop early in life. Here we define the ontogeny of human alveolar macrophages from embryonic progenitors in vivo, using a humanized mouse model expressing human cytokines (MISTRG mice). We identified alveolar macrophage progenitors in human fetal liver that expressed the GM-CSF receptor CD116 and the transcription factor MYB. Transplantation experiments in MISTRG mice established a precursor–product relationship between CD34−CD116+ fetal liver cells and human alveolar macrophages in vivo. Moreover, we discovered circulating CD116+CD64−CD115+ macrophage precursors that migrated from the liver to the lung. Similar precursors were present in human fetal lung and expressed the chemokine receptor CX3CR1. Fetal CD116+CD64− macrophage precursors had a proliferative gene signature, outcompeted adult precursors in occupying the perinatal alveolar niche, and developed into functional alveolar macrophages. The discovery of the fetal alveolar macrophage progenitor advances our understanding of human macrophage origin and ontogeny.

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