scholarly journals BLIMP-1 is insufficient to induce antibody secretion in the absence of IRF4 in DT40 cells

2018 ◽  
Vol 87 (3) ◽  
pp. e12646 ◽  
Author(s):  
P. M. Budzyńska ◽  
M. K. Kyläniemi ◽  
O. Lassila ◽  
K.-P. Nera ◽  
J. Alinikula
Keyword(s):  
Dt40 Cells ◽  
Antibody Secretion ◽  
Induce Antibody

scholarly journals PRDX1 is essential for the viability and maintenance of reactive oxygen species in chicken DT40

2021 ◽  
Vol 43 (1) ◽  
Author(s):  
Takahito Moriwaki ◽  
Akari Yoshimura ◽  
Yuki Tamari ◽  
Hiroyuki Sasanuma ◽  
Shunichi Takeda ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Death ◽  
Intracellular Signaling ◽  
Reactive Oxygen ◽  
Fine Tuning ◽  
Oxygen Species ◽  
Ros Homeostasis ◽  
Intracellular Ros ◽  
Chromosomal Breaks ◽  
Dt40 Cells

Abstract Background Peroxiredoxin 1 (PRDX1) is a member of a ubiquitous family of thiol peroxidases that catalyze the reduction of peroxides, including hydrogen peroxide. It functions as an antioxidant enzyme, similar to catalase and glutathione peroxidase. PRDX1 was recently shown act as a sensor of reactive oxygen species (ROS) and play a role in ROS-dependent intracellular signaling pathways. To investigate its physiological functions, PRDX1 was conditionally disrupted in chicken DT40 cells in the present study. Results The depletion of PRDX1 resulted in cell death with increased levels of intracellular ROS. PRDX1-depleted cells did not show the accumulation of chromosomal breaks or sister chromatid exchange (SCE). These results suggest that cell death in PRDX1-depleted cells was not due to DNA damage. 2-Mercaptoethanol protected against cell death in PRDX1-depleted cells and also suppressed elevations in ROS. Conclusions PRDX1 is essential in chicken DT40 cells and plays an important role in maintaining intracellular ROS homeostasis (or in the fine-tuning of cellular ROS levels). Cells deficient in PRDX1 may be used as an endogenously deregulated ROS model to elucidate the physiological roles of ROS in maintaining proper cell growth.

scholarly journals The Constitutive Centromere Component CENP-50 Is Required for Recovery from Spindle Damage

2005 ◽  
Vol 25 (23) ◽  
pp. 10315-10328 ◽  
Author(s):  
Yukinori Minoshima ◽  
Tetsuya Hori ◽  
Masahiro Okada ◽  
Hiroshi Kimura ◽  
Tokuko Haraguchi ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Growth Rate ◽  
Mitotic Checkpoint ◽  
Loss Of Function ◽  
Wild Type ◽  
Centromere Function ◽  
Dt40 Cells ◽  
Precise Role ◽  
Chicken Dt40 Cell

ABSTRACT We identified CENP-50 as a novel kinetochore component. We found that CENP-50 is a constitutive component of the centromere that colocalizes with CENP-A and CENP-H throughout the cell cycle in vertebrate cells. To determine the precise role of CENP-50, we examined its role in centromere function by generating a loss-of-function mutant in the chicken DT40 cell line. The CENP-50 knockout was not lethal; however, the growth rate of cells with this mutation was slower than that of wild-type cells. We observed that the time for CENP-50-deficient cells to complete mitosis was longer than that for wild-type cells. Centromeric localization of CENP-50 was abolished in both CENP-H- and CENP-I-deficient cells. Coimmunoprecipitation experiments revealed that CENP-50 interacted with the CENP-H/CENP-I complex in chicken DT40 cells. We also observed severe mitotic defects in CENP-50-deficient cells with apparent premature sister chromatid separation when the mitotic checkpoint was activated, indicating that CENP-50 is required for recovery from spindle damage.

scholarly journals In vitro culture of hybridoma cells in agarose beads producing antibody secretion for two weeks

1992 ◽  
Vol 39 (1) ◽  
pp. 108-112 ◽  
Author(s):  
Ch. Cadic ◽  
B. Dupuy ◽  
I. Pianet ◽  
M. Merle ◽  
Ch. Margerin ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Hybridoma Cells ◽  
Agarose Beads ◽  
Antibody Secretion

Metabolism of hybridoma cells and antibody secretion at high cell densities in dialysis tubing

1991 ◽  
Vol 13 (11) ◽  
pp. 873-881 ◽  
Author(s):  
Claudia Käsehagen ◽  
Fritjof Linz ◽  
Gerlinde Kretzmer ◽  
Thomas Scheper ◽  
Karl Schügerl
Keyword(s):  
Hybridoma Cells ◽  
High Cell ◽  
Dialysis Tubing ◽  
Cell Densities ◽  
Antibody Secretion

Specific IgG antibody secretion from HLA tetramer isolated B cells

2005 ◽  
Vol 66 (8) ◽  
pp. 2
Author(s):  
Dessislava Kopchaliiska ◽  
Mary S. Leffell ◽  
Andrea A. Zachary
Keyword(s):  
B Cells ◽  
Igg Antibody ◽  
Specific Igg ◽  
Antibody Secretion

scholarly journals Mzb1 Protein Regulates Calcium Homeostasis, Antibody Secretion, and Integrin Activation in Innate-like B Cells

Immunity ◽  
2010 ◽  
Vol 33 (5) ◽  
pp. 723-735 ◽  
Author(s):  
Henrik Flach ◽  
Marc Rosenbaum ◽  
Marlena Duchniewicz ◽  
Sola Kim ◽  
Shenyuan L. Zhang ◽  
...  
Keyword(s):  
B Cells ◽  
Calcium Homeostasis ◽  
Integrin Activation ◽  
Antibody Secretion
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